Language:
English
繁體中文
Help
圖資館首頁
Login
Back
to Search results for
[ subject:"Chemistry, Analytical." ]
Switch To:
Labeled
|
MARC Mode
|
ISBD
Open tubular capillary electrochroma...
~
Charles, Joseph Anthony Michael.
Open tubular capillary electrochromatography of isomeric dipeptides using a G-quartet forming DNA aptamer stationary phase.
Record Type:
Electronic resources : Monograph/item
Title/Author:
Open tubular capillary electrochromatography of isomeric dipeptides using a G-quartet forming DNA aptamer stationary phase.
Author:
Charles, Joseph Anthony Michael.
Description:
225 p.
Notes:
Adviser: Linda B. McGown.
Notes:
Source: Dissertation Abstracts International, Volume: 65-06, Section: B, page: 2910.
Contained By:
Dissertation Abstracts International65-06B.
Subject:
Chemistry, Analytical.
Online resource:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3135124
ISBN:
0496823485
Open tubular capillary electrochromatography of isomeric dipeptides using a G-quartet forming DNA aptamer stationary phase.
Charles, Joseph Anthony Michael.
Open tubular capillary electrochromatography of isomeric dipeptides using a G-quartet forming DNA aptamer stationary phase.
- 225 p.
Adviser: Linda B. McGown.
Thesis (Ph.D.)--Duke University, 2003.
Concentrated solutions of Guanosine (Guo) and Guanosine 5' -Monophosphate (GMP) were developed into gels. Gel formation was facilitated by the addition of certain alkaline metal ions and confirmed by visual inspection for increased viscosity and by CD spectroscopic studies. When the gel is formed individual nucleotides and nucleosides orient themselves into guanine-based coplanar tetrads or G-quartets. Like the G-quartet in tb-ligand, the guanine-based gels produce a circular dichroism spectrum that was used to monitor extent and stability of gel formation.
ISBN: 0496823485Subjects--Topical Terms:
224793
Chemistry, Analytical.
Open tubular capillary electrochromatography of isomeric dipeptides using a G-quartet forming DNA aptamer stationary phase.
LDR
:03127nmm _2200277 _450
001
162773
005
20051017073521.5
008
090528s2003 eng d
020
$a
0496823485
035
$a
00149274
040
$a
UnM
$c
UnM
100
0
$a
Charles, Joseph Anthony Michael.
$3
227917
245
1 0
$a
Open tubular capillary electrochromatography of isomeric dipeptides using a G-quartet forming DNA aptamer stationary phase.
300
$a
225 p.
500
$a
Adviser: Linda B. McGown.
500
$a
Source: Dissertation Abstracts International, Volume: 65-06, Section: B, page: 2910.
502
$a
Thesis (Ph.D.)--Duke University, 2003.
520
#
$a
Concentrated solutions of Guanosine (Guo) and Guanosine 5' -Monophosphate (GMP) were developed into gels. Gel formation was facilitated by the addition of certain alkaline metal ions and confirmed by visual inspection for increased viscosity and by CD spectroscopic studies. When the gel is formed individual nucleotides and nucleosides orient themselves into guanine-based coplanar tetrads or G-quartets. Like the G-quartet in tb-ligand, the guanine-based gels produce a circular dichroism spectrum that was used to monitor extent and stability of gel formation.
520
#
$a
DNA oligonucleotides that form intramolecular G-quartet structures were investigated as stationary phase reagents for separation of mixtures of isomeric dipeptides in open-tubular capillary electrochromatography (OTCEC). The oligonucleotides included a thrombin-binding aptamer (tb-ligand) that forms a biplanar G-quartet structure and an oligonucleotide that forms a 4-plane G-quartet structure. Fluorescence, circular dichroism and UV-visible absorbance spectroscopies were used in batch solution studies to detect interactions between the dipeptides and the biplanar G-quartet structure. Successful separations were achieved by OTCEC for a series of isomeric dipeptides. Results for OTCEC separations were compared with results obtained for capillary zone electrophoresis (CZE) separations on a bare, activated fused silica capillary. OTCEC with the aptamer stationary phase showed significant improvements in the resolution and reproducibility of CZE separations. In many studies, partial destabilization of the G-quartet structures by increased temperatures improved the separations and in some cases, different buffer systems were used in the OTCEC for enhanced separations.
520
#
$a
These gels were then developed into injectable monolithic stationary phases for CEC separations of a range of biomolecules based on their interactions with the gel matrix as well as the G-quartet structures. Separations were achieved for many of the isomeric dipeptides used in the first part of this work as well as for mixtures of homodipeptides, proteins and chiral drugs.
590
$a
School code: 0066.
650
# 0
$a
Chemistry, Analytical.
$3
224793
690
$a
0486
710
0 #
$a
Duke University.
$3
226880
773
0 #
$g
65-06B.
$t
Dissertation Abstracts International
790
$a
0066
790
1 0
$a
McGown, Linda B.,
$e
advisor
791
$a
Ph.D.
792
$a
2003
856
4 0
$u
http://libsw.nuk.edu.tw:81/login?url=http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3135124
$z
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3135124
based on 0 review(s)
ALL
電子館藏
Items
1 records • Pages 1 •
1
Inventory Number
Location Name
Item Class
Material type
Call number
Usage Class
Loan Status
No. of reservations
Opac note
Attachments
000000001266
電子館藏
1圖書
學位論文
一般使用(Normal)
On shelf
0
1 records • Pages 1 •
1
Multimedia
Multimedia file
http://libsw.nuk.edu.tw:81/login?url=http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3135124
Reviews
Add a review
and share your thoughts with other readers
Export
pickup library
Processing
...
Change password
Login