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轉殖阿拉伯芥中過量表現AtFd3可藉由過氧化物生成促進根部發育 = Ov...

國立高雄大學生物科技研究所

轉殖阿拉伯芥中過量表現AtFd3可藉由過氧化物生成促進根部發育 = Overexpression AtFd3 in transgenic Arabidopsis promotes root development through ROS generation

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Overexpression AtFd3 in transgenic Arabidopsis promotes root development through ROS generation
作者:	詹喬瑋,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	2008[民97]
面頁冊數:	48面圖，表 : 30公分;
標題:	根部發育
標題:	Ferredoxin
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/88075855698567922313
附註:	指導教授：葛孟杰
附註:	參考書目：面37-43
摘要註:	硫鐵蛋白廣泛存於植物體內，並且是參與各不同部位光合作用系統或非光合作用系統的電子攜帶者。阿拉伯芥光合組織相關的硫鐵蛋白可分為AtFd1 及AtFd2 兩種(亦稱為葉型態硫鐵蛋白)。葉型態的硫鐵蛋白可以將光合作用中光系統I 的電子傳遞給線性電子流(linear electron flow, LEF)的NADPH 或thioredoxin，亦可以將電子傳遞給循環電子流(cyclic electron flow, CEF)的Cytochrome b6f。而非光合作用相關硫鐵蛋白則分成AtFd3 及AtFd4(亦稱為根型態硫鐵蛋白)，通常存在於儲存組織，如種子、果實及根部的澱粉體(amyloplast)中。在先前的研究中，我們發現轉殖甜椒的Fd1 可以促進阿拉伯芥根部累積過氧化物並進而促進根毛的發育。因此，為了研究根型態硫鐵蛋白是否也參與根部發育，本論文利用花椰菜鑲嵌病毒的35S 啟動子持續表現AtFd3，觀察轉殖阿拉伯芥其根部發育以及相關的基因表現。結果顯示兩種轉植株的根毛平均長度皆比野生型根毛的長度長，數量也比野生型的根毛多。轉殖株根部中的過氧化物含量皆高於野生型將近兩倍的含量。而透過反轉錄聚合酶連鎖反應的結果得知，在兩種轉殖株的根部中的根毛發育相關基因與氧化還原調節基因都因為促使過氧化物的累積而被誘導並進一步的促進了根毛的發育。根據以上的結果，我們認為植物的根在發育時期需要藉由AtFd3 產生的過氧化物作為訊息傳遞的分子而促使其根毛及根的發育。 Ferredoxin generally exists in whole plant tissue known as an electron carrier in the photosynthetic system and non-photosynthetic system in various organisms. The photosynthesis-associated Fds in Arabidopsis consist of AtFd1 and AtFd2, known as Leaf-type Fd (LT-Fd), that exist in all green tissues. LT-Fd catalyzes electron transfer from photosystem I (PSI) to NADH or thioredoxin for linear electron flow (LEF), and Cytochrome b6f for cyclic electron flow (CEF) in chloroplasts. The non-photosynthesis associated Fds consist AtFd3 and AtFd4 that usually exist in the amyloplast of storage tissue, such as seed, fruit and root, known as Root-type Fd (RT-Fd). In previous studies, the plant ferredoxin like protein (pflp) gene belonged to the LT-Fd of sweet pepper was introduced into the Arabidopsis and then promoted root hair growth through the enhanced production of reactive oxygen species (ROS). In this thesis, CaMV 35S promoter was used to drive the RT-Fd gene of Arabidopsis thaliana, AtFd3, in the Arabidopsis, and studied the effect of AtFd3 in the root development. The root hair length and the content of ROS in the root of two transgenic lines were both apparently increased. In the roots of two transgenic lines, the root hair development-related and redox-regulated gene transcriptional activities were mediated by the higher levels of the ROS to influence root hair growth. In this study, we present a possible role of AtFd3 in the plant root development.

轉殖阿拉伯芥中過量表現AtFd3可藉由過氧化物生成促進根部發育 = Overexpression AtFd3 in transgenic Arabidopsis promotes root development through ROS generation
詹, 喬瑋

轉殖阿拉伯芥中過量表現AtFd3可藉由過氧化物生成促進根部發育 = Overexpression AtFd3 in transgenic Arabidopsis promotes root development through ROS generation / 詹喬瑋撰 - [高雄市] : 撰者, 2008[民97]. - 48面 ; 圖，表 ; 30公分.
指導教授：葛孟杰參考書目：面37-43.
根部發育Ferredoxin

轉殖阿拉伯芥中過量表現AtFd3可藉由過氧化物生成促進根部發育 = Overexpression AtFd3 in transgenic Arabidopsis promotes root development through ROS generation
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