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The hydrodynamics of DNA electrokine...

Ferree, Sean Matthew.

The hydrodynamics of DNA electrokinetic stretch and relaxation.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	The hydrodynamics of DNA electrokinetic stretch and relaxation.
作者:	Ferree, Sean Matthew.
面頁冊數:	220 p.
附註:	Chair: Harvey W. Blanch.
附註:	Source: Dissertation Abstracts International, Volume: 65-09, Section: B, page: 4705.
Contained By:	Dissertation Abstracts International65-09B.
標題:	Engineering, Chemical.
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3146845
ISBN:	0496057693

The hydrodynamics of DNA electrokinetic stretch and relaxation.
Ferree, Sean Matthew.

The hydrodynamics of DNA electrokinetic stretch and relaxation. - 220 p.

Chair: Harvey W. Blanch.

Thesis (Ph.D.)--University of California, Berkeley, 2004.

Our measurements show that DNA experiences a larger stretching force in a semi-dilute polymer solution when hydrodynamic interactions are screened, compared to free solution and approaches free-draining behavior. The relaxation o f many molecules of different length in this polymer solution show that the slowest relaxation mode scales with DNA length as tauR &sim; L2.17+/-0.17, consistent with the Rouse model in a good solvent. Finally, both the steady stretching and dynamic relaxation of single DNA molecules are predicted well by the Stigter theory using a hydrodynamic screening length found from effective medium theory equal to 21nm.

ISBN: 0496057693Subjects--Topical Terms:

226989
Engineering, Chemical.

The hydrodynamics of DNA electrokinetic stretch and relaxation.
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245 1 4 $a The hydrodynamics of DNA electrokinetic stretch and relaxation.
300 $a 220 p.
500 $a Chair: Harvey W. Blanch.
500 $a Source: Dissertation Abstracts International, Volume: 65-09, Section: B, page: 4705.
502 $a Thesis (Ph.D.)--University of California, Berkeley, 2004.
520 # $a Our measurements show that DNA experiences a larger stretching force in a semi-dilute polymer solution when hydrodynamic interactions are screened, compared to free solution and approaches free-draining behavior. The relaxation o f many molecules of different length in this polymer solution show that the slowest relaxation mode scales with DNA length as tauR &sim; L2.17+/-0.17, consistent with the Rouse model in a good solvent. Finally, both the steady stretching and dynamic relaxation of single DNA molecules are predicted well by the Stigter theory using a hydrodynamic screening length found from effective medium theory equal to 21nm.
520 # $a Our results on the steady electrophoretic stretching of tethered DNA in free solution are the first conclusive experimental demonstration that DNA is not free-draining in the presence of both electric and non-electric forces. DNA stretches the same in an electric field E, as it does in a uniform flow field with linear velocity equal to muE, where mu is the electrophoretic mobility, corroborating the theory of hydrodynamic equivalence. In addition, our data are described well without adjustable parameters by the detailed theory of electrophoretic stretch developed by Stigter and Bustamante.
520 # $a This dissertation describes experimental and theoretical investigations of the effect of long-ranged hydrodynamic interactions on the electrophoretic stretch and relaxation of single molecules of DNA. Using semi-conductor manufacturing techniques we have fabricated a microfluidic device consisting of an array of micron-sized posts topped with a thin layer of gold. We tether single linear DNA molecules by one end to these gold posts and measure the extent of stretching in an electric field using epi-fluorescent video microscopy. We have studied the steady stretching of DNA in free solution, as well as the steady stretching and dynamic relaxation in a semi-dilute solution of the hydrophyllic polymer hydroxyethyl cellulose.
520 # $a Using finite element modeling, and Monte-Carlo simulations we showed that the field perturbation by the microfabricated posts does not significantly effect the experimental results on DNA stretching. Particle image velocimetry demonstrated that electroosmotic flow is generated by the silicon surfaces in our devices, but that this flow can be eliminated by coating all oxide surfaces with a brush of linear polyacrylamide.
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790 1 0 $a Blanch, Harvey W., $e advisor
791 $a Ph.D.
792 $a 2004
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