高雄市登革熱病媒蚊對殺蟲劑之感受性研究 = Insecticide su...
國立高雄大學運動健康與休閒學系碩士班

 

  • 高雄市登革熱病媒蚊對殺蟲劑之感受性研究 = Insecticide susceptibility of dengue fever vectors in Kaohsiung city
  • 紀錄類型: 書目-語言資料,印刷品 : 單行本
    並列題名: Insecticide susceptibility of dengue fever vectors in Kaohsiung city
    作者: 詹雅筑,
    其他團體作者: 國立高雄大學
    出版地: [高雄市]
    出版者: 撰者;
    出版年: 2014[民103]
    面頁冊數: 115, [6]面圖,表 : 30公分;
    標題: 埃及斑蚊
    標題: Aedes aegypti
    電子資源: http://handle.ncl.edu.tw/11296/ndltd/17366485419020344598
    附註: 參考書目:面50-55
    附註: 103年12月16日公開
    摘要註: 登革熱為台灣重要的蟲媒傳播疾病,主要病媒蚊為埃及斑蚊(Aedes aegypti)及白線斑蚊(Aedes albopictus)。過去研究發現,高雄地區埃及斑蚊對依芬寧、百滅寧已產生抗藥性,因此瞭解高雄地區埃及斑蚊及白線斑蚊對殺蟲藥劑抗藥性為本研究之重要目標。 本研究於2013年4-5月,以誘蚊產卵器收集高雄地區(三民區、前鎮區、苓雅區、小港區、左營區、彌陀區)埃及斑蚊及白線斑蚊蟲卵,並帶回實驗室飼養繁殖。實驗使用WHO藥膜測定法、幼蟲浸漬法、成蚊局部微量滴定法、成蚊藥膜測定法等試驗,測試高雄不同行政區埃及斑蚊及白線斑蚊對於殺蟲劑的感受性,以瞭解各品系對不同殺蟲劑的抗藥性程度。 世界衛生組織(WHO)藥膜測定法試驗結果顯示,高雄地區埃及斑蚊對合成除蟲菊酯類殺蟲劑皆已產生抗藥性,氨基甲酸鹽類殺蟲劑則可能產生抗藥性,僅有機磷類殺蟲劑皆未產生抗藥性。白線斑蚊對氨基甲酸鹽類殺蟲劑則可能產生抗藥性,合成除蟲菊酯類及有機磷類殺蟲劑皆未產生抗藥性。幼蟲浸漬試驗結果顯示,高雄6個行政區埃及斑蚊幼蟲對百滅寧抗性比為高度抗藥性;白線斑蚊幼蟲對百滅寧抗性比皆尚未產生抗藥性。成蚊局部微量滴定試驗結果顯示,高雄6個行政區埃及斑蚊成蚊對百滅寧抗性比為高度抗藥性;白線斑蚊除三民區外,其他品系白線斑蚊成蚊對百滅寧抗性比尚未產生抗藥性。百滅寧加piperonyl butoxide (PBO)協力劑試驗結果顯示,發現不同比例百滅寧加PBO協力劑以1:10比例效果最佳,且PBO協力劑對白線斑蚊作用較埃及斑蚊明顯。成蚊自製藥膜測定試驗結果顯示,高雄6個行政區埃及斑蚊成蚊對百滅寧抗性比為中度抗藥性,白線斑蚊成蚊對百滅寧抗性比皆尚未產生抗藥性。 綜合實驗結果,高雄地區埃及斑蚊對合成除蟲菊酯類殺蟲劑產生嚴重抗藥性,部分區域白線斑蚊則對氨基甲酸鹽類殺蟲劑已產生抗藥性。建議暫停施用已產生抗藥性的藥劑,且未來應審慎評估並規劃各地殺蟲劑的使用並規劃合理的藥劑使用策略,以免持續在野外篩選出抗藥性蚊蟲。 Dengue fever is a important infect born diseases in Taiwan. The major vectors are Aedes aegypti and Aedes albopictus. Some studies had found that Aedes aegypti had resistance to permethrin and etofenprox in Kaohsiung city, so it is important to understand the insecticide resistance for Aedes aegypti and Aedes albopictus in Kaohsiung city. This study was carried out during April to May in 2013. We used the ovitraps to collect the eggs of Aedes aegypti and Aedes albopictus in Kaohsiung city(Sanmin district, Lingya district, Qianzhen district, Xiaogang district, Zuoying district, Mituo district) and collected eggs were taken back to laboratory for future breed. The study were conducted by WHO membrane bioassay, larvae bioassay, topical application bioassay and membrane bioassay to test the insecticide resistance for Aedes aegypti and Aedes albopictus in Kaohsiung different districts. The result of WHO membrane bioassay show that Aedes aegypti in Kaohsiung city has resistance to pyrethroid and possibility of resistance to carbamate and susceptibility to organophosphate. Aedes albopictus has possibility of resistance to carbamate and has susceptibility to pyrethroid and organophosphate.The result of larvae bioassay show that Aedes aegypti in Kaohsiung city has high resistance to permethrin and Aedes albopictus has susceptibility to permethrin. The result of topical application bioassay show that Aedes aegypti in Kaohsiung city has high resistance to permethrin and Aedes albopictus has susceptibility to permethrin except Sanmin district. The result of adding the PBO to permethrin show that 1:10 has the best influence and effect Aedes albopictus better than Aedes aegypti. The result of membrane bioassay Aedes aegypti in Kaohsiung city has medium resistance to permethrin and Aedes albopictus has susceptibility to permethrin.The study hope to understand the insecticide resistance for dengue fever vectors in different districts and establish a regional insecticide index to provide a reference of prevention and control of dengue fever.
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310002492869 博碩士論文區(二樓) 不外借資料 學位論文 TH 008M/0019 896219 2778 2014 一般使用(Normal) 在架 0
310002492877 博碩士論文區(二樓) 不外借資料 學位論文 TH 008M/0019 896219 2778 2014 c.2 一般使用(Normal) 在架 0
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