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於蝴蝶蘭中過量表現pflp基因可增強光合效率及醣類累積 = Overex...

國立高雄大學生物科技研究所

於蝴蝶蘭中過量表現pflp基因可增強光合效率及醣類累積 = Overexpressing Plant Ferredoxin-like Protein Enhances Photosynthetic Efficiency and Carbohydrates Accumulation in Phalaenopsis

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Overexpressing Plant Ferredoxin-like Protein Enhances Photosynthetic Efficiency and Carbohydrates Accumulation in Phalaenopsis
作者:	陳彥廷,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	2011[民100]
面頁冊數:	38面圖，表格 : 30公分;
標題:	植物硫鐵蛋白，光合作用，蝴蝶蘭
標題:	Plant Ferredoxin-like Protein (PFLP)
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/34787834255755078251
摘要註:	景天酸代謝為三種二氧化碳固定途徑之一，其特性為光合速率較慢但呈現極高的水分利用。雖然先前研究指出表現甜椒 plant ferredoxin-like protein (PFLP) 蛋白可增強植物對細菌性病原的抵抗力。但是由於 PFLP 其蛋白質序列與光系統I (photosystem I)中的光合蛋白 ferredoxin-1 (Fd-1) 具有高度相似性，所以如在植物中過表達該基因，有可能促使轉殖植物其光合能力增加。為了證明 PFLP 可促進光合能力，我們將 pflp 基因片段轉殖進入蝴蝶蘭，並加以分析其光合指標。在電子傳遞鏈中，轉殖植物呈現較高的電子傳遞速率，且二氧化碳吸收速率及有機酸含量亦比野生型高。在酵素活性方面，進行貯存二氧化碳形成有機酸的酵素phosphoenolpyruvate carboxylase 以及釋放二氧化碳進入卡爾文循環的酵素NAD+-linked malic enzyme 兩者活性於轉殖株較高。此外可溶性醣類如：葡萄糖、果糖及蔗糖其含量於轉殖株中較高，澱粉粒亦大量累積於轉殖株中。以上結果顯示於轉殖株中過表達 pflp 基因將會刺激電子傳遞以增強醣類累積。總而言之 pflp 不僅可增加抗病亦可促進光合作用，於農業應用上為極有效用的基因。 Crassulacean acid metabolism (CAM) is one of three major models of carbon dioxide assimilation pathway with better water-use efficiency and slower photosynthetic efficiency in photosynthesis. Previous studies indicate that expressing gene of sweet pepper plant ferredoxin-like protein (PFLP) can enhance disease resistance to bacterial pathogens. The protein sequence of PFLP shows high homology to the ferredoxin-1(Fd-1) family that belongs to photosynthetic type Fd and involves in photosystem I. It is proposed that overexpressing pflp in transgenic plant may enhance photosynthetic efficiency. To investigate whether PFLP could improve photosynthetic efficiency, pflp transgenic Phalaenopsis were generated to analyze photosynthetic markers. Transgenic plant exhibited highly electron transport rate than that of wild type (WT). Compared with WT, transgenic plant showed higher CO2 assimilation rate and organic acids contents. Both of phosphoenolpyruvate carboxylase (PEPC) and NAD+-linked malic enzyme (NAD+-ME) activities also increased in transgenic plant. Finally, the photosynthesis products were examined between transgenic plant and WT. Soluble sugars contents such as glucose, fructose, and sucrose showed significantly increased in transgenic plant. The starch grains also highly accumulated in transgenic plant than that of WT. These above results indicate that overexpressing pflp in transgenic plant increases carbohydrates accumulation by the stimulation of electron transport flow. Altogether, we suggest pflp may be an extremely useful gene for agriculture that not only increases disease resistant but also enhances photosynthetic efficiency.

於蝴蝶蘭中過量表現pflp基因可增強光合效率及醣類累積 = Overexpressing Plant Ferredoxin-like Protein Enhances Photosynthetic Efficiency and Carbohydrates Accumulation in Phalaenopsis
陳, 彥廷

於蝴蝶蘭中過量表現pflp基因可增強光合效率及醣類累積 = Overexpressing Plant Ferredoxin-like Protein Enhances Photosynthetic Efficiency and Carbohydrates Accumulation in Phalaenopsis / 陳彥廷撰 - [高雄市] : 撰者, 2011[民100]. - 38面 ; 圖，表格 ; 30公分.
參考書目：面26-31.
植物硫鐵蛋白，光合作用，蝴蝶蘭Plant Ferredoxin-like Protein (PFLP)

於蝴蝶蘭中過量表現pflp基因可增強光合效率及醣類累積 = Overexpressing Plant Ferredoxin-like Protein Enhances Photosynthetic Efficiency and Carbohydrates Accumulation in Phalaenopsis
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