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新型不織布親水性改質在抑菌防制之研究 = Studies of nove...

國立高雄大學生物科技研究所

新型不織布親水性改質在抑菌防制之研究 = Studies of novel hydrophilic-modified nonwoven on antimicrobial control

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Studies of novel hydrophilic-modified nonwoven on antimicrobial control
作者:	楊智升,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	2008[民97]
面頁冊數:	89面圖，表 : 30公分;
標題:	不織布
標題:	Mycobacterium tuberculosis
附註:	指導教授：林順富
附註:	參考書目：面74-78
附註:	附錄：1.織布不同編織方式示意圖；2.Schiff base reaction
摘要註:	本實驗室自戶外所得之土壤分離出一株黴菌菌種，編號RP-46可分泌紅色色素於胞體外，此色素以紅色為主。基於此紅色抗生物質僅專一性地針對革蘭氏陽性菌具抑制能力，在高雄榮總所委託試驗，對院內感染嚴重的Staphylococcus aureus (ORSA)、Vancomycin-resistant Enterococcus (VRE)及多重抗藥性(multidrug-resistant簡稱：MDR)之Mycobacterium tuberculosis (結核桿菌) 等，皆能在極低之作用濃度下 (MIC在ppb下) 有良好之抑制效果。RP-46生產紅色抗生物質已被精製出，此結構也已被解析出，是一喹諾 (quinolone) 結構的化合物，並具有雙羰基的官能基。基於此羰基結構，我們已能將此紅色色素抗菌物質共價固定於不織布膜上，其最適操作條件如下：於25%戊二醛 90℃，150rpm處理60分後，經丙酮清洗後，再以20%聚乙烯亞胺(PEI)於40℃，150 rpm進行反應二小時，最後添加紅色抗菌物質以150 rpm 30℃恆溫震盪一天，便可獲得最佳的固定效率。不織布經戊二醛及PEI反應處理後，經過持續6次(每次約浸泡12小時)的測試，仍然保有其抗菌的活性。顯見此戊二醛及PEI共聚法可有效地將此紅色抗生物質固定於不織布薄膜上。抑菌試驗發現，經固定化色素的不織布，於SEM檢測發現受測菌被緊密結合於不織布上，顯示其作用機制可能與細胞壁結合有關。由於固定於不織布上的抗菌物質仍具有一未反應的羰基，尚仍再與酵素產生鍵結，形成一三明治的特殊結構，未來也可應用於生物感測器及人工敷料的開發上。 We have discovered a kind of mold “RP-46” in our laboratory; it could secrete extracellular pigment. The main expression color of this pigment is red. Based on this research that red-antibiotics could suppress Gram-positive bacteria specifically, we commissioned Kaohsiung Veteran General Hospital to apply the experiment. The red-antibiotics could restrain several severe infections (such as ORSA, VRE, MDR and Mycobacterium tuberculosis, etc.) in hospital successfully when it was in extremely low density. We have refined the red-antibiotics of RP-46; This structure has been also analyzed, one of the compounds is quinolone’s structure which owns the functional base of the double carbonyl. It might be a marvelous anti-tuberculosis medicine in incoming future. We had been able to coat the red-antibacterial covalence on the nonwoven, the most suitable manipulative conditions is down below: Processing it for 60 minutes by 25% glutardialdehyde, 90°C and 150 rpm is the first step; and then after washing by acetone, processing it for two hour again by 20% PEI, 40°C and 150 rpm is the second step. The last step of processing schedule to get the best stable performance is adding the red antibacterial substance and shaking it for one day by 150 rpm 30°C constantly. After dealing with glutardialdehyde and PEI, and the continuous processing steps for 6 times (Each time it needs to be soaked for 12 hours approximately), the nonwoven could still keep its antibacterial activeness.It was obvious to see that the method of glutardialdehyde and PEI copolymerization could be able to firm the red-antibiotics on the nonwoven efficiently. We had found that the nonwoven with stationary red-antibiotics and the targeting bacteria stuck together throughout the SEM examination on the bacteriostasis’ test. It showed its function was related with cell wall binding.Because

新型不織布親水性改質在抑菌防制之研究 = Studies of novel hydrophilic-modified nonwoven on antimicrobial control
楊, 智升

新型不織布親水性改質在抑菌防制之研究 = Studies of novel hydrophilic-modified nonwoven on antimicrobial control / 楊智升撰 - [高雄市] : 撰者, 2008[民97]. - 89面 ; 圖，表 ; 30公分.
指導教授：林順富參考書目：面74-78附錄：1.織布不同編織方式示意圖；2.Schiff base reaction.
不織布Mycobacterium tuberculosis

新型不織布親水性改質在抑菌防制之研究 = Studies of novel hydrophilic-modified nonwoven on antimicrobial control
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