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脂質代謝影響蝴蝶蘭抽梗的研究 = Studies of the effe...

國立高雄大學生物科技研究所

脂質代謝影響蝴蝶蘭抽梗的研究 = Studies of the effects of lipid metabolism on the spike emergence in Phalaenopsis aphrodite subsp. formosana.

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Studies of the effects of lipid metabolism on the spike emergence in Phalaenopsis aphrodite subsp. formosana.
作者:	許懿璿,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	2012[民101]
面頁冊數:	112面圖，表格 : 30公分;
標題:	葉綠體蛋白質
標題:	Chloroplast proteins
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/65212040877669592663
附註:	參考書目：面76-92
摘要註:	溫度與光照是誘導蝴蝶蘭花梗抽出的重要環境因子。本研究將成熟的台灣阿嬤蝴蝶蘭（Phalaenopsis aphrodite subsp. formosana）培養於生長箱9-12天，分別於日/夜溫為28/20℃，照度為150 μmole m-2s-1的正常抽梗處理，以及同上述條件但夜溫為28℃的加溫或照度為20 μmole m-2s-1的遮蔭抑梗處理，探討台灣阿嬤在不同條件下，不同葉齡，包括第一位葉（新形成且未完全展開葉），第二位葉（新形成完全展開葉）及第五位葉（老葉）脂質相關的變化，期望了解蝴蝶蘭脂質代謝與抽梗的相關性。此外，透過建立一個快速且有效率分離第二位葉完整葉綠體的方法，探討加溫與遮蔭對葉綠體脂質代謝及蛋白質體表現的影響。實驗結果顯示，遮蔭處理會減少脂質含量，包括第一、五位葉monogalactosyldiacylglycerol（MGDG）和digalactosyldiacylglycerol（DGDG）、第二位葉的malondialdehyde（MDA）、各位葉的tocopherol含量，以及色素化合物，包括各位葉葉綠素b、第二與第五位葉carotenoids的含量，推論遮蔭處理抑梗可能與降低光合效率及影響脂質代謝有關。雖然加溫處理會增加第五位葉tocopherol含量及減少第二位葉DGDG含量，但MDA、葉綠素a、葉綠素b含量與正常處理皆無顯著差異，推測加溫處理抑梗機制不同於遮蔭處理。此外，不論任何處理的tocopherol及許多未知脂質化合物於第五位葉的含量皆有較多的趨勢，其成份和功能有待進一步釐清。完整的葉綠體累積於Percoll濃度梯度40–80% 之間，且三種處理得到完整葉綠體的比率為60-70%。蝴蝶蘭的葉綠體內並不存在tocopherol，且遮蔭處理所分離出葉綠體的總脂質、MGDG 和DGDG含量皆高於正常與加溫處理。蛋白質體分析顯示，遮蔭處理的葉綠體蛋白質與正常和加溫處理的略有不同，但大部分的蛋白質為光合系統II（photosystem II-related proteins）與ATP合成相關的蛋白質。整體而言，台灣阿嬤蝴蝶蘭在不同處理下葉綠體的變化與抽梗之間的關係仍需要進一步的探討。 Temperature and light are important environmental factors that influence flower stalk development in Phalaenopsis. In this study, the mature Phalaenopsis aphrodite subsp. formosana grown on 28℃ day/20℃ night and 150 μmole m-2s-1 photon irradiance for 9-12 days was normal for spiking induction treatment, and the same as the above conditions except 28℃ night temperature was warm or 20 μmole m-2s-1 photon irradiance was shade for spiking inhibition treatments. In order to understand the relationship between the lipid metabolism and flower stalk development of Phalaenopsis, this study examined the lipid related variation in the leaves of three different ages, including the first leaf (a newly and not fully expanded leaf), the second leaf (a newly and fully expanded leaf) and the fifth leaf (the old leaf) from Phalaenopsis exposed to different treatments. Additionally, a rapid and efficient method to isolate the intact chloroplast from the second leaf was established, to explore the impact of warm and shade treatments on the lipid metabolism and proteomic expression of the chloroplast.The results showed that shade treatment reduced the amounts of lipid, including monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) in the first and fifth leaves, malondialdehyde (MDA) in the second leaf and tocopherol in all leaves, and pigments such as chlorophyll b in all leaves, carotenoids in the second and fifth leaves. These results disclose that the reduction of photosynthetic efficiency and the increase of lipid degradation may be related with shade treatment inhibited spike emergence. Warm treatment increased the content of tocopherol in the fifth leaf and decreased the content of DGDG in the second leaf, but the contents of MDA, chlorophyll a, chlorophyll b did not show differences between normal and warm treatments, implying that the mechanism of spiking inhibition by warm treatment is different to shade treatment. In addition, there was a consistent tendency about higher amounts of tocopherol and a lot of unknown lipid compounds exhibited in the fifth leaf of all treatments, their compositions and functions still need to be clarified.The intact chloroplast formed a band at the 40–80% interface of Percoll layer gradients, and their intact percentages were 60-70% for all treatments. Tocopherol did not exist in the intact chloroplasts. The amounts of total lipid, MGDG and DGDG in the shaded chloroplasts were all significantly higher than those in the chloroplasts isolated from warm and normal treatments. Proteomic analysis showed that the proteins in the shaded chloroplast was slightly different from those of chloroplasts isolated from warm and normal treatments, but the abundant proteins resulting from all the treatments were identified as photosystem II-related and ATP synthesis-related proteins. On the whole, the relationship between the alteration of the chloroplast and flower stalk development of P. aphrodite under various conditions remains to be investigated.

脂質代謝影響蝴蝶蘭抽梗的研究 = Studies of the effects of lipid metabolism on the spike emergence in Phalaenopsis aphrodite subsp. formosana.
許, 懿璿

脂質代謝影響蝴蝶蘭抽梗的研究 = Studies of the effects of lipid metabolism on the spike emergence in Phalaenopsis aphrodite subsp. formosana. / 許懿璿撰 - [高雄市] : 撰者, 2012[民101]. - 112面 ; 圖，表格 ; 30公分.
參考書目：面76-92.
葉綠體蛋白質Chloroplast proteins

脂質代謝影響蝴蝶蘭抽梗的研究 = Studies of the effects of lipid metabolism on the spike emergence in Phalaenopsis aphrodite subsp. formosana.
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330 $a 溫度與光照是誘導蝴蝶蘭花梗抽出的重要環境因子。本研究將成熟的台灣阿嬤蝴蝶蘭（Phalaenopsis aphrodite subsp. formosana）培養於生長箱9-12天，分別於日/夜溫為28/20℃，照度為150 μmole m-2s-1的正常抽梗處理，以及同上述條件但夜溫為28℃的加溫或照度為20 μmole m-2s-1的遮蔭抑梗處理，探討台灣阿嬤在不同條件下，不同葉齡，包括第一位葉（新形成且未完全展開葉），第二位葉（新形成完全展開葉）及第五位葉（老葉）脂質相關的變化，期望了解蝴蝶蘭脂質代謝與抽梗的相關性。此外，透過建立一個快速且有效率分離第二位葉完整葉綠體的方法，探討加溫與遮蔭對葉綠體脂質代謝及蛋白質體表現的影響。實驗結果顯示，遮蔭處理會減少脂質含量，包括第一、五位葉monogalactosyldiacylglycerol（MGDG）和digalactosyldiacylglycerol（DGDG）、第二位葉的malondialdehyde（MDA）、各位葉的tocopherol含量，以及色素化合物，包括各位葉葉綠素b、第二與第五位葉carotenoids的含量，推論遮蔭處理抑梗可能與降低光合效率及影響脂質代謝有關。雖然加溫處理會增加第五位葉tocopherol含量及減少第二位葉DGDG含量，但MDA、葉綠素a、葉綠素b含量與正常處理皆無顯著差異，推測加溫處理抑梗機制不同於遮蔭處理。此外，不論任何處理的tocopherol及許多未知脂質化合物於第五位葉的含量皆有較多的趨勢，其成份和功能有待進一步釐清。完整的葉綠體累積於Percoll濃度梯度40–80% 之間，且三種處理得到完整葉綠體的比率為60-70%。蝴蝶蘭的葉綠體內並不存在tocopherol，且遮蔭處理所分離出葉綠體的總脂質、MGDG 和DGDG含量皆高於正常與加溫處理。蛋白質體分析顯示，遮蔭處理的葉綠體蛋白質與正常和加溫處理的略有不同，但大部分的蛋白質為光合系統II（photosystem II-related proteins）與ATP合成相關的蛋白質。整體而言，台灣阿嬤蝴蝶蘭在不同處理下葉綠體的變化與抽梗之間的關係仍需要進一步的探討。 Temperature and light are important environmental factors that influence flower stalk development in Phalaenopsis. In this study, the mature Phalaenopsis aphrodite subsp. formosana grown on 28℃ day/20℃ night and 150 μmole m-2s-1 photon irradiance for 9-12 days was normal for spiking induction treatment, and the same as the above conditions except 28℃ night temperature was warm or 20 μmole m-2s-1 photon irradiance was shade for spiking inhibition treatments. In order to understand the relationship between the lipid metabolism and flower stalk development of Phalaenopsis, this study examined the lipid related variation in the leaves of three different ages, including the first leaf (a newly and not fully expanded leaf), the second leaf (a newly and fully expanded leaf) and the fifth leaf (the old leaf) from Phalaenopsis exposed to different treatments. Additionally, a rapid and efficient method to isolate the intact chloroplast from the second leaf was established, to explore the impact of warm and shade treatments on the lipid metabolism and proteomic expression of the chloroplast.The results showed that shade treatment reduced the amounts of lipid, including monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) in the first and fifth leaves, malondialdehyde (MDA) in the second leaf and tocopherol in all leaves, and pigments such as chlorophyll b in all leaves, carotenoids in the second and fifth leaves. These results disclose that the reduction of photosynthetic efficiency and the increase of lipid degradation may be related with shade treatment inhibited spike emergence. Warm treatment increased the content of tocopherol in the fifth leaf and decreased the content of DGDG in the second leaf, but the contents of MDA, chlorophyll a, chlorophyll b did not show differences between normal and warm treatments, implying that the mechanism of spiking inhibition by warm treatment is different to shade treatment. In addition, there was a consistent tendency about higher amounts of tocopherol and a lot of unknown lipid compounds exhibited in the fifth leaf of all treatments, their compositions and functions still need to be clarified.The intact chloroplast formed a band at the 40–80% interface of Percoll layer gradients, and their intact percentages were 60-70% for all treatments. Tocopherol did not exist in the intact chloroplasts. The amounts of total lipid, MGDG and DGDG in the shaded chloroplasts were all significantly higher than those in the chloroplasts isolated from warm and normal treatments. Proteomic analysis showed that the proteins in the shaded chloroplast was slightly different from those of chloroplasts isolated from warm and normal treatments, but the abundant proteins resulting from all the treatments were identified as photosystem II-related and ATP synthesis-related proteins. On the whole, the relationship between the alteration of the chloroplast and flower stalk development of P. aphrodite under various conditions remains to be investigated.
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