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幾丁聚醣奈米線之製備及其在止血材料之應用 = The preparati...

何應強

幾丁聚醣奈米線之製備及其在止血材料之應用 = The preparation of chitosan nanowires and its application to hemostasis

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	The preparation of chitosan nanowires and its application to hemostasis
作者:	何應強,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	2013[民102]
面頁冊數:	83面圖，表格 : 30公分;
標題:	奈米線
標題:	nanowires
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/80426416498018648604
附註:	參考書目：面69-72
附註:	102年10月31日公開
摘要註:	本研究利用幾丁聚醣奈米線(chitosan nanowires)做為止血劑之材料。藉由幾丁聚醣天然之止血性質，可吸附、活化血小板，並促進纖維蛋白表現。將其製備成奈米線可提高其比表面積值，以增加幾丁聚醣與和血球細胞之作用面積。此外，透過交聯劑處理使其形成類似於纖維蛋白之網狀結構，在凝血過程中能攔截及包覆更多血小板與紅血球，以達快速止血之作用。於製程中，我們改良傳統電紡絲製程，利用材料之特性製備出有別於一般奈米纖維且較短之奈米線。其製作方法是利用醋酸沸點高，於紡絲過程中不易揮發，以及低分子量幾丁聚醣溶液，高分子間糾結情形不良，無法於紡絲過程中連續噴發的兩個特性，使其噴發出液態之奈米線，並於接地之金屬網下放置沉澱劑，固化並收集奈米線。藉由控制電紡絲之相關參數，如：沉澱劑之比例與工作電壓等，可製備出不同直徑大小之奈米線，隨著工作電壓提高可製備出直徑相對小之奈米線，而沉澱劑中氫氧化納和乙醇之比例，除了會影響奈米線之形態與減少沉澱物產生外，也會造成奈米線直徑上之變化，其直徑最小可達約10nm。此外，也透過超音破震盪破壞奈米纖維結構製備奈米線。利用幾丁聚醣和聚乙烯醇(PVA)作為電紡絲溶液，並在金屬收集板上方置放乙醇溶液收集chitosan/PVA之奈米纖維。收集到之奈米纖維利用超音波震盪處理，以15W功率持續3分鐘，成功地使造成奈米纖維結構斷裂形成較短之奈米線，且未造成結構上之破壞，於乾燥、純化後及回溶於溶液中皆可保持良好的線狀結構。在凝血測試中，經由三聚磷酸(TPP)及褐藻酸(alginate)交聯之奈米線，分別產生不同結果與現象。與TPP交聯之奈米線會促進纖維蛋白的表現，但需較長時間才能使其大量表現；與alginate交聯之奈米線則有良好之吸水性，能在短時間內吸收較大量之血液。不兩者之間相同點是交聯之結果均造成幾丁聚醣吸附、活化血小板的能力下降，其顯示幾丁聚醣之胺基(NH3+)經由交聯後大量減少。從實驗結果得知幾丁聚醣藉由正電荷吸附，細胞膜表面帶負電之紅血球及血小板，並造成血小板之變形與活化作用，進而促進纖維蛋白之產生，以達到止血效果。 We used chitosan nanowires as hemostatic materials in this research according to the hemostasis property of chitosan, which can also absorb and activate platelets and enhance the formation of fibrin. The nanowire form of chitosan was expected to display high aspect ratios and high surface areas to interact with blood cells. By treatment of crosslinker, the nanowires was fixed for their morphology and formed a network structure in order to intercept more platelets and erythrocytes to speed up hemostasis occurance. In the preparation of nanowires, we improved the traditional electro-spinning process to fabricate short nanowires by using high-boiling-pointed acetic acid and low-molecular-weighted chitosan. Due to low evaporating property of the solution and less entanglement between polymer chains, the shortened chitosan nanowires could be obtained via collecting nanowires passing through a grounded metal mesh in a basic alcoholic solution. The diameters of nanowires could be controlled via some parameters: precipitating agents, operation voltages, and working distances, and so on. Higher operation voltage generated relatively narrow nanowires. the precipitating agents using different NaOH/ethanol ratios could dominate the morphology of nanowires, affect the precipitation, and change diameters of nanowires. Furthermore, we also used chitosan/PVA to form the co-precipitated nanofibers, and then dispersed via ultrasonic treatment (15 W for 3 min) to form shortened chitosan/PVA nanowires. After drying, purifying, and re-dispersing the nanowires into PBS, the wire-like structure of nanowires could be maintained well. In blood coagulation tests, using tripolyphosphate (TPP) and alginate to crosslink the nanowire surfaces displayed different results for blood coagulation. TPP could enhance fibrin formation but take more time; while alginate-crosslinked chitosan might exhibit high water adsorption to intercept more blood and cells. However, surface-crosslinked chitosan might be neutralized on those positive ammonium groups, decreasing the positive charges on surfaces and also the adsorption and activation of platelets.

幾丁聚醣奈米線之製備及其在止血材料之應用 = The preparation of chitosan nanowires and its application to hemostasis
何, 應強

幾丁聚醣奈米線之製備及其在止血材料之應用 = The preparation of chitosan nanowires and its application to hemostasis / 何應強撰 - [高雄市] : 撰者, 2013[民102]. - 83面 ; 圖，表格 ; 30公分.
參考書目：面69-72102年10月31日公開.
奈米線nanowires

幾丁聚醣奈米線之製備及其在止血材料之應用 = The preparation of chitosan nanowires and its application to hemostasis
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