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美麗海葵 HRS 蛋白在胞內共生所扮演的角色之研究 = Involvem...

國立高雄大學生物科技研究所

美麗海葵 HRS 蛋白在胞內共生所扮演的角色之研究 = Involvement of ApHRS in Aiptasia-symbiodinium endosymbiosis

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Involvement of ApHRS in Aiptasia-symbiodinium endosymbiosis
作者:	黃柏翔,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	民100
面頁冊數:	100葉部份彩圖，表格 : 30公分;
標題:	胞內共生
標題:	Endosymbiosis
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/37447696527867855883
附註:	參考書目：葉74-87
摘要註:	海洋刺胞動物與胞內共生之渦鞭毛藻建立出豐腴和多樣性的珊瑚礁生態系。然而，我們對於此共生關係及參與其中的分子機制卻不是很清楚。文獻指出，哺乳類HRS (hepatocyte growth factor-regulated tyrosine kinase substrate) 參與了細胞內蛋白質運輸的調控，並且可能參與了自噬小體成熟化 (autophagosome maturation) 的過程。本研究因此希望探討 HRS蛋白在海洋刺胞動物與渦鞭毛藻胞內共生參與的情形。免疫螢光染色分析的結果顯示，大多數美麗海葵宿主細胞內的共生小體含有ApHRS免疫螢光訊號。以DCMU ( 3- ( 3,4-dichlorophenyl ) -1,1-dimethylurea ) 光合作用抑制劑處理海葵，導致ApHRS + 共生小體的比例大幅減少。吞噬作用 (phagocytosis test) 結果顯示，包著酵母菌的胞噬小體 (phagosome) 上帶有ApHRS免疫螢光訊號，而餵食120分鐘之後的胞噬小體，此比例高於餵食60分鐘之後的。進一步選殖了美麗海葵同源性蛋白LC3 (microtubule-associated protein 1 light chain 3，自噬小體標記蛋白)。在胺基酸序列上，ApLC3與人類LC3有72 %的相似性。二重免疫染色實驗發現，ApHRS和ApLC3常同時座落在相同的共生小體微膜區 (symbiosome membrane microdomains) 上。根據以上實驗結果，ApHRS和ApLC3很可能參與美麗海葵和共生藻所建立胞內共生的調控。 The intracellular association of symbiotic dinoflagellates (zooxanthellae) with marine cnidarians is the foundation of the highly productive and diversified coral reef ecosystems. However, the responsible molecular mechanism for its establishment and maintenance is still poorly known. Literature shows that HRS regulates intracellular protein sorting and may play an important role in autophagosomal maturation. The current study aimed to investigate the role of HRS protein in the endosymbiosis between the sea anemone Aiptasia pulchella and zooxanthellae. Immunostaining analysis showed that ApHRS immunoreactivities were associated with the majority of symbiosomes. DCMU treatment of the sea anemone resulted in great reduction in the percentage of ApHRS + symbiosomes within 30 min. However, this percentage rose again when DCMU treatment was prolonged to 120 min. Phagocytosis assay showed that phagosomes containing yeasts were highly decorated with ApHRS-positive immunosignals, with higher preference toward the 120 minutes-old phagosomes than 60 minutes-old phagosomes. A partial cDNA for ApLC3 protein, the sea anemone homologue of LC3 proteins, which is 72 % identical to human LC3 protein, was cloned and employed as an autophagosome marker. Double immunostaining showed that ApLC3 was located to ApHRS-positive symbiosome membrane. Overall, the present study supported that ApHRS is involved in the regulation of Aiptaisia-microalgal endosymbiosis.

美麗海葵 HRS 蛋白在胞內共生所扮演的角色之研究 = Involvement of ApHRS in Aiptasia-symbiodinium endosymbiosis
黃, 柏翔

美麗海葵 HRS 蛋白在胞內共生所扮演的角色之研究 = Involvement of ApHRS in Aiptasia-symbiodinium endosymbiosis / 黃柏翔撰 - [高雄市] : 撰者, 民100. - 100葉 ; 部份彩圖，表格 ; 30公分.
參考書目：葉74-87.
胞內共生Endosymbiosis

美麗海葵 HRS 蛋白在胞內共生所扮演的角色之研究 = Involvement of ApHRS in Aiptasia-symbiodinium endosymbiosis
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