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Multidimensional approaches to chemical genetics.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Multidimensional approaches to chemical genetics.
作者:	Wagner, Bridget Kelly.
面頁冊數:	167 p.
附註:	Adviser: Stuart L. Schreiber.
附註:	Source: Dissertation Abstracts International, Volume: 64-05, Section: B, page: 2181.
Contained By:	Dissertation Abstracts International64-05B.
標題:	Chemistry, Biochemistry.
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3091715
ISBN:	049639424X

Multidimensional approaches to chemical genetics.
Wagner, Bridget Kelly.

Multidimensional approaches to chemical genetics. [electronic resource] - 167 p.

Adviser: Stuart L. Schreiber.

Thesis (Ph.D.)--Harvard University, 2003.

Chemical genetics makes use of small molecules to perturb protein function. By direct analogy to classical genetics and its use of genetic mutation, the result of chemical-genetic identification of small-molecule perturbations is a richer understanding of gene products and their function and interplay within the cell. Previous efforts toward chemical-genetic discovery have involved the design of an assay, the readout of which involves the activity of one's "favorite" protein. In this case, however, the use of chemical genetics virtually ends at the identification of small-molecule inhibitors or activators of this particular protein function. We have endeavored to broaden the scope of chemical genetics to the study of large collections of small molecules across multiple phenotypic assays.

ISBN: 049639424XSubjects--Topical Terms:

226900
Chemistry, Biochemistry.

Multidimensional approaches to chemical genetics.
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520 # $a Chemical genetics makes use of small molecules to perturb protein function. By direct analogy to classical genetics and its use of genetic mutation, the result of chemical-genetic identification of small-molecule perturbations is a richer understanding of gene products and their function and interplay within the cell. Previous efforts toward chemical-genetic discovery have involved the design of an assay, the readout of which involves the activity of one's "favorite" protein. In this case, however, the use of chemical genetics virtually ends at the identification of small-molecule inhibitors or activators of this particular protein function. We have endeavored to broaden the scope of chemical genetics to the study of large collections of small molecules across multiple phenotypic assays.
520 # $a We describe the development of a chemical-genetic technology platform that enables current and future screening efforts in high throughput. This newly acquired capability has facilitated the rapid and efficient performance of multiple chemical-genetic modifier screens, based on the detection of 5-bromodeoxyuridine (BrdU) incorporation. We not only have identified several small molecules with intriguing suppressive activity toward protein tyrosine kinase inhibitors, but also have investigated the relationships between these multiple screens. We have developed a data analysis platform based on the clustering of screening data, resulting in the generation of small-molecule fingerprints for each compound. This multidimensional approach shows great promise in the further characterization of cellular states and their responses to small-molecule perturbation.
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