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Using mass spectrometry for biochemi...

Jenner, Matthew.

Using mass spectrometry for biochemical studies on enzymatic domains from polyketide synthases

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Using mass spectrometry for biochemical studies on enzymatic domains from polyketide synthasesby Matthew Jenner.
作者:	Jenner, Matthew.
出版者:	Cham :Springer International Publishing :2016.
面頁冊數:	xviii, 176 p. :ill., digital ;24 cm.
Contained By:	Springer eBooks
標題:	PolyketidesSpectra.
電子資源:	http://dx.doi.org/10.1007/978-3-319-32723-5
ISBN:	9783319327235$q(electronic bk.)

Using mass spectrometry for biochemical studies on enzymatic domains from polyketide synthases
Jenner, Matthew.

Using mass spectrometry for biochemical studies on enzymatic domains from polyketide synthases[electronic resource] /by Matthew Jenner. - Cham :Springer International Publishing :2016. - xviii, 176 p. :ill., digital ;24 cm. - Springer theses,2190-5053. - Springer theses..

Introduction -- Materials and Methods -- Substrate Specificity of Ketosynthase Domains Part I: β-Branched Acyl Chains -- Substrate Specificity of Ketosynthase Domains Part II: Amino Acid-Containing Acyl Chains -- Synthesis of Acyl-Acyl Carrier Proteins and their use in Studying Polyketide Synthase Enzymology -- Substrate Specificity of Ketosynthase Domains Part III: Elongation-Based Substrate Specificity.

This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs) Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, Matthew Jenner has successfully studied the specificity of a range of KS domains from the bacillaene and psymberin PKSs with regard to the initial acylation step of KS-catalysis. The findings in this thesis provide important insights into mechanisms of KS specificity and show that mutagenesis can be used to expand the repertoire of acceptable substrates for future PKS engineering. The documentation of this research is a useful reference and guideline for students starting a PhD in this field.

ISBN: 9783319327235$q(electronic bk.)

Standard No.: 10.1007/978-3-319-32723-5doiSubjects--Topical Terms:

744430
Polyketides
--Spectra.

LC Class. No.: QP752.P65

Dewey Class. No.: 572.45

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