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Elasticity of F-actin networks

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Elasticity of F-actin networks
作者:	Gardel, Margaret Lise.
面頁冊數:	169 p.
附註:	Adviser: David A. Weitz.
附註:	Source: Dissertation Abstracts International, Volume: 65-05, Section: B, page: 2457.
Contained By:	Dissertation Abstracts International65-05B.
標題:	Physics, Condensed Matter.
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3131843
ISBN:	0496790838

Elasticity of F-actin networks
Gardel, Margaret Lise.

Elasticity of F-actin networks [electronic resource] - 169 p.

Adviser: David A. Weitz.

Thesis (Ph.D.)--Harvard University, 2004.

In Chapter 4, we study solutions of entangled F-actin. We elucidate the microscopic origins of bulk elasticity using microrheology techniques. We also show that multiple particle tracking can also probe the dynamics of the F-actin solution microstructure.

ISBN: 0496790838Subjects--Topical Terms:

226939
Physics, Condensed Matter.

Elasticity of F-actin networks
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520 # $a In Chapter 4, we study solutions of entangled F-actin. We elucidate the microscopic origins of bulk elasticity using microrheology techniques. We also show that multiple particle tracking can also probe the dynamics of the F-actin solution microstructure.
520 # $a In Chapter 6, we study the elastic properties of F-actin networks assembled with a compliant cross-linker, filamin. We study the mechanical properties of these networks at physiological conditions and find that these are in qualitative agreement with mechanical measurements of living cells. Finally, we find that deletion of a flexible, unstructured 'hinge' region of the filamin protein dramatically affects the mechanical properties of F-actin networks cross-linked with filamin.
520 # $a This thesis presents a study of the elasticity and microstructure of three filamentous actin (F-actin) based materials. Using bulk rheology, microrheology, multiple particle tracking and imaging techniques, we study the microscopic origins of the mechanical properties of F-actin networks.
520 # $a We briefly introduce aspects of F-actin and rheology essential to provide a background for and motivate this thesis in Chapter 1. In Chapter 2, we describe the materials and methods used. An introduction to microrheology is given in Chapter 3.
520 # $a We explore the effect of rigid, incompliant chemical cross-links between actin filaments in Chapter 5. We explore changes in the network microstructure as the concentration of cross-links is varied. We find that the elastic stiffness of these networks is extremely sensitive to small changes in cross-link density. Despite this large variation, the linear viscoelasticity of all networks can be scaled onto a universal master curve; this scaling reveals that the mechanical dissipation of the networks is due to thermal fluctuations of F-actin. At large stresses, the mechanical stiffness of these networks diverges. The form of this stress stiffening response is consistent with the non-linear force extension of a single semi-flexible polymer. Thus, over a large range of conditions, the linear and nonlinear mechanical response of rigidly cross-linked networks is entropic in origin. Finally, at very low cross-link and filament densities, we observe a transition to a qualitatively different type of elasticity; this is consistent with a transition to an enthalpic network elasticity dominated by bending of F-actin.
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