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磁性誘導凝膠之製備及在許旺細胞培養之應用 = Preparation o...

國立高雄大學生物科技研究所

磁性誘導凝膠之製備及在許旺細胞培養之應用 = Preparation of magnet-induced gels and their applicationsfor growth of Schwann Cells

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Preparation of magnet-induced gels and their applicationsfor growth of Schwann Cells
作者:	王文萱,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	2009[民98]
面頁冊數:	109面圖、表 : 30公分;
標題:	天然生物活性高分子
標題:	Schwann cells
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/87587805119663198945
附註:	指導教授：鍾宜璋
附註:	參考書目：面
摘要註:	本研究乃結合磁性奈米分子與生物高分子發展出一種磁場誘導成膠的系統。磁性凝膠的形成是以物理交聯和沉澱的方式，加上外部磁場的引導，可以非常容易地控制及誘導分粒子到理想的地方形成凝膠，以作為細胞支架和組織再生修復之用。在材料設計中，氧化鐵奈米粒子的磁性核扮演一個重要的角色，它擁有超順磁的特性與生物相容性；此外，利用天然的聚電解質當做外部塗層，可以加強細胞的附著和生長。實驗作法上利用化學共沉澱法，製備出水相分散的磁性奈米粒子(Fe3O4)，並使用界面活性劑保護，再利用逐層組裝包覆相異電荷的生物可分解性高分子，如幾丁聚醣(Chitosan)和褐藻酸(Alginate)，以形成穩定的生物可分解性分子包裹的表面，稱之為磁性凝膠。之後將陽離子和陰離子奈米粒子膠體依不同比例混合，控制其凝膠形態，最後外加磁場誘導其沉積於基材上，並於基板上置入許旺氏神經細胞，利用顯微鏡觀察細胞生長和貼附情形。磁性凝膠藉由X 光繞射分析（XRD）、穿透式電子顯微鏡（TEM）、界面電位分析（Zeta potential）、X 光光電子能譜儀（XPS）等鑑定其性質。由XRD 結果比對文獻後證明本實驗合成的磁性奈米粒子為Fe3O4 的型態；而TEM 觀察結果顯示，磁性奈米粒子大小約為10-20nm，包覆天然高分子後其粒徑變大；測其表面電位得到CS 包覆粒子為+31.7 mV，而AA 包覆粒子為-40.3 mV，顯示其在水溶液中可均勻分散且容易造成異電荷相吸；還有XPS 的能譜變化分析，可證明天然高分子確實包覆於磁性奈米粒子的表面。將兩種相異電荷的磁性凝膠依等比例混合時，在外加磁場作用下可形成一均勻薄膜，甚至可以利用磁場誘導其沿磁力線沉積成纖維狀結構，初步在其結構上培養許旺氏神經細胞，發現細胞會吸附在此薄膜上生長。此外，各種不同比例沉積的薄膜，對於神經細胞的貼附程度及生長方向也不盡相同，這都表示未來利用磁性粒子與生物活性分子的結合，可以做為神經導管應用於周邊神經組織之修復。 The study focused on a combination of magnetic nanoparticles and bioactive polymers to develop a magnet-induced gel-forming system. The magnetic gel was able to form a gel by physical crosslink and precipitation, as well as by external magnetic field induction. Thus, we can accessibly transfer particles to desired spots under well control and induction in order to fabricate a scaffold for cell growth and tissue reparation. In the design of materials, iron oxide nanoparticles played an important role because of their superparamagneticity and biocompatibility. On the other hand, the cell growth and attachment was able to be enhanced using natural polyelectrolytes as the outer coating. Experimentally, the well-dispersed magnetic Fe3O4 nanoparticals were prepared by a chemical coprecipitation method. The particle surfaces were passivated and protected using polyelectrolyte surfactants, and then were coated by the opposite-charged biodegradable polyelectrolyte (i.e., chitosan, alginate). As mixing different ratios of chitosan-coated to alginate-coated particles, we were able to control the morphology of gel forming on a substrate with a suitable interaction with the external magnet. The substrates were then contacted with Schwann cells in a certain time to observe the cell attachment and growth. The magnetic nanoparticles were analyzed and characterized by XRD, TEM, zeta potential meter, XPS, and so on. Fe3O4 structure and crystalline was proved by the XRD analysis as literatures reported. TEM photographs illustrated the nanoparticles sizing as 10-20 nm in diameter. And also the size was increased as being encapsulated by natural polymers. Zeta potential analysis showed the CS-coated particles displayed a highly positive potential as +31.7 mV, while the AA-coated particles as -40.3 mV, demonstrating their well-dispersion and highly attractive interaction between each other. XPS analysis on the composition changes for the particle surfaces also provided an evidence of successful polymer coating. As injecting and mixing the equivalent ratio between the two opposite-charged nanoparticle suspensions, we were able to find the nanoparticles gel-forming to a homogeneous thin film on a substrate under control by an external magnetic field. As changing the magnetic direction and quantity, a fiber-like microstructure was specified. Preliminary cell attachment tests on the fiber-like films, Schwann cells tended to attach and grow along the fiber orientation. All the results demonstrated the gel to be a potential template for repairing peripheral nerve system.

磁性誘導凝膠之製備及在許旺細胞培養之應用 = Preparation of magnet-induced gels and their applicationsfor growth of Schwann Cells
王, 文萱

磁性誘導凝膠之製備及在許旺細胞培養之應用 = Preparation of magnet-induced gels and their applicationsfor growth of Schwann Cells / 王文萱撰 - [高雄市] : 撰者, 2009[民98]. - 109面 ; 圖、表 ; 30公分.
指導教授：鍾宜璋參考書目：面.
天然生物活性高分子Schwann cells

磁性誘導凝膠之製備及在許旺細胞培養之應用 = Preparation of magnet-induced gels and their applicationsfor growth of Schwann Cells
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