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光質對蝴蝶蘭中苗抽梗之研究 = Studies on the effec...

呂俊賢

光質對蝴蝶蘭中苗抽梗之研究 = Studies on the effects of light quality on the spike emergence of Phalaenopsis young plants

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Studies on the effects of light quality on the spike emergence of Phalaenopsis young plants
作者:	呂俊賢,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	2013[民102]
面頁冊數:	118面圖，表格 : 30公分;
標題:	抗氧化
標題:	antioxidation
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/89820003285905284558
附註:	107年4月10日公開
附註:	參考書目：面80-97
摘要註:	光質會影響植物的生長發育。控制蝴蝶蘭(Phalaenopsis spp.)抽梗是調控花期的關鍵步驟之一。本論文探討不同光質對生長在2.5 吋盆1 個月的台灣阿嬤（Phalaenopsis aphrodite subsp. formosana）中苗抽梗，以及生理生化特性的影響。中苗培養在生長箱內，設定日/夜溫28/20 ℃，日/夜光週期14/10 小時，以及約70 μmole m-2s-1 photosynthetically active radiation（PAR）光照強度且具有三種不同光質條件：（1）100%螢光燈管（tube fluorescent lamp, TFL）的控制組（W），（2）90 % TFL 補充10 %發光二極體（light-emitting diode, LED）提供的藍光處理（WB）或（3）紅光處理（WR）。研究結果顯示，在三種處理六週後，中苗的生長、氣孔開啟比率與不同的色素濃度皆未有顯著差異。然而，WB 蘭苗的葉背具有最高的氣孔密度，以及WR 蘭苗呈現最高的花梗抽出比率，顯示不同光質會影響中苗發育。三種處理蘭苗的光化學消散（photochemical quenching, qp）能力與蔗糖濃度皆無顯著差異，暗示蝴蝶蘭抽梗與蔗糖濃度無關。經時的研究結果顯示，蘭苗於WR 處理三週內的Cu-Zn superoxide dimutase （SOD）及Mn-SOD 活性高於W 處理，至第六週則hydrogen peroxide 與還原態維他命C 濃度，以及可溶性ascorbate peroxidase（APX）活性高於W 處理。二維電泳與即時聚合酶連鎖反應分析進一步證實WR 處理誘導蘭苗表現較高的APX 蛋白質及基因。因此，稍微補充紅光增加抗氧化表現可能參與蝴蝶蘭中苗花梗的萌發。 Light quality influences plant growth and development. One of the key steps in regulating Phalaenopsis flowering is controlling spike emergence. This thesis studied the effects of different light qualities on the spike emergence, physiological and biochemical attributes of young Phalaenopsis aphrodite subsp. formosana plants transferred into 2.5-inch diameter white plastic pots for one month. The young plants were grown in a growth chamber with day/night temperatures of 28/20 °C and a day/night photoperiod of 14/10 hours with 70 μmol m-2s-1 of photosynthetically active radiation under three different light quality regimes: 1) a control group given 100 % tube fluorescent lamp (TFL) (W), 2) a treatment group given 90 % TFL combined with supplemental 10 % blue light-emitting diode (LED) (WB), or 3) a treatment group given 10 % red LED (WR). The results clearly revealed that the growth, stomatal opening percentage and different pigment concentrations of the young plants did not differ significantly among the three treatments for six weeks; however, WB plants exhibited the highest stomatal density on the abaxial surface, and WR plants showed the highest spiking ratio, indicating that different light qualities affect the development of young Phalaenopsis plants. The photochemical quenching capacity (qp) and the sucrose concentration levels of the plants also did not exhibit significant differences for the three treatments, implying that there isnot a close relationship between spike emergence and sucrose concentration inPhalaenopsis. Time course studies showed that the WR plants had higher Cu-Znsuperoxide dismutase (SOD) and Mn-SOD activities within three weeks. The WR plants also had higher hydrogen peroxide and reduced ascorbic acid (AsA) concentrations, as well as increased soluble form ascorbate peroxidase (APX) activity, at the sixth week than the W plants. Two-dimensional electrophoresis and real-time PCR analysis further confirmed that the APX protein and gene were highly expressed under WR treatment. Therefore, increasing antioxidative expression by means of a slight red light supplement may possibly be involved in increasing the spike emergence of young Phalaenopsis plants.

光質對蝴蝶蘭中苗抽梗之研究 = Studies on the effects of light quality on the spike emergence of Phalaenopsis young plants
呂, 俊賢

光質對蝴蝶蘭中苗抽梗之研究 = Studies on the effects of light quality on the spike emergence of Phalaenopsis young plants / 呂俊賢撰 - [高雄市] : 撰者, 2013[民102]. - 118面 ; 圖，表格 ; 30公分.
107年4月10日公開參考書目：面80-97.
抗氧化antioxidation

光質對蝴蝶蘭中苗抽梗之研究 = Studies on the effects of light quality on the spike emergence of Phalaenopsis young plants
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