製備具標靶葉酸受體診斷及治療的多功能pluronic修飾四氧化三鐵 = ...
國立高雄大學生物科技研究所

 

  • 製備具標靶葉酸受體診斷及治療的多功能pluronic修飾四氧化三鐵 = Folate-Mediated Pluronic Modified Fe3O4 for Combined Diagnosis and Therapy
  • 紀錄類型: 書目-語言資料,印刷品 : 單行本
    並列題名: Folate-Mediated Pluronic Modified Fe3O4 for Combined Diagnosis and Therapy
    作者: 林嘉鈞,
    其他團體作者: 國立高雄大學
    出版地: [高雄市]
    出版者: 撰者;
    出版年: 2009[民98]
    面頁冊數: [8], 67面圖,表 : 30公分;
    標題: Pluronic F127
    標題: Diagnosis
    電子資源: http://handle.ncl.edu.tw/11296/ndltd/66533734059769910024
    附註: 指導教授:陳振興
    附註: 參考書目:面66-67
    摘要註: 核磁共振造影最大的貢獻在於能在不同的組織和不同的環境下,呈現出有差異的顯影效果,因此被用來診斷組織的病變與否。本研究中,利用Pluronic®高分子F127(PF127) 的PPO疏水端攜帶疏水性藥物,與水溶性的四氧化三鐵(PAAIO)反應,並在PF127親水端接枝上葉酸(folic acid, FA)做為標靶癌細胞的配體分子,形成一個具備診斷、治療、標靶的多功能磁性微胞(FA-PF127-PAAIO)。並以無葉酸標的的磁性微胞(PF127-PAAIO)作為對照組。使用紅外線光譜儀觀測磁性微胞酯鍵的吸收。紫外線吸收光譜儀觀察到葉酸的特殊吸收訊號。修飾後的磁性微胞從TEM、DLS觀察粒徑大小,在介面電位也有明顯的變化。AAS組成比例分析磁性微胞的含鐵量。超導量子干涉儀分析上,由磁滯曲線測得超順磁性以及飽和磁化率。細胞實驗裡,我們利用流式細胞儀與體外磁振造影實驗分析含葉酸的FA-PF127-PAAIO標的癌細胞的表現,初步的結果說明FA-PF127-PAAIO有標靶葉酸受體的能力。 Magnetic resonance (MR) tracking of superparamagnetic iron oxide labeled cells is a relatively new technique to non-invasively determine the cancer cells.In this study, Pluronic® F127 (PF127), which is capable of encapsulating hydrophobic drug due to its PPO hydrophobic segments, was proceeded a condensation reaction with water-soluble polyacrylic acid bound iron oxide (PAAIO). A tumor-specific targeting ligand, folic acid (FA), was also grafted onto PF127-PAAIO to give FA-PF127-PAAIO magnetic micelles. A multifunctional FA-PF127-PAAIO can be used as combined diagnosis and therapy purposes specifically targeting to cancer cells. PF127-PAAIO magnetic micelles without folic acid were used as a control group. According to IR and UV absorption spectra, we ensured the successful preparation of the magnetic micelles with polymeric compartments for carrying a drug. The hysteresis curves of SQUID magnetometer analysis also showed that these magnetic micelles were superparamagnetic and a saturation magnetization was obtained in FA-PF127-PAAIO and PF127-PAAIO.Internalization of FA-PF127-PAAIO in targeted cells (KB, A549 cells) was observed by flow-cytometric analysis and in vitro MR imaging. These preliminary results demonstrated that FA-PF127-PAAIO have the ability to target folate receptor.
館藏
  • 2 筆 • 頁數 1 •
 
310001797656 博碩士論文區(二樓) 不外借資料 學位論文 TH 008M/0019 420228 4448 2009 一般使用(Normal) 在架 0
310001797649 博碩士論文區(二樓) 不外借資料 學位論文 TH 008M/0019 420228 4448 2009 c.2 一般使用(Normal) 在架 0
  • 2 筆 • 頁數 1 •
評論
Export
取書館別
 
 
變更密碼
登入