國立高雄大學圖資館 |

語系: 繁體中文

說明(常見問題)

圖資館首頁

登入

回首頁 到查詢結果 [ subject:"Molecular cloning." ]

切換: 標籤 | MARC模式 | ISBD

DNA cloninga hands-on approach /

Choi, Seok-Yong.

DNA cloninga hands-on approach /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	DNA cloningby Seok-Yong Choi, Hyunju Ro, Hankuil Yi.
其他題名:	a hands-on approach /
作者:	Choi, Seok-Yong.
其他作者:	Ro, Hyunju.
出版者:	Dordrecht :Springer Netherlands :2019.
面頁冊數:	xiii, 131 p. :ill., digital ;24 cm.
Contained By:	Springer eBooks
標題:	Molecular cloning.
電子資源:	https://doi.org/10.1007/978-94-024-1662-6
ISBN:	9789402416626$q(electronic bk.)

DNA cloninga hands-on approach /
Choi, Seok-Yong.

DNA cloninga hands-on approach /[electronic resource] :by Seok-Yong Choi, Hyunju Ro, Hankuil Yi. - Dordrecht :Springer Netherlands :2019. - xiii, 131 p. :ill., digital ;24 cm.

Part I. What is cloning? -- Chapter 1.1 Definition of cloning -- Chapter 1.2 Discovering a new gene -- Chapter 1.3 Cloning in the past -- Chapter 1.4 Cloning in the present -- Part II. A prerequisite for cloning -- Chapter 2.1 Software useful for cloning design -- Chapter 2.2 Vector, plasmid, construct and the Kozak consensus sequence -- Chapter 2.3 Multiple cloning sites (MCS) -- Chapter 2.4 Restriction endonucleases and star activity -- Chapter 2.5 Agarose gel electrophoresis -- Chapter 2.6 Pouring LB agar plates -- Chapter 2.7 Competent cells -- Chapter 2.8 The conversion of DNA mass into molar concentration -- Chapter 2.9 Upon receiving new plasmids -- Chapter 2.10 cDNA library -- Part III. The first step in cloning -- Chapter 3.1 Cut & paste -- Chapter 3.2 DNA sequencing and direct sequencing -- Chapter 3.3 PCR and nested PCR -- Chapter 3.4 Fill-in (Full & Partial) -- Chapter 3.5 Compatible cohesive ends -- Chapter 3.6 Methylation -- Chapter 3.7 Three-piece ligation -- Chapter 3.8 Site-directed mutagenesis -- Chapter 3.9 Structure of plant transformation vectors -- Chapter 3.10 Transformation of R. radiobacter -- Part IV. The next step of cloning -- Chapter 4.1 How to insert a DNA fragment into a gene -- Chapter 4.2 How to delete an internal region of a gene -- Chapter 4.3 How to insert an epitope tag into a gene -- Chapter 4.4 Translational fusion vs. transcriptional fusion -- Part V. The last steps of cloning -- Chapter 5.1 Method for cloning similar genes in different species -- Chapter 5.2 RACE (rapid amplification of cDNA ends) -- Chapter 5.3 BAC recombineering -- Chapter 5.4 Old trick: partial digestion -- Chapter 5.5 Modification of a vector -- Chapter 5.6 When you notice a frame shift mutation upon cloning -- Chapter 5.7 Reality of cloning: an extremely unlucky case -- Part VI. Methods that make your cloning life easier -- Chapter 6.1 TA cloning and production of a T-vector -- Chapter 6.2 TOPO TA cloning -- Chapter 6.3 Gateway cloning -- Chapter 6.4 Golden Gate Assembly for a modular cloning -- Chapter 6.5 In-Fusion Sequence and Ligation-Independent Cloning (In-Fusion SLIC) -- Chapter 6.6 T4 DNA Polymerase Sequence-and Ligation-Independent Cloning (T4 DNA Pol SLIC) -- Chapter 6.7 Non-template PCR cloning -- Part VII. Advice to cloners -- Chapter 7.1 When cloning is not going well -- Chapter 7.2 Keep your cloning data organized -- Appendix 1. Further readings -- Appendix 2. Abbreviations -- Appendix 3. Index.

This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.

ISBN: 9789402416626$q(electronic bk.)

Standard No.: 10.1007/978-94-024-1662-6doiSubjects--Topical Terms:

197743
Molecular cloning.

LC Class. No.: QH442.2 / .C465 2019

Dewey Class. No.: 660.65

DNA cloninga hands-on approach /
LDR:04539nmm a2200325 a 4500 001 556630
003 DE-He213
005 20190917110701.0
006 m d
007 cr nn 008maaau
008 191127s2019 ne s 0 eng d
020 $a 9789402416626$q(electronic bk.)
020 $a 9789402416602$q(paper)
024 7 $a 10.1007/978-94-024-1662-6 $2 doi
035 $a 978-94-024-1662-6
040 $a GP $c GP
041 0 $a eng
050 4 $a QH442.2 $b .C465 2019
072 7 $a MFN $2 bicssc
072 7 $a MED107000 $2 bisacsh
072 7 $a MFN $2 thema
082 0 4 $a 660.65 $2 23
090 $a QH442.2 $b .C545 2019
100 1 $a Choi, Seok-Yong. $3 838857
245 1 0 $a DNA cloning $h [electronic resource] : $b a hands-on approach / $c by Seok-Yong Choi, Hyunju Ro, Hankuil Yi.
260 $a Dordrecht : $b Springer Netherlands : $b Imprint: Springer, $c 2019.
300 $a xiii, 131 p. : $b ill., digital ; $c 24 cm.
505 0 $a Part I. What is cloning? -- Chapter 1.1 Definition of cloning -- Chapter 1.2 Discovering a new gene -- Chapter 1.3 Cloning in the past -- Chapter 1.4 Cloning in the present -- Part II. A prerequisite for cloning -- Chapter 2.1 Software useful for cloning design -- Chapter 2.2 Vector, plasmid, construct and the Kozak consensus sequence -- Chapter 2.3 Multiple cloning sites (MCS) -- Chapter 2.4 Restriction endonucleases and star activity -- Chapter 2.5 Agarose gel electrophoresis -- Chapter 2.6 Pouring LB agar plates -- Chapter 2.7 Competent cells -- Chapter 2.8 The conversion of DNA mass into molar concentration -- Chapter 2.9 Upon receiving new plasmids -- Chapter 2.10 cDNA library -- Part III. The first step in cloning -- Chapter 3.1 Cut & paste -- Chapter 3.2 DNA sequencing and direct sequencing -- Chapter 3.3 PCR and nested PCR -- Chapter 3.4 Fill-in (Full & Partial) -- Chapter 3.5 Compatible cohesive ends -- Chapter 3.6 Methylation -- Chapter 3.7 Three-piece ligation -- Chapter 3.8 Site-directed mutagenesis -- Chapter 3.9 Structure of plant transformation vectors -- Chapter 3.10 Transformation of R. radiobacter -- Part IV. The next step of cloning -- Chapter 4.1 How to insert a DNA fragment into a gene -- Chapter 4.2 How to delete an internal region of a gene -- Chapter 4.3 How to insert an epitope tag into a gene -- Chapter 4.4 Translational fusion vs. transcriptional fusion -- Part V. The last steps of cloning -- Chapter 5.1 Method for cloning similar genes in different species -- Chapter 5.2 RACE (rapid amplification of cDNA ends) -- Chapter 5.3 BAC recombineering -- Chapter 5.4 Old trick: partial digestion -- Chapter 5.5 Modification of a vector -- Chapter 5.6 When you notice a frame shift mutation upon cloning -- Chapter 5.7 Reality of cloning: an extremely unlucky case -- Part VI. Methods that make your cloning life easier -- Chapter 6.1 TA cloning and production of a T-vector -- Chapter 6.2 TOPO TA cloning -- Chapter 6.3 Gateway cloning -- Chapter 6.4 Golden Gate Assembly for a modular cloning -- Chapter 6.5 In-Fusion Sequence and Ligation-Independent Cloning (In-Fusion SLIC) -- Chapter 6.6 T4 DNA Polymerase Sequence-and Ligation-Independent Cloning (T4 DNA Pol SLIC) -- Chapter 6.7 Non-template PCR cloning -- Part VII. Advice to cloners -- Chapter 7.1 When cloning is not going well -- Chapter 7.2 Keep your cloning data organized -- Appendix 1. Further readings -- Appendix 2. Abbreviations -- Appendix 3. Index.
520 $a This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.
650 0 $a Molecular cloning. $3 197743
650 0 $a Clone cells. $3 838860
650 1 4 $a Gene Expression. $3 220379
650 2 4 $a Genetic Engineering. $3 189027
650 2 4 $a Plant Genetics and Genomics. $3 790734
650 2 4 $a Animal Genetics and Genomics. $3 274341
700 1 $a Ro, Hyunju. $3 838858
700 1 $a Yi, Hankuil. $3 838859
710 2 $a SpringerLink (Online service) $3 273601
773 0 $t Springer eBooks
856 4 0 $u https://doi.org/10.1007/978-94-024-1662-6
950 $a Biomedical and Life Sciences (Springer-11642)