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降低二次代謝物干擾對蝴蝶蘭母瓶芽體增生效率之研究 = Studies o...

國立高雄大學生物科技研究所

降低二次代謝物干擾對蝴蝶蘭母瓶芽體增生效率之研究 = Studies on the Proliferation Efficiency of Phalaenopsis Buds at Mother Flask Stage by Reducing Interference of Secondary Metabolites

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Studies on the Proliferation Efficiency of Phalaenopsis Buds at Mother Flask Stage by Reducing Interference of Secondary Metabolites
作者:	陳煜升,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	2013[民102]
面頁冊數:	112面圖，表 : 30公分;
標題:	蝴蝶蘭
標題:	Phenolic compounds
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/23718004888736757907
附註:	105年10月25日公開
附註:	參考書目：面81-91
摘要註:	誘導花梗芽增生繁殖種苗是國內蝴蝶蘭業者大量增生分生苗的主要方式之一。雖然此增殖技術已廣泛地被採用，但是培植體釋放的酚類物質，導致培養基褐化會抑制培植體生長及新芽增生仍困擾業者。Polyvinylpyrrolidone（PVP）具吸附酚類化合物的特性。本論文調查不同蝴蝶蘭品系在母瓶階段芽體增生的最適PVP濃度，並透過生理與生化分析，探討PVP處理可以促進芽體增生的可能機制。結果顯示，適當的PVP濃度可以顯著促進芽體增生與培植體生長，尤其是一些具有市場價值但不易增殖品系，而且不同品系的最適PVP濃度並不相同。PVP處理不會顯著改變培植體內的H2O2濃度與catalase、ascorbate peroxidase、peroxidase與polyphenol oxidase活性表現；然而會降低培養基褐化程度與增加培養基的總酚類濃度，同時增加培植體內醣類消耗與cytosolic malate dehydrogenase、phenylcoumaran benzylic ether reductase、glyceraldehyde 3- phosphate dehydrogenase表現量。上述蛋白質分別參與胺基酸生成、維管束細胞分化與醣類降解。因此，PVP促進芽體增生的機制可能是與抗氧化相關表現無關，而係藉由降低酚類代謝物的干擾，並增加培植體內的醣類利用與相關蛋白質表現。此外，PVP處理會增加瓶內乙烯濃度，因此乙烯是直接或是其它荷爾蒙間接透過乙烯參與蝴蝶蘭芽體增生則仍然有待進一步釐清。 Flower stalk bud induction is one of the major methods for mass propagation of Phalaenopsis plantlets in vitro adopted by domestic growers. Although this propagative technique has been widely adopted, growers still face challenges related to phenolic exudation from explants, which results in medium browning that limits the growth of explants and the proliferation of buds. Polyvinylpyrrolidone（PVP）can absorb phenolic compounds. In this thesis, the optimal PVP concentration for promoting efficient bud propagation for numerous Phalaenopsis cultivars at the mother flask stage is investigated and the potential propagation mechanism is studied by conducting physiological and biochemical analyses. The results showed that the optimal PVP concentration can efficiently promote bud propagation and explant growth, particularly in several Phalaenopsis cultivars that have market value but not easy to propagate; furthermore, the optimum concentration depended on the various cultivars. Although PVP application did not significantly affect the concentration of H2O2 and the activity of catalase, ascorbate peroxidase, peroxidase, and polyphenol oxidase in the explants. However, it reduced the extent of browning, increased the total phenolics concentration in the medium, enhanced the consumption of soluble sugars, and promoted the expression of cytosolic malate dehydrogenase, phenylcoumaran benzylic ether reductase, and glyceraldehyde 3- phosphate dehydrogenase in the explants. These proteins involve in amino acid anabolism, vascular cell differentiation, and carbohydrate catabolism, respectively. Therefore, the mechanism for promoting bud proliferation using PVP is possibly not dependent on antioxidative-related expression, but instead closely related to reducing the interference of phenolic oxides in the medium and increasing the carbohydrate utilization and related protein expression in the explant. Furthermore, PVP treatment can increase the concentration of ethylene in the flask. However, whether ethylene（directly）or other hormones（indirectly）contribute to the bud proliferation of Phalaenopsis requires further clarification.

降低二次代謝物干擾對蝴蝶蘭母瓶芽體增生效率之研究 = Studies on the Proliferation Efficiency of Phalaenopsis Buds at Mother Flask Stage by Reducing Interference of Secondary Metabolites
陳, 煜升

降低二次代謝物干擾對蝴蝶蘭母瓶芽體增生效率之研究 = Studies on the Proliferation Efficiency of Phalaenopsis Buds at Mother Flask Stage by Reducing Interference of Secondary Metabolites / 陳煜升撰 - [高雄市] : 撰者, 2013[民102]. - 112面 ; 圖，表 ; 30公分.
105年10月25日公開參考書目：面81-91.
蝴蝶蘭Phenolic compounds

降低二次代謝物干擾對蝴蝶蘭母瓶芽體增生效率之研究 = Studies on the Proliferation Efficiency of Phalaenopsis Buds at Mother Flask Stage by Reducing Interference of Secondary Metabolites
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330 $a 誘導花梗芽增生繁殖種苗是國內蝴蝶蘭業者大量增生分生苗的主要方式之一。雖然此增殖技術已廣泛地被採用，但是培植體釋放的酚類物質，導致培養基褐化會抑制培植體生長及新芽增生仍困擾業者。Polyvinylpyrrolidone（PVP）具吸附酚類化合物的特性。本論文調查不同蝴蝶蘭品系在母瓶階段芽體增生的最適PVP濃度，並透過生理與生化分析，探討PVP處理可以促進芽體增生的可能機制。結果顯示，適當的PVP濃度可以顯著促進芽體增生與培植體生長，尤其是一些具有市場價值但不易增殖品系，而且不同品系的最適PVP濃度並不相同。PVP處理不會顯著改變培植體內的H2O2濃度與catalase、ascorbate peroxidase、peroxidase與polyphenol oxidase活性表現；然而會降低培養基褐化程度與增加培養基的總酚類濃度，同時增加培植體內醣類消耗與cytosolic malate dehydrogenase、phenylcoumaran benzylic ether reductase、glyceraldehyde 3- phosphate dehydrogenase表現量。上述蛋白質分別參與胺基酸生成、維管束細胞分化與醣類降解。因此，PVP促進芽體增生的機制可能是與抗氧化相關表現無關，而係藉由降低酚類代謝物的干擾，並增加培植體內的醣類利用與相關蛋白質表現。此外，PVP處理會增加瓶內乙烯濃度，因此乙烯是直接或是其它荷爾蒙間接透過乙烯參與蝴蝶蘭芽體增生則仍然有待進一步釐清。 Flower stalk bud induction is one of the major methods for mass propagation of Phalaenopsis plantlets in vitro adopted by domestic growers. Although this propagative technique has been widely adopted, growers still face challenges related to phenolic exudation from explants, which results in medium browning that limits the growth of explants and the proliferation of buds. Polyvinylpyrrolidone（PVP）can absorb phenolic compounds. In this thesis, the optimal PVP concentration for promoting efficient bud propagation for numerous Phalaenopsis cultivars at the mother flask stage is investigated and the potential propagation mechanism is studied by conducting physiological and biochemical analyses. The results showed that the optimal PVP concentration can efficiently promote bud propagation and explant growth, particularly in several Phalaenopsis cultivars that have market value but not easy to propagate; furthermore, the optimum concentration depended on the various cultivars. Although PVP application did not significantly affect the concentration of H2O2 and the activity of catalase, ascorbate peroxidase, peroxidase, and polyphenol oxidase in the explants. However, it reduced the extent of browning, increased the total phenolics concentration in the medium, enhanced the consumption of soluble sugars, and promoted the expression of cytosolic malate dehydrogenase, phenylcoumaran benzylic ether reductase, and glyceraldehyde 3- phosphate dehydrogenase in the explants. These proteins involve in amino acid anabolism, vascular cell differentiation, and carbohydrate catabolism, respectively. Therefore, the mechanism for promoting bud proliferation using PVP is possibly not dependent on antioxidative-related expression, but instead closely related to reducing the interference of phenolic oxides in the medium and increasing the carbohydrate utilization and related protein expression in the explant. Furthermore, PVP treatment can increase the concentration of ethylene in the flask. However, whether ethylene（directly）or other hormones（indirectly）contribute to the bud proliferation of Phalaenopsis requires further clarification.
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