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SiO2@PAA粒子的合成與其吸附脫附蛋白質能力的探討 = Synthe...

國立高雄大學應用化學系碩士班

SiO2@PAA粒子的合成與其吸附脫附蛋白質能力的探討 = Syntheses of SiO2@PAA Particles and Their Capability on Adsorption-Desorption of Protein

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Syntheses of SiO2@PAA Particles and Their Capability on Adsorption-Desorption of Protein
作者:	莊博今,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	民99[2010]
面頁冊數:	114面圖，表 : 30公分;
標題:	微波合成
標題:	solid-phase extraction
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/53870399147698291478
摘要註:	本研究的目的在於發展一套可銜接於毛細管電泳管柱前端的線上萃取裝置，以提升現有毛細管電泳法分析蛋白質的偵測極限，並縮短操作時間。所採用的固相萃取裝置為內壁以polyacrylic acid(PAA)修飾的SiO2 毛細管柱，藉由PAA的多重羧基與鹼性蛋白質(如lysozyme)形成穩定的靜電作用力，將樣品中的蛋白質萃取，再以調控pH 或離子強度的方式將蛋白質由PAA 脫附濃縮。我們針對SiO2 顆粒表面在不同條件下與PAA 反應(SiO2@PAA)的效果，比較傳統加熱和微波加熱，以及不同PAA 鏈長和SiO2 粒徑對PAA 修飾量的影響。應用SiO2@PAA 顆粒研究所得到的最佳實驗條件，可將PAA 修飾於毛細管柱內壁上進行lysozyme 的萃取濃縮。研究結果顯示，SiO2@PAA顆粒對lysozyme的萃取量較未修飾的SiO2顆提升了7倍，而針對100 ppm的lysozyme，濃縮效果可達15倍，但所吸附的lysozyme只有60%左右脫附。至於經PAA修飾後的毛細管柱，其對lysozyme的萃取量則提升了近一倍。研究結果顯示，未來進一步改進濃縮效果後，以PAA修飾的毛細管柱可做為毛細管電泳分析蛋白質時的線上濃縮工具。 The purpose of this study was to develop an online solid-phase extraction method that can increase the detection limit of capillary electrophoresis for analyzing protein. The method involved a SiO2 capillary column that inner surface fabricated with polyacrylic acid(PAA). Lysozyme in the sample was absorbed on the fabricated surface by ionic interactions between PAA and lysozyme. Adsorption and desorption of lysozyme was achieved by manipulating pH and ionic strength of sample solution. Several experimental conditions, conventional and microwave heating methods,size of PAA and SiO2 particle, were studied to maximize concentrated efficiency of lysozyme. SiO2 particle was used to derive the optimized conditions for fabrication. The optimized parameter was further applied on fabrication of capillary column.The amount of lysozyme extracted by PAA fabricated SiO2 particle (SiO2@PAA) was 7 times higher than that of corresponding bare SiO2 particle. Solution contained 100 ppm lysozyme was concentrated 15 times by SiO2@PAA. Nevertheless, only around 60% of adsorbed lysozyme was desorbed from SiO2@PAA. The amount of lysozyme that condensed by capillary column was double after the column fabricated with PAA. The result indicted that, after further improvement on concentrated efficiency, capillary column fabricated with PAA can be potential online solid-phase extraction tool for capillary electrophoresis.

SiO2@PAA粒子的合成與其吸附脫附蛋白質能力的探討 = Syntheses of SiO2@PAA Particles and Their Capability on Adsorption-Desorption of Protein
莊, 博今

SiO2@PAA粒子的合成與其吸附脫附蛋白質能力的探討 = Syntheses of SiO2@PAA Particles and Their Capability on Adsorption-Desorption of Protein / 莊博今撰 - [高雄市] : 撰者, 民99[2010]. - 114面 ; 圖，表 ; 30公分.
參考書目：面.
微波合成solid-phase extraction

SiO2@PAA粒子的合成與其吸附脫附蛋白質能力的探討 = Syntheses of SiO2@PAA Particles and Their Capability on Adsorption-Desorption of Protein
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328 $a 碩士論文--國立高雄大學應用化學系碩士班
330 $a 本研究的目的在於發展一套可銜接於毛細管電泳管柱前端的線上萃取裝置，以提升現有毛細管電泳法分析蛋白質的偵測極限，並縮短操作時間。所採用的固相萃取裝置為內壁以polyacrylic acid(PAA)修飾的SiO2 毛細管柱，藉由PAA的多重羧基與鹼性蛋白質(如lysozyme)形成穩定的靜電作用力，將樣品中的蛋白質萃取，再以調控pH 或離子強度的方式將蛋白質由PAA 脫附濃縮。我們針對SiO2 顆粒表面在不同條件下與PAA 反應(SiO2@PAA)的效果，比較傳統加熱和微波加熱，以及不同PAA 鏈長和SiO2 粒徑對PAA 修飾量的影響。應用SiO2@PAA 顆粒研究所得到的最佳實驗條件，可將PAA 修飾於毛細管柱內壁上進行lysozyme 的萃取濃縮。研究結果顯示，SiO2@PAA顆粒對lysozyme的萃取量較未修飾的SiO2顆提升了7倍，而針對100 ppm的lysozyme，濃縮效果可達15倍，但所吸附的lysozyme只有60%左右脫附。至於經PAA修飾後的毛細管柱，其對lysozyme的萃取量則提升了近一倍。研究結果顯示，未來進一步改進濃縮效果後，以PAA修飾的毛細管柱可做為毛細管電泳分析蛋白質時的線上濃縮工具。 The purpose of this study was to develop an online solid-phase extraction method that can increase the detection limit of capillary electrophoresis for analyzing protein. The method involved a SiO2 capillary column that inner surface fabricated with polyacrylic acid(PAA). Lysozyme in the sample was absorbed on the fabricated surface by ionic interactions between PAA and lysozyme. Adsorption and desorption of lysozyme was achieved by manipulating pH and ionic strength of sample solution. Several experimental conditions, conventional and microwave heating methods,size of PAA and SiO2 particle, were studied to maximize concentrated efficiency of lysozyme. SiO2 particle was used to derive the optimized conditions for fabrication. The optimized parameter was further applied on fabrication of capillary column.The amount of lysozyme extracted by PAA fabricated SiO2 particle (SiO2@PAA) was 7 times higher than that of corresponding bare SiO2 particle. Solution contained 100 ppm lysozyme was concentrated 15 times by SiO2@PAA. Nevertheless, only around 60% of adsorbed lysozyme was desorbed from SiO2@PAA. The amount of lysozyme that condensed by capillary column was double after the column fabricated with PAA. The result indicted that, after further improvement on concentrated efficiency, capillary column fabricated with PAA can be potential online solid-phase extraction tool for capillary electrophoresis.
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