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點帶石斑魚腦細胞株(GBC4)分子表現分析 = Molecular Ex...

國立高雄大學生物科技研究所

點帶石斑魚腦細胞株(GBC4)分子表現分析 = Molecular Expression of a Clonal Cell Line (GBC4) Derived from Brain of Orange-Spotted Grouper, Epinephelus coioides

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Molecular Expression of a Clonal Cell Line (GBC4) Derived from Brain of Orange-Spotted Grouper, Epinephelus coioides
作者:	黃靖貽,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	2008[民97]
面頁冊數:	52面圖、表 : 30公分;
標題:	分子標記
標題:	cell line
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/36473065424058858120
附註:	指導教授：溫秋明
附註:	參考書目：面
摘要註:	魚類的中樞神經系統終生都在發育，因此很適合做為中樞神經發育的研究材料，而本實驗室先前已建立了多株魚類的腦細胞株。為了瞭解這些細胞株是具有神經幹細胞的特性以及其發育的潛力，鑑定這些細胞株的分子表現是必要的。利用RT-PCR以及西方點墨雜交等技術，本論文驗證由點帶石斑魚腦所得細胞株GBC4具有astroglial細胞的分子表現，包括glial fibrillary acidic protein (GFAP)和vimentin。此外，GBC4細胞株同時也表現寡突觸前驅細胞特有分子platelet-derived growth factor receptor-α (PDGFR-α) 和proteolipid protein (PLP)；神經細胞分子tyrosine hydroxylase (TH) 和 gap junction connexin 35。先前研究顯示大量tanycytes廣泛存在成熟硬骨魚腦室中，tanycytes可以表現GFAP以及vimentin，因此為了確認GBC4是否可能為tanycyte，同時檢測aromatase B、 brain lipid binding protein、 connexin43 protein、glutamine synthetase、 S100 protein以及Sox2分子表現。結果顯示點帶石斑魚腦所得細胞株GBC4可能為多能性神經前驅細胞。 The central nervous system (CNS) of teleost fish as it develops throughout the lifespan is benetif to the studies for neual development. Previously, several cell lines have developed from fish brain in our lab. For neural development study, we are interesting to know if the cell lines have the characteristics of neural stem cell. Thus, we examined molecular expression of the cells.In this study, a clonal cell line from range-spotted grouper (Epinephelus coioides) was revealed that has astroglial markers, glial fibrillary acidic protein (GFAP), vimentin oligodendrocyte markers, platelet-derived growth factor receptor-α (PDGFR-α) and proteolipid protein (PLP), and neuron markers, tyrosine hydroxylase (TH) and gap junction connexin 35 by RT-PCR and western blot. In addition, markers for radial glial cells as well as tanycytes including aromatase B, brain lipid binding protein, connexin43 protein, glutamine synthetase, S100 protein and Sox2 were demonstrated exhibiting in GBC4 cells. According to the molecular expression, GBC4 cells were recognized astanycytes. Tanycytes in fish CNS are stem cells suggesting that GBC4 cells neural stem cells.

點帶石斑魚腦細胞株(GBC4)分子表現分析 = Molecular Expression of a Clonal Cell Line (GBC4) Derived from Brain of Orange-Spotted Grouper, Epinephelus coioides
黃, 靖貽

點帶石斑魚腦細胞株(GBC4)分子表現分析 = Molecular Expression of a Clonal Cell Line (GBC4) Derived from Brain of Orange-Spotted Grouper, Epinephelus coioides / 黃靖貽撰 - [高雄市] : 撰者, 2008[民97]. - 52面 ; 圖、表 ; 30公分.
指導教授：溫秋明參考書目：面.
分子標記cell line

點帶石斑魚腦細胞株(GBC4)分子表現分析 = Molecular Expression of a Clonal Cell Line (GBC4) Derived from Brain of Orange-Spotted Grouper, Epinephelus coioides
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