國立高雄大學圖資館 |

登入

回首頁

Structural mechanisms of DNA replication fidelity.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Structural mechanisms of DNA replication fidelity.
作者:	Johnson, Sean James.
面頁冊數:	152 p.
附註:	Source: Dissertation Abstracts International, Volume: 64-10, Section: B, page: 4918.
附註:	Supervisor: Lorena S. Beese.
Contained By:	Dissertation Abstracts International64-10B.
標題:	Chemistry, Biochemistry.
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3109010
ISBN:	0496565559

Structural mechanisms of DNA replication fidelity.
Johnson, Sean James.

Structural mechanisms of DNA replication fidelity. [electronic resource] - 152 p.

Source: Dissertation Abstracts International, Volume: 64-10, Section: B, page: 4918.

Thesis (Ph.D.)--Duke University, 2003.

Mechanisms of polymerase fidelity have been further explored by studying the effect of a replication error on the BF•DNA complex. Each of the twelve possible DNA mismatches has been examined at the polymerase active site, several by incorporation into the crystal under mutagenic conditions. The various observed mismatch conformations include several novel structures. DNA mismatches are shown to produce disrupted, disordered, or frayed complexes when bound at the polymerase active site. The conformational response of the polymerase to misincorporated nucleotides highlights various mechanisms of error recognition that cause stalling of DNA synthesis, facilitating excision of the errant base by an associated exonuclease activity or termination of synthesis by dissociation of the DNA from the polymerase. The relative significance of each of the observed conformational changes on continued DNA synthesis has been tested in the polymerase crystals. Several mismatches were successfully extended along the polymerase surface. The resulting product complexes demonstrate how the polymerase can sense replication errors for several rounds of synthesis following misinsertion.

ISBN: 0496565559Subjects--Topical Terms:

226900
Chemistry, Biochemistry.

Structural mechanisms of DNA replication fidelity.
LDR:03450nmm _2200277 _450 001 162259
005 20051017073427.5
008 230606s2003 eng d
020 $a 0496565559
035 $a 00148760
035 $a 162259
040 $a UnM $c UnM
100 0 $a Johnson, Sean James. $3 227385
245 1 0 $a Structural mechanisms of DNA replication fidelity. $h [electronic resource]
300 $a 152 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-10, Section: B, page: 4918.
500 $a Supervisor: Lorena S. Beese.
502 $a Thesis (Ph.D.)--Duke University, 2003.
520 # $a Mechanisms of polymerase fidelity have been further explored by studying the effect of a replication error on the BF•DNA complex. Each of the twelve possible DNA mismatches has been examined at the polymerase active site, several by incorporation into the crystal under mutagenic conditions. The various observed mismatch conformations include several novel structures. DNA mismatches are shown to produce disrupted, disordered, or frayed complexes when bound at the polymerase active site. The conformational response of the polymerase to misincorporated nucleotides highlights various mechanisms of error recognition that cause stalling of DNA synthesis, facilitating excision of the errant base by an associated exonuclease activity or termination of synthesis by dissociation of the DNA from the polymerase. The relative significance of each of the observed conformational changes on continued DNA synthesis has been tested in the polymerase crystals. Several mismatches were successfully extended along the polymerase surface. The resulting product complexes demonstrate how the polymerase can sense replication errors for several rounds of synthesis following misinsertion.
520 # $a The accurate transfer of genetic material from one generation to the next is critically dependent on high-fidelity replication by DNA polymerases. Errors in DNA synthesis, if uncorrected, result in mutations that can have deleterious consequences for the cell. DNA polymerases achieve remarkable accuracy during replication of the DNA template strand. For every one million nucleotides synthesized onto the complementary DNA primer strand, the polymerase may only incorporate a single replication error.
520 # $a The structural basis for this fidelity has been examined using a Family A (Pol I) DNA polymerase from a thermostable strain of Bacillus stearothermophilus (Bacillus fragment, BF). BF is able to synthesize DNA while in the crystalline state, undergoing large motions without disrupting the crystal lattice. This activity, which is shown to be both accurate and processive in the crystal, has allowed for the structural determination of product complexes representing multiple rounds of accurate DNA synthesis by x-ray crystallography. Several distinct conformational states are observed in the BF•DNA crystals that represent various steps along the reaction pathway, which have clarified and more fully delineated molecular details of the dynamic process employed to achieve accurate replication.
590 $a School code: 0066.
650 # 0 $a Chemistry, Biochemistry. $3 226900
650 # 0 $a Biology, Molecular. $3 226919
710 0 # $a Duke University. $3 226880
773 0 # $g 64-10B. $t Dissertation Abstracts International
790 $a 0066
790 1 0 $a Beese, Lorena S., $e advisor
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://libsw.nuk.edu.tw/login?url=http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3109010 $z http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3109010