國立高雄大學圖資館 |

語系: 繁體中文

說明(常見問題)

圖資館首頁

登入

回首頁 到查詢結果 [ subject:"Biological assay." ]

切換: 標籤 | MARC模式 | ISBD

One-step generation of a drug-releas...

Song, Seo Woo.

One-step generation of a drug-releasing microarray for high-throughput small-volume bioassays

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	One-step generation of a drug-releasing microarray for high-throughput small-volume bioassaysby Seo Woo Song.
作者:	Song, Seo Woo.
出版者:	Singapore :Springer Singapore :2019.
面頁冊數:	xxi, 52 p. :ill., digital ;24 cm.
Contained By:	Springer eBooks
標題:	DrugsTesting.
電子資源:	https://doi.org/10.1007/978-981-13-8094-5
ISBN:	9789811380945$q(electronic bk.)

One-step generation of a drug-releasing microarray for high-throughput small-volume bioassays
Song, Seo Woo.

One-step generation of a drug-releasing microarray for high-throughput small-volume bioassays[electronic resource] /by Seo Woo Song. - Singapore :Springer Singapore :2019. - xxi, 52 p. :ill., digital ;24 cm. - Springer theses,2190-5053. - Springer theses..

Abstract -- Table of Contents -- List of Tables -- List of Figures -- Chapter 1. Introduction -- 1.1. High-Throughput Small-Volume Bioassays -- 1.2. Developmental Goal for the 'Pipetting-Free' HTS Platforms -- 1.3. Main Concept: One-Step Generation of a Drug-Releasing Microarray-on-a-Chip by Self-Assembly of Drug-Laden Microparticles (DLPs) -- Chapter 2. System Development -- 2.1. Sealing-Film Assisted Seeding Method for Saving Cell Consumptions -- 2.2. Chip and Jig Development -- 2.3. Preparation of DLPs Library -- 2.4. Decoding Microparticles -- 2.5. Statistical Analysis for Duplications -- Chapter 3. Application: Screening of Sequential Drug Combinations -- 3.1. Therapeutic Benefit of Sequential Drug Combination Based on Rewiring of Intracellular Pathways -- 3.2. Screening of Sequential Drug Combination Using a Partipetting Platform -- 3.3. Proof-of-Concept: Sequential Combinatorial Cell Staining Assay by Replacement of the Drug Chip -- 3.4. Screening of Sequential Combinatorial Drugs with EGFR Inhibitor Followed by Genotoxin against Triple Negative Breast Cancer (TNBC) -- Chapter 4. Conclusion and Discussion -- Bibliography.

This thesis demonstrates a technology that enables pipetting-free high-throughput screening (HTS) on a miniaturized platform, eliminating the need for thousands of one-by-one pipetting and conventional liquid handling systems. This platform enhances accessibility to HTS and enables HTS to be used in small-to-medium scale laboratories. In addition, it allows large-scale combinatorial screening with a small number of valuable cells, such as patients' primary cancer cells. This technique will have a high impact for widespread use of HTS in the era of personalized medicine. In this thesis, the author firstly describes the need and concept of 'partipetting' for pipetting-free HTS platform. It is realized by the one-step pipetting and self-assembly of encoded drug-laden microparticles (DLPs) on the microwells. Next, the technical implementations required for the platform demonstration are described. It includes preparation of encoded DLPs, plastic chip fabrication, and realization of automated system. Lastly, screening of sequential drug combinations using this platform is demonstrated. This shows the potential of the proposed technology for various applications.

ISBN: 9789811380945$q(electronic bk.)

Standard No.: 10.1007/978-981-13-8094-5doiSubjects--Topical Terms:

183737
Drugs
--Testing.

LC Class. No.: RM301.27 / .S664 2019

Dewey Class. No.: 615.704

One-step generation of a drug-releasing microarray for high-throughput small-volume bioassays
LDR:03366nmm a2200337 a 4500 001 558878
003 DE-He213
005 20191025161846.0
006 m d
007 cr nn 008maaau
008 191219s2019 si s 0 eng d
020 $a 9789811380945$q(electronic bk.)
020 $a 9789811380938$q(paper)
024 7 $a 10.1007/978-981-13-8094-5 $2 doi
035 $a 978-981-13-8094-5
040 $a GP $c GP
041 0 $a eng
050 4 $a RM301.27 $b .S664 2019
072 7 $a MQW $2 bicssc
072 7 $a TEC009000 $2 bisacsh
072 7 $a MQW $2 thema
082 0 4 $a 615.704 $2 23
090 $a RM301.27 $b .S698 2019
100 1 $a Song, Seo Woo. $3 841679
245 1 0 $a One-step generation of a drug-releasing microarray for high-throughput small-volume bioassays $h [electronic resource] / $c by Seo Woo Song.
260 $a Singapore : $b Springer Singapore : $b Imprint: Springer, $c 2019.
300 $a xxi, 52 p. : $b ill., digital ; $c 24 cm.
490 1 $a Springer theses, $x 2190-5053
505 0 $a Abstract -- Table of Contents -- List of Tables -- List of Figures -- Chapter 1. Introduction -- 1.1. High-Throughput Small-Volume Bioassays -- 1.2. Developmental Goal for the 'Pipetting-Free' HTS Platforms -- 1.3. Main Concept: One-Step Generation of a Drug-Releasing Microarray-on-a-Chip by Self-Assembly of Drug-Laden Microparticles (DLPs) -- Chapter 2. System Development -- 2.1. Sealing-Film Assisted Seeding Method for Saving Cell Consumptions -- 2.2. Chip and Jig Development -- 2.3. Preparation of DLPs Library -- 2.4. Decoding Microparticles -- 2.5. Statistical Analysis for Duplications -- Chapter 3. Application: Screening of Sequential Drug Combinations -- 3.1. Therapeutic Benefit of Sequential Drug Combination Based on Rewiring of Intracellular Pathways -- 3.2. Screening of Sequential Drug Combination Using a Partipetting Platform -- 3.3. Proof-of-Concept: Sequential Combinatorial Cell Staining Assay by Replacement of the Drug Chip -- 3.4. Screening of Sequential Combinatorial Drugs with EGFR Inhibitor Followed by Genotoxin against Triple Negative Breast Cancer (TNBC) -- Chapter 4. Conclusion and Discussion -- Bibliography.
520 $a This thesis demonstrates a technology that enables pipetting-free high-throughput screening (HTS) on a miniaturized platform, eliminating the need for thousands of one-by-one pipetting and conventional liquid handling systems. This platform enhances accessibility to HTS and enables HTS to be used in small-to-medium scale laboratories. In addition, it allows large-scale combinatorial screening with a small number of valuable cells, such as patients' primary cancer cells. This technique will have a high impact for widespread use of HTS in the era of personalized medicine. In this thesis, the author firstly describes the need and concept of 'partipetting' for pipetting-free HTS platform. It is realized by the one-step pipetting and self-assembly of encoded drug-laden microparticles (DLPs) on the microwells. Next, the technical implementations required for the platform demonstration are described. It includes preparation of encoded DLPs, plastic chip fabrication, and realization of automated system. Lastly, screening of sequential drug combinations using this platform is demonstrated. This shows the potential of the proposed technology for various applications.
650 0 $a Drugs $x Testing. $3 183737
650 0 $a Biological assay. $3 682866
650 1 4 $a Biomedical Engineering and Bioengineering. $3 826326
650 2 4 $a Microarrays. $3 342827
650 2 4 $a Soft and Granular Matter, Complex Fluids and Microfluidics. $3 379754
650 2 4 $a Nanotechnology and Microengineering. $3 348421
650 2 4 $a Applied Microbiology. $3 273946
650 2 4 $a Microengineering. $3 484493
710 2 $a SpringerLink (Online service) $3 273601
773 0 $t Springer eBooks
830 0 $a Springer theses. $3 557607
856 4 0 $u https://doi.org/10.1007/978-981-13-8094-5
950 $a Engineering (Springer-11647)