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Topological bonding of DNA nanostruc...

New York University.

Topological bonding of DNA nanostructures.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Topological bonding of DNA nanostructures.
Author:	Ohayon, Yoel.
Description:	846 p.
Notes:	Source: Dissertation Abstracts International, Volume: 72-06, Section: B, page: .
Notes:	Adviser: Nadrian C. Seeman.
Contained By:	Dissertation Abstracts International72-06B.
Subject:	Chemistry, Biochemistry.
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3445320
ISBN:	9781124544755

Topological bonding of DNA nanostructures.
Ohayon, Yoel.

Topological bonding of DNA nanostructures. - 846 p.

Source: Dissertation Abstracts International, Volume: 72-06, Section: B, page: .

Thesis (Ph.D.)--New York University, 2011.

The properties of DNA that allow it to act as the storage medium of genetic information also make it an outstanding molecule for use in nanotechnology. This fact has led to the development of DNA nanotechnology which is based on Watson-Crick base pairing, but the backbone structures involved are complex species, not simple linear duplex molecules. These motifs and various programmable structures in one, two and three dimensions are constructed by using sticky-ends which are short-linear extensions that hold complementary structures together.

ISBN: 9781124544755Subjects--Topical Terms:

226900
Chemistry, Biochemistry.

Topological bonding of DNA nanostructures.
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100 1 $a Ohayon, Yoel. $3 530999
245 1 0 $a Topological bonding of DNA nanostructures.
300 $a 846 p.
500 $a Source: Dissertation Abstracts International, Volume: 72-06, Section: B, page: .
500 $a Adviser: Nadrian C. Seeman.
502 $a Thesis (Ph.D.)--New York University, 2011.
520 $a The properties of DNA that allow it to act as the storage medium of genetic information also make it an outstanding molecule for use in nanotechnology. This fact has led to the development of DNA nanotechnology which is based on Watson-Crick base pairing, but the backbone structures involved are complex species, not simple linear duplex molecules. These motifs and various programmable structures in one, two and three dimensions are constructed by using sticky-ends which are short-linear extensions that hold complementary structures together.
520 $a The PX (Paranemic Crossover) motif has also been reported as another form of cohesion for large DNA structures to overcome some of the shortcomings of sticky-ended cohesion to hold together such structures. Both the sticky-ended and the PX cohesion are based on hydrogen-bonded interactions which cannot withstand denaturing conditions such as chemical denaturants and elevated temperatures.
520 $a In the projects presented, we demonstrate that the hydrogen-bonded cohesion of DNA nanostructures can be transformed into a topological interaction. In the first chapter, we converted the PX interaction of DNA circles containing cohesive loops into catenated structures via the use of Topo I enzyme by creating a linkage between the loops.
520 $a In the second chapter, we created a topological interaction between DNA structures that cohere via both PX and sticky-ends. Covalent linkages were created between the functionalized 3' and 5' ends of the sticky-ends. The torus-like structures were obtained via enzymatic, chemical and non-enzymatic methods. A DNA knot was also created by PX cohering structures containing both cohesive loops and sticky-end interaction.
520 $a The methods developed in the first two chapters were used to construct topologically linked one-dimensional DNA arrays assembled from different types of PX cohering tiles. The construction of these poly-catenated scaffolds allowed for a new method to position nano-particles on linear structures.
520 $a In the last chapter, an attempt was made to assemble a catenated two-dimensional DNA array assembled from circular cross-like DNA structures that cohere via paranemic interactions.
590 $a School code: 0146.
650 4 $a Chemistry, Biochemistry. $3 226900
650 4 $a Nanotechnology. $3 193873
690 $a 0487
690 $a 0652
710 2 $a New York University. $b Chemistry. $3 531000
773 0 $t Dissertation Abstracts International $g 72-06B.
790 1 0 $a Seeman, Nadrian C., $e advisor
790 1 0 $a Seeman, Nadrian C. $e committee member
790 1 0 $a Canary, James $e committee member
790 1 0 $a Vologodskii, Alexander $e committee member
790 $a 0146
791 $a Ph.D.
792 $a 2011
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3445320