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丹參葉培植體試管內形態發生及多倍體之誘導 = In Vitro Morp...

國立高雄大學生物科技研究所

丹參葉培植體試管內形態發生及多倍體之誘導 = In Vitro Morphogenesis from Leaf Explants and Induction of Polyploids of Salvia miliorrhiza Bunge.

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	In Vitro Morphogenesis from Leaf Explants and Induction of Polyploids of Salvia miliorrhiza Bunge.
作者:	蔡岡倫,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	民100
面頁冊數:	65葉彩圖，表格 : 30公分;
標題:	癒傷組織
標題:	callus
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/77605644275575748177
附註:	參考書目：葉50-58
摘要註:	本試驗以丹參葉片作為培植體，培養於含不同濃度的植物生長調節劑（PGR）的改良式MS培養基。結果顯示，在PGR-free的對照組，培養四週後從葉緣長出根。葉培植體培養在添加不同濃度TDZ的培養基四週後，從葉緣及葉柄傷口處長出芽體。此外，其他濃度的2,4-D及TDZ的處理，在三到四週後長出不同程度的癒傷組織。在形成芽體的處理中，濃度0.5 mg/l TDZ的培養基誘導最多芽體，平均每個葉培植體有44.4個芽體。將其所誘導的芽體移至發根培養基，可繼續發育為完整植株。在形成癒傷組織的處理中，濃度 5 mg/l 2,4-D + 0.1 mg/l TDZ之處理其癒傷組織增殖率最高，平均達27.73倍。將各癒傷組織品系置入含0、0.1、1 mg/l TDZ之培養基觀察其試管內形態發生，結果顯示，置於PGR-free的各癒傷組織品系生成不定根。置於TDZ培養基之癒傷組織則會生成叢生芽，其中以10.0 mg/l 2,4-D + 1.0 mg/l TDZ品系的癒傷組織置於1.0 mg/l TDZ的MS培養基生成最多不定芽，平均每個培植體生成28.4個芽體。在BA與NAA對癒傷組織形態發生的影響方面，NAA濃度越高則癒傷組織傾向發根、BA濃度越高則傾向生成不定芽。癒傷組織所誘導的不定芽其最佳發根培養基是0.25 mg/l BA+ 0.25 mg/l IBA的培養基，平均發根數為每培植體13.0條根。秋水仙鹼誘導葉培植體發芽方面，以原濃度2 mg/l秋水仙鹼+ 0.5 mg/l TDZ的培養基處理組芽體數最多，平均每片葉培植體有10.33個芽體。在誘導多倍體方面，濃度0.5 mg/l ~ 2 mg/l的秋水仙鹼處理組皆各誘導出一株四倍體。 Leaves of Salvia miltiorrhiza were used as explants to culture on MS medium with different concentrations of plant growth regulators. The results showed that shoots could be induced by 0.1-1 mg/l of TDZ. The treatment of 0.5 mg/l TDZ promoted shoot induction significantly, with 44.4 shoots per explants respectively. Callus could be induced by 1-10 mg/l of 2,4-D + 0.1-1 mg/l of TDZ. Callus cultured on MS medium with 5 mg/l 2,4-D + 0.1 mg/l TDZ had highest proliferation rate, with 27.73 folds respectively. Calluses were cultured on 0-1 mg/l of TDZ to observe in vitro morphogenesis of S. miltiorrhiza. Callus line of 10.0 mg/l 2,4-D + 1.0 mg/l TDZ cultured on MS medium with 1.0 mg/l TDZ induce adventitious shoots effectively, with 28.4 shoots per explants respectively. The results suggest that higher concentration of TDZ favored induction of adventitious shoot formation. Calluses were also treated with different ratio of BA and NAA. The results showed that higher NAA/BA ratio favored root induction, and lower NAA/BA ratio favored adventitious shoot induction. The optical rooting medium for callus-induced shoots was 0.25 mg/l BA+ 0.25 mg/l IBA. 0.5-4 mg/l of Colchicine were used to induce polyploidy of S. miltiorrhiza. Colchicine inhibited morphogenesis of leaf explants of S. miltiorrhiza significantly. Leaf explants cultured on 2 mg/l colchicine + 0.5 mg/l TDZ had highest number of regenerated shoots per explants. Flow cytometry were used to determine polyploidys. The result shows that leaf explants treated with 0.5, 1.0, and 2.0 mg/l colchicine + 0.5 mg/l TDZ each gain one tetraploid plantlet respectively.

丹參葉培植體試管內形態發生及多倍體之誘導 = In Vitro Morphogenesis from Leaf Explants and Induction of Polyploids of Salvia miliorrhiza Bunge.
蔡, 岡倫

丹參葉培植體試管內形態發生及多倍體之誘導 = In Vitro Morphogenesis from Leaf Explants and Induction of Polyploids of Salvia miliorrhiza Bunge. / 蔡岡倫撰 - [高雄市] : 撰者, 民100. - 65葉 ; 彩圖，表格 ; 30公分.
參考書目：葉50-58.
癒傷組織callus

丹參葉培植體試管內形態發生及多倍體之誘導 = In Vitro Morphogenesis from Leaf Explants and Induction of Polyploids of Salvia miliorrhiza Bunge.
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330 $a 本試驗以丹參葉片作為培植體，培養於含不同濃度的植物生長調節劑（PGR）的改良式MS培養基。結果顯示，在PGR-free的對照組，培養四週後從葉緣長出根。葉培植體培養在添加不同濃度TDZ的培養基四週後，從葉緣及葉柄傷口處長出芽體。此外，其他濃度的2,4-D及TDZ的處理，在三到四週後長出不同程度的癒傷組織。在形成芽體的處理中，濃度0.5 mg/l TDZ的培養基誘導最多芽體，平均每個葉培植體有44.4個芽體。將其所誘導的芽體移至發根培養基，可繼續發育為完整植株。在形成癒傷組織的處理中，濃度 5 mg/l 2,4-D + 0.1 mg/l TDZ之處理其癒傷組織增殖率最高，平均達27.73倍。將各癒傷組織品系置入含0、0.1、1 mg/l TDZ之培養基觀察其試管內形態發生，結果顯示，置於PGR-free的各癒傷組織品系生成不定根。置於TDZ培養基之癒傷組織則會生成叢生芽，其中以10.0 mg/l 2,4-D + 1.0 mg/l TDZ品系的癒傷組織置於1.0 mg/l TDZ的MS培養基生成最多不定芽，平均每個培植體生成28.4個芽體。在BA與NAA對癒傷組織形態發生的影響方面，NAA濃度越高則癒傷組織傾向發根、BA濃度越高則傾向生成不定芽。癒傷組織所誘導的不定芽其最佳發根培養基是0.25 mg/l BA+ 0.25 mg/l IBA的培養基，平均發根數為每培植體13.0條根。秋水仙鹼誘導葉培植體發芽方面，以原濃度2 mg/l秋水仙鹼+ 0.5 mg/l TDZ的培養基處理組芽體數最多，平均每片葉培植體有10.33個芽體。在誘導多倍體方面，濃度0.5 mg/l ~ 2 mg/l的秋水仙鹼處理組皆各誘導出一株四倍體。 Leaves of Salvia miltiorrhiza were used as explants to culture on MS medium with different concentrations of plant growth regulators. The results showed that shoots could be induced by 0.1-1 mg/l of TDZ. The treatment of 0.5 mg/l TDZ promoted shoot induction significantly, with 44.4 shoots per explants respectively. Callus could be induced by 1-10 mg/l of 2,4-D + 0.1-1 mg/l of TDZ. Callus cultured on MS medium with 5 mg/l 2,4-D + 0.1 mg/l TDZ had highest proliferation rate, with 27.73 folds respectively. Calluses were cultured on 0-1 mg/l of TDZ to observe in vitro morphogenesis of S. miltiorrhiza. Callus line of 10.0 mg/l 2,4-D + 1.0 mg/l TDZ cultured on MS medium with 1.0 mg/l TDZ induce adventitious shoots effectively, with 28.4 shoots per explants respectively. The results suggest that higher concentration of TDZ favored induction of adventitious shoot formation. Calluses were also treated with different ratio of BA and NAA. The results showed that higher NAA/BA ratio favored root induction, and lower NAA/BA ratio favored adventitious shoot induction. The optical rooting medium for callus-induced shoots was 0.25 mg/l BA+ 0.25 mg/l IBA. 0.5-4 mg/l of Colchicine were used to induce polyploidy of S. miltiorrhiza. Colchicine inhibited morphogenesis of leaf explants of S. miltiorrhiza significantly. Leaf explants cultured on 2 mg/l colchicine + 0.5 mg/l TDZ had highest number of regenerated shoots per explants. Flow cytometry were used to determine polyploidys. The result shows that leaf explants treated with 0.5, 1.0, and 2.0 mg/l colchicine + 0.5 mg/l TDZ each gain one tetraploid plantlet respectively.
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610 1 $a callus $a morphogenesis $a polyploidy $a tissue culture $a Salvia miltiorrhiza
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