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霍山石斛試管內形態發生及提前開花之誘導 = Induction of i...

國立高雄大學生物科技研究所

霍山石斛試管內形態發生及提前開花之誘導 = Induction of in vitro morphogenesis and early flowering of Dendrobium huoshanense

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Induction of in vitro morphogenesis and early flowering of Dendrobium huoshanense
作者:	李伯倫,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	民100
面頁冊數:	55葉圖，表 : 30公分;
標題:	癒傷組織
標題:	callus
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/76677003214045831468
附註:	參考書目：葉42-55
摘要註:	本論文之研究分二個部份，第一部分為霍山石斛之試管內形態發生，其材料為試管內之實生苗，切取根尖、莖節及葉等作為培植體，於含有不同比例植物生長調節劑之培養基，進行癒傷組織之誘導，以做為更進一步之試驗之材料。試驗所用之基礎培養基為1/2 MS 添加20 g/l蔗糖、1 g/l蛋白腖及4 g/l水晶洋菜。試驗之設計，乃另外添加不同濃度比例之植物生長素2,4-D及細胞分裂素TDZ，以進行形態發生之誘導。經誘導結果，僅根尖於2,4-D 1 mg/l下，可以成功誘導產生癒傷組織。而所獲得之癒傷組織以同樣之培養基繼代後，發現在無任何TDZ存在下，癒傷組織呈現鮮黃色，並得以繼續生長。而這些癒傷組織經由改放置於含NAA另外添加TDZ或BA培養基後，陸續被誘導而形成類原球體。另外，在霍山石斛發根之實驗中，以0.1 mg/l NAA、1 mg/IBA及5 mg/l IBA等處理組之誘導發根反應最佳，平均分別有4、3.6及3.6的發根數。而這些處理組其分芽生長茂盛，且根部發育良好，顯然NAA及IBA對於霍山石斛之發根具有重要之角色。第二部份為霍山石斛試管內提前開花之研究，其材料以具2-3葉，約1公分大小之幼苗為材料，於含有不同比例濃度之植物生長調節劑下，進行試管內提前開花之誘導。基礎培養基內另外添加不同濃度組合之PGR，以NAA配合TDZ、BA、2iP或kinetin。試驗結果顯示，在未添加任何NAA之試驗組中，以BA及kinetin該兩組較早被誘導花苞，其中0.5 mg/l BA平均花苞形成率為1.67個最高，其次為1 mg/l BA 有0.83個，其花苞形成情形較佳，而TDZ組則無任何誘導情形發生。然而，在添加0.5 mg/l NAA試驗組方面，以TDZ及kinetin較早被誘導花苞形成，BA處理組則先大量誘導小植株產生後，於後期陸續被誘導花苞形成。統計其平均花苞形成率，以5 mg/l TDZ 有1.17個最高，5 mg/l BA及1 mg/l BA為0.67及0.5個次之。分析其上述結果，BA在有無NAA誘導下，皆具有誘導作用。在繼代霍山石斛植株時，可由其生理反應推測，霍山石斛本身內生性細胞分裂素可能較高，故能於無生長調節劑之下，即可偶發性試管內開花。但若另外添加外生性細胞分裂素，其試管內提前開花之情形會相對增加。因此，可推測細胞分裂素扮演著試管內開花重要之因子。 The thesis was separated the two sections. To study in vitro morphogenesis of Dendrobium huoshanense is the first experiment. Basal medium contains 1/2 MS salt with vitamin, 20 g/l sucrose, 1 g/l peptone and 4 g/l Gelrite. The combination of 2,4-D with TDZ were also added for induction of callus. Root tip, leaf and stem node were harvested and placed on the medium. Callus was induced and grew well from root tip in the medium with 1 mg/l 2,4-D. Bright yellow callus was subsequently transferred to the medium containing NAA combination with TDZ or BA for induction of somatic embryogenesis. The PLB were induced and regenerated after 3 months. The rooting experiment was subsequently induced by added NAA or IBA. The results revealed that 0.1 mg/l NAA, 1 mg/l IBA and 5 mg/l IBA, with 4, 3.6 and 3.6 roots per tube, respectively, were observed better induction in the rooting. The second experiment focused on in vitro early flowering of Dendrobium huoshanense. 0.5-1 cm plantlets, with 2-3 leave, were embedded to the medium containing TDZ, BA, 2iP or kinetin with or without NAA for induction of in vitro early flowering. The results suggested that better induction was observed in 0.5 mg/l BA and 1 mg/l BA with 1.67 and 0.83 flower buds per treatment respectively while without containing NAA, but no induction in TDZ treatment. However, the addition of 0.5 mg/l NAA revealed that TDZ and kinetin treatments were early induced in formation of flower bud and obtained 1.17 flower buds per treatment in 5 mg/l TDZ. Interestedly, BA treatment was observed to grow lot of cluster buds in the early stage and flowering in late stag subsequently. The results were observed that 0.5 mg/l BA and 1 mg/l BA yields 0.67 and 0.5 flower buds per treatment, respectively. In intro early flowering of Dendrobium huoshanense can occasionally observed in the subculture. The phenomenon implies that Dendrobium huoshanense possesses active endogenous cytokinins and results to in vitro early flowering spontaneously. However, the addition of exogenous cytokinins can enhance in vitro early flowering of Dendrobium huoshanense. Consequently, cytokinins play a crucial role in in vitro early flowering of Dendrobium huoshanense.

霍山石斛試管內形態發生及提前開花之誘導 = Induction of in vitro morphogenesis and early flowering of Dendrobium huoshanense
李, 伯倫

霍山石斛試管內形態發生及提前開花之誘導 = Induction of in vitro morphogenesis and early flowering of Dendrobium huoshanense / 李伯倫撰 - [高雄市] : 撰者, 民100. - 55葉 ; 圖，表 ; 30公分.
參考書目：葉42-55.
癒傷組織callus

霍山石斛試管內形態發生及提前開花之誘導 = Induction of in vitro morphogenesis and early flowering of Dendrobium huoshanense
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330 $a 本論文之研究分二個部份，第一部分為霍山石斛之試管內形態發生，其材料為試管內之實生苗，切取根尖、莖節及葉等作為培植體，於含有不同比例植物生長調節劑之培養基，進行癒傷組織之誘導，以做為更進一步之試驗之材料。試驗所用之基礎培養基為1/2 MS 添加20 g/l蔗糖、1 g/l蛋白腖及4 g/l水晶洋菜。試驗之設計，乃另外添加不同濃度比例之植物生長素2,4-D及細胞分裂素TDZ，以進行形態發生之誘導。經誘導結果，僅根尖於2,4-D 1 mg/l下，可以成功誘導產生癒傷組織。而所獲得之癒傷組織以同樣之培養基繼代後，發現在無任何TDZ存在下，癒傷組織呈現鮮黃色，並得以繼續生長。而這些癒傷組織經由改放置於含NAA另外添加TDZ或BA培養基後，陸續被誘導而形成類原球體。另外，在霍山石斛發根之實驗中，以0.1 mg/l NAA、1 mg/IBA及5 mg/l IBA等處理組之誘導發根反應最佳，平均分別有4、3.6及3.6的發根數。而這些處理組其分芽生長茂盛，且根部發育良好，顯然NAA及IBA對於霍山石斛之發根具有重要之角色。第二部份為霍山石斛試管內提前開花之研究，其材料以具2-3葉，約1公分大小之幼苗為材料，於含有不同比例濃度之植物生長調節劑下，進行試管內提前開花之誘導。基礎培養基內另外添加不同濃度組合之PGR，以NAA配合TDZ、BA、2iP或kinetin。試驗結果顯示，在未添加任何NAA之試驗組中，以BA及kinetin該兩組較早被誘導花苞，其中0.5 mg/l BA平均花苞形成率為1.67個最高，其次為1 mg/l BA 有0.83個，其花苞形成情形較佳，而TDZ組則無任何誘導情形發生。然而，在添加0.5 mg/l NAA試驗組方面，以TDZ及kinetin較早被誘導花苞形成，BA處理組則先大量誘導小植株產生後，於後期陸續被誘導花苞形成。統計其平均花苞形成率，以5 mg/l TDZ 有1.17個最高，5 mg/l BA及1 mg/l BA為0.67及0.5個次之。分析其上述結果，BA在有無NAA誘導下，皆具有誘導作用。在繼代霍山石斛植株時，可由其生理反應推測，霍山石斛本身內生性細胞分裂素可能較高，故能於無生長調節劑之下，即可偶發性試管內開花。但若另外添加外生性細胞分裂素，其試管內提前開花之情形會相對增加。因此，可推測細胞分裂素扮演著試管內開花重要之因子。 The thesis was separated the two sections. To study in vitro morphogenesis of Dendrobium huoshanense is the first experiment. Basal medium contains 1/2 MS salt with vitamin, 20 g/l sucrose, 1 g/l peptone and 4 g/l Gelrite. The combination of 2,4-D with TDZ were also added for induction of callus. Root tip, leaf and stem node were harvested and placed on the medium. Callus was induced and grew well from root tip in the medium with 1 mg/l 2,4-D. Bright yellow callus was subsequently transferred to the medium containing NAA combination with TDZ or BA for induction of somatic embryogenesis. The PLB were induced and regenerated after 3 months. The rooting experiment was subsequently induced by added NAA or IBA. The results revealed that 0.1 mg/l NAA, 1 mg/l IBA and 5 mg/l IBA, with 4, 3.6 and 3.6 roots per tube, respectively, were observed better induction in the rooting. The second experiment focused on in vitro early flowering of Dendrobium huoshanense. 0.5-1 cm plantlets, with 2-3 leave, were embedded to the medium containing TDZ, BA, 2iP or kinetin with or without NAA for induction of in vitro early flowering. The results suggested that better induction was observed in 0.5 mg/l BA and 1 mg/l BA with 1.67 and 0.83 flower buds per treatment respectively while without containing NAA, but no induction in TDZ treatment. However, the addition of 0.5 mg/l NAA revealed that TDZ and kinetin treatments were early induced in formation of flower bud and obtained 1.17 flower buds per treatment in 5 mg/l TDZ. Interestedly, BA treatment was observed to grow lot of cluster buds in the early stage and flowering in late stag subsequently. The results were observed that 0.5 mg/l BA and 1 mg/l BA yields 0.67 and 0.5 flower buds per treatment, respectively. In intro early flowering of Dendrobium huoshanense can occasionally observed in the subculture. The phenomenon implies that Dendrobium huoshanense possesses active endogenous cytokinins and results to in vitro early flowering spontaneously. However, the addition of exogenous cytokinins can enhance in vitro early flowering of Dendrobium huoshanense. Consequently, cytokinins play a crucial role in in vitro early flowering of Dendrobium huoshanense.
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