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紫花丹參多倍體生長與藥用成份分析 = Growth and medici...

國立高雄大學生命科學系碩士班

紫花丹參多倍體生長與藥用成份分析 = Growth and medicinal constituents in polyploids of Salvia miltiorhiza bge

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Growth and medicinal constituents in polyploids of Salvia miltiorhiza bge
作者:	甘芳儒,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	2014[民103]
面頁冊數:	48面彩圖，表 : 30公分;
標題:	紫花丹參
標題:	Salvia miltiorhiza
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/14455729782792400426
附註:	參考書目：面38-41
附註:	103年12月16日公開
摘要註:	紫花丹參（Salvia miltiorhiza bge.）為唇形科多年生草本植物，開紫花，葉似薄荷，紫花丹參的藥用部位為其紅色的乾燥根，為高經濟價值的中藥材。本論文培育紫花丹參的多倍體，並比較二倍體及四倍體的生長形態及藥用成份之差異。多倍體主要是指染色體大於正常染色體數目之植物，其特徵為具有較大的營養器官，較高的二次代謝物含量，以及對逆境有較高的抗性。因此，培育發展藥用植物的多倍體具有商業價值。本論文所培育之兩品系四倍體，係經由1 mg/l或2 mg/l秋水仙素誘導葉培植體並經由流式細胞技術鑑定而得，分別命名為4x-1及4x-2。將二倍體（簡稱2x）、4x-1及4x-2的莖段培育於改良式1/2MS培養基，培養容器為蘭花瓶。試驗結果顯示， 75天後，莖段抽長並發根成為小瓶苗，其中4x-1及4x-2小植株之鮮重（分別為10.31 g及9.96 g）均比2x高；4x-1及4x-2小植株莖段高度皆顯著較高（分別為2.63 cm及3.15 cm），葉片數亦最多（18.4及18.5）。雖然2x根部最長（8.7 cm），但都比較細（0.8 mm）；4x-1長度次之（6.23 cm），根部顯著較粗（1.05 mm）；4x-2根部最短（4.46 cm），大多都是粗狀根（1.09 mm）。4x-1及4x-2小植株氣孔的長和寬（分別為17.1及19.9 μm；17.8及19.1 μm）均顯著高於2x （11.5和14.7 μm），而氣孔頻率則是2x （29.0）較高。植株成熟後，4x的葉片較圓，其葉面積亦增大。4x花粉粒的長及寬（38.9及30.0 μm）顯著大於2x （30.8及21.7 μm）。4x種子比2x較大且較圓（0.487 mm; 0.378 mm）。藥用成分方面，使用栽培6個月的丹參植株之組織做為樣本進行分析，其結果顯示，四倍體植株根部之丹參酮IIA及隱丹參酮具有比二倍體較高的含量。 Chinese sage, Salvia miltiorhiza bge., is a perennial plant which belongs to Labiatae Family. It has purple flowers and the leaves look like Peppermint’s leaves.The red dried root has been used as Chinese medicine to cure several diseases. Chinese sage is a traditional Chinese medicine with high economical value. This study compareed Diploid plants to two lines of tetraploid plants in their morphology growth, development and also medicinal constituents. Polyploid plants contain more sets of chromosomes than diploid ones. Polypoid Plants can grow stronger, have more stress tolerance, and increasing levels of secondary metabolites. As a result, the development of induced polyploid medicinal herbs might have an impact on its commercial value. To obtain the tetraploid plants, leaves were treated with 1 mg/l and 2 mg/l colchicine, named 4x-1 and 4x-2. Ploidy levels of donor plants were proved using flow cytometry. Diploid (2x), 4x-1 and 4x-2 were cultured on half strength Murashige and Skoog (MS) medium. After 75 days of culture, the in vitro shoots formed roots and converted into plantlets. The results showed that tetraploid 4x-1 and 4x-2 plants have significantly higher fresh weight of 10.31 g and 9.96 g when compared to 2x plants (5.29 g). Tetraploid plants gave higher plant height (2.63 cm and 3.15 cm) ,more leaves (18.4 and 18.5) than 2x plants. Although diploid had longer roots (8.7 cm), but their roots have smaller diemeters (0.8 mm). The length of 4x-1 roots was 6.23 cm and the meandiemeter was 1.05 mm. 4x-2 roots were shorter (4.46 cm) and thicker (1.09 mm). Tetraploid had larger stomata with higher length and width (17.1 and 19.9 μm; 17.8 and 19.1 μm). However, diploid had higher frequency of stomata (29.0) than 4x plants. Tetraploid leaves changed into a circle like shape, increasing the total leaf area. In addition, pollen of tetraploid have higher length and width (38.9 and 30.0 μm) than 2x plants (30.8 and 21.7 μm). Seed of tetraploid plants were also larger (0.487 mm; 0.378 mm) than 2x plants. Root tissues from six-month-old plants were used to analysed the medicinal constituents using HPLC. It was found that the 4x plants have more tanshinone IIA and salcianolic acids B than 2x plants.

紫花丹參多倍體生長與藥用成份分析 = Growth and medicinal constituents in polyploids of Salvia miltiorhiza bge
甘, 芳儒

紫花丹參多倍體生長與藥用成份分析 = Growth and medicinal constituents in polyploids of Salvia miltiorhiza bge / 甘芳儒撰 - [高雄市] : 撰者, 2014[民103]. - 48面 ; 彩圖，表 ; 30公分.
參考書目：面38-41103年12月16日公開.
紫花丹參Salvia miltiorhiza

紫花丹參多倍體生長與藥用成份分析 = Growth and medicinal constituents in polyploids of Salvia miltiorhiza bge
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