國立高雄大學圖資館 |

語系: 繁體中文

說明(常見問題)

圖資館首頁

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Growth-coupled Metabolic Engineering...

Mehrer, Christopher R.

Growth-coupled Metabolic Engineering for High-yield Chemical Production.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Growth-coupled Metabolic Engineering for High-yield Chemical Production.
作者:	Mehrer, Christopher R.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, 2019
面頁冊數:	184 p.
附註:	Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
附註:	Advisor: Pfleger, Brian F.
Contained By:	Dissertations Abstracts International81-04B.
標題:	Chemical engineering.
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13808170
ISBN:	9781687972880

Growth-coupled Metabolic Engineering for High-yield Chemical Production.
Mehrer, Christopher R.

Growth-coupled Metabolic Engineering for High-yield Chemical Production. - Ann Arbor : ProQuest Dissertations & Theses, 2019 - 184 p.

Source: Dissertations Abstracts International, Volume: 81-04, Section: B.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2019.

This item must not be sold to any third party vendors.

Oleochemicals are a class of organic chemicals found in fuels, materials, and consumer products. While oleochemicals are historically derived from petroleum and plant oils, microbial bioprocesses are seen as a major alternative to traditional chemical production. Improvements in bioprocess performance can be performed at multiple scales: molecular (gene expression), pathway, cell, small-scale fermentation, and fermentation scale-up. Here, two examples of oleochemical bioprocess development are examined. In the first example, a process to produce medium chain-length n-alcohols from glucose in Escherichia coli is developed. In this example, strain design was performed using a genome-scale metabolic model to build a growth-coupled strain to improve product yields. Techniques of synthetic biology were then employed to evaluate pathway variants and build an anaerobically autoinduced strain capable of producing 1.8 g/L of n-alcohols.In the second example, a bioconversion process to perform complete bioconversion of levulinic acid, a common catalytic product from biomass, to 2-butanone (methyl ethyl ketone) is developed. As with the first example, a genome-scale metabolic model was employed for growth-coupled strain design allowing for complete conversion. Following initial demonstration of the bioconversion, the process was integrated with furfuryl alcohol-derived levulinic acid, simulating a biomass-derived levulinic acid that would be employed in industrial production.Following the two, more full examples of bioprocess development, two other aspects of bioprocess development are studied. First, the beginnings of bioprocess scale-up are examined by scaling an octanoic acid-producing strain of E. coli to a high cell density fed-batch fermentation. A kinetic model is employed to analyze the fermentation and determine that octanoic acid toxicity is a major factor limiting process performance.Next, the focus is directed toward the beginning of bioprocess development from a strain-selection perspective. From previous knowledge concerning the phenotypic differences between E. coli strains MG1655 and LS5218, the genotypic differences between the two strains are examined. Finally, future directions of the field discussed. Particular focus is placed upon building useful kinetic models for strain design, scale-down and scale-up experiments, and potential new products for microbial process development.

ISBN: 9781687972880Subjects--Topical Terms:

206267
Chemical engineering.

Growth-coupled Metabolic Engineering for High-yield Chemical Production.
LDR:03439nmm a2200313 4500 001 570715
005 20200514111944.5
008 200901s2019 ||||||||||||||||| ||eng d
020 $a 9781687972880
035 $a (MiAaPQ)AAI13808170
035 $a AAI13808170
040 $a MiAaPQ $c MiAaPQ
100 1 $a Mehrer, Christopher R. $3 857366
245 1 0 $a Growth-coupled Metabolic Engineering for High-yield Chemical Production.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2019
300 $a 184 p.
500 $a Source: Dissertations Abstracts International, Volume: 81-04, Section: B.
500 $a Advisor: Pfleger, Brian F.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2019.
506 $a This item must not be sold to any third party vendors.
520 $a Oleochemicals are a class of organic chemicals found in fuels, materials, and consumer products. While oleochemicals are historically derived from petroleum and plant oils, microbial bioprocesses are seen as a major alternative to traditional chemical production. Improvements in bioprocess performance can be performed at multiple scales: molecular (gene expression), pathway, cell, small-scale fermentation, and fermentation scale-up. Here, two examples of oleochemical bioprocess development are examined. In the first example, a process to produce medium chain-length n-alcohols from glucose in Escherichia coli is developed. In this example, strain design was performed using a genome-scale metabolic model to build a growth-coupled strain to improve product yields. Techniques of synthetic biology were then employed to evaluate pathway variants and build an anaerobically autoinduced strain capable of producing 1.8 g/L of n-alcohols.In the second example, a bioconversion process to perform complete bioconversion of levulinic acid, a common catalytic product from biomass, to 2-butanone (methyl ethyl ketone) is developed. As with the first example, a genome-scale metabolic model was employed for growth-coupled strain design allowing for complete conversion. Following initial demonstration of the bioconversion, the process was integrated with furfuryl alcohol-derived levulinic acid, simulating a biomass-derived levulinic acid that would be employed in industrial production.Following the two, more full examples of bioprocess development, two other aspects of bioprocess development are studied. First, the beginnings of bioprocess scale-up are examined by scaling an octanoic acid-producing strain of E. coli to a high cell density fed-batch fermentation. A kinetic model is employed to analyze the fermentation and determine that octanoic acid toxicity is a major factor limiting process performance.Next, the focus is directed toward the beginning of bioprocess development from a strain-selection perspective. From previous knowledge concerning the phenotypic differences between E. coli strains MG1655 and LS5218, the genotypic differences between the two strains are examined. Finally, future directions of the field discussed. Particular focus is placed upon building useful kinetic models for strain design, scale-down and scale-up experiments, and potential new products for microbial process development.
590 $a School code: 0262.
650 4 $a Chemical engineering. $3 206267
650 4 $a Biochemistry. $3 188609
650 4 $a Microbiology. $3 192943
690 $a 0542
690 $a 0487
690 $a 0410
710 2 $a The University of Wisconsin - Madison. $b Chemical Engineering. $3 603243
773 0 $t Dissertations Abstracts International $g 81-04B.
790 $a 0262
791 $a Ph.D.
792 $a 2019
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=13808170