登革熱病媒蚊綜合防治研究 = The integrated vector...
國立高雄大學運動健康與休閒學系碩士班

 

  • 登革熱病媒蚊綜合防治研究 = The integrated vector management of dengue fever
  • 紀錄類型: 書目-語言資料,印刷品 : 單行本
    並列題名: The integrated vector management of dengue fever
    作者: 曾朝豐,
    其他團體作者: 國立高雄大學
    出版地: [高雄市]
    出版者: 撰者;
    出版年: 2012[民101]
    面頁冊數: 95面圖,表格 : 30公分;
    標題: 登革熱病媒蚊
    標題: victor of dengue fever
    電子資源: http://handle.ncl.edu.tw/11296/ndltd/49008423115528785715
    附註: 參考書目:面35-40
    摘要註: 登革熱防治必須因地、因時制宜進行綜合防治才能獲得良好防治成果,本研究探討病媒蚊抗藥性,進行綠籬成蟲防治試驗,使用對人體無毒性且環境無害之製劑如蘇力菌(Bacillus thuringiensis var. israelensis H-14型)、賜諾殺(spinosad)、昆蟲生長調節劑百利普芬(pyriproxyfen)及單分子膜(AMF),進行實驗室登革熱病媒蚊之感受性試驗及模擬試驗。研究發現埃及斑蚊對有機磷類殺蟲劑均無抗藥性(24小時成蟲死亡率均為100%)。在綠籬防治試驗中,陽性率及蟲卵數在第滅寧噴藥後1星期防治率分別達83.9及95.5%,可有效防治病媒蚊;持續3星期,防治效果分別為60.1%及78.1%。幼蟲防治研究發現登革熱病媒蚊幼蟲對蘇力菌及賜諾殺皆無抗藥性,蘇力菌、賜諾殺、百利普芬對埃及斑蚊感性品系(Bora Bora)之半數致死濃度(LC50)分別為44.9 ppb、4.7 ppb及0.019 ppb,僅需微量濃度便可有效殺滅埃及斑蚊感性品系之幼蟲。使用濃度1 ml/m2單分子膜對登革熱病媒蚊幼蟲防治72小時死亡率達100%;對蚊蛹之半數致死時間(LT50)為160分鐘,蚊蛹全數殺滅時間為210分鐘。以百利普芬及蘇力菌(0.001:4)混合液測試,混合液對埃及斑蚊感性品系之LC50為0.354 ppb;百利普芬及賜諾殺(0.01:5)混合液測試,對埃及斑蚊感性品系之LC50為0.054 ppb,進行combination index(CI)分析,顯示上述混合液對登革熱病媒蚊幼蟲之殺滅有相乘作用(synergism effect)。在模擬試驗中,以濃度10及50 ppb之百利普芬經室內、外模擬試驗結果,發現持續9星期仍有100%之幼蟲無法羽化為成蟲;以濃度50及100 ppb之賜諾殺經室內模擬試驗結果,則持續有4及5星期之藥效;依據模擬試驗結果,以5 ppb百利普芬加上50 ppb蘇力菌或賜諾殺進行登革熱病媒蚊綜合防治,可達24小時100%病媒蚊幼蟲致死率,且室外病媒蚊幼蟲防治後4週仍可維持100%之殘效效果,可作為綜合防治應用之參考。Dengue fever prevention and control can not relying on a single method to achieve successful control. In this study, the insecticides resistance of the mosquito was investigated, green barrier spray to control adult mosquito test was conducted, meanwhile, the environment friendly pesticides such as Bacillus thuringiensis var. israelensis H-14 (Bti), spinosad, insect growth regulator pyriproxyfen and monomolecule film (AMF) were used to test for the Dengue fever vectors. The results were shown that Aedes aegypti without insecticides resistance to organophosphorus insecticides. Green barrier spray method can effectively reduce the ovitrap indices and eggs number of mosquito to achieve good prevention effect. The results of the larva prevention study found that the larva of Dengue fever vectors did not show insecticides resistance to Bti and spinosad. It was found that the 50% Lethal Concentrations (LC50) of Bti, spinosad and pyriproxyfen to Bora Bora of A. aegypti were respectively 44.9, 4.7 and 0.019 ppb, that is, very low concentration to control the larva of the Bora Bora of A. aegypti effectively. AMF can kill the larva within 72 hours (mortality 100%), and the 50% Lethal Time (LT50) to the pupa of mosquito is 160 minutes of concentration in 1 ml/m2. All pupae were killed until 210 minture. The mixed reagent of pyriproxyfen and Bti (0.001: 4) was used for the test, the mixed solution showed LC50 of 0.354 ppb to Bora Bora of A. aegypti. The mixed solution of pyriproxyfen and spinosad (0.01: 5) was used for the test, the mixed solution showed a LC50 of 0.054 ppb to Bora Bora of A. aegypti. In addition, results of further combination index analysis showed synergism effects of these testing mixtures. Moreover, the residual effects of pyriproxyfen laboratory simulation test (10 and 50 ppb in indoor and outdoor) were evaluated once a week for 9 weeks and no mosquito larvae were found. The results of spinosad laboratory simulation tests (50 and 100 ppb in indoor) were found to have 100% killing effect 4 weeks and 5 weeks after indoor application, respectively. The 24 hours mortality of dengue vector larvae were 100% by mixed reagent (5 ppb pyriproxyfen and 100 ppb Bti or 50 ppb spinosad), and the residual effects of mixed reagent was 4 weeks. The results may be used as a reference for the government in establishing strategies on the integrated vector management of dengue.
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310002295379 博碩士論文區(二樓) 不外借資料 學位論文 TH 008M/0019 896219 8042 2012 一般使用(Normal) 在架 0
310002295387 博碩士論文區(二樓) 不外借資料 學位論文 TH 008M/0019 896219 8042 2012 c.2 一般使用(Normal) 在架 0
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