語系:
繁體中文
English
說明(常見問題)
圖資館首頁
登入
回首頁
到查詢結果
[ author_sort:"kawamoto, yusuke." ]
切換:
標籤
|
MARC模式
|
ISBD
Synthesis and biological evaluation ...
~
Kawamoto, Yusuke.
Synthesis and biological evaluation of pyrrole-imidazole polyamide probes for visualization of telomeres
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
Synthesis and biological evaluation of pyrrole-imidazole polyamide probes for visualization of telomeresby Yusuke Kawamoto.
作者:
Kawamoto, Yusuke.
出版者:
Singapore :Springer Singapore :2019.
面頁冊數:
xii, 122 p. :ill., digital ;24 cm.
Contained By:
Springer Nature eBook
標題:
Pyrroles.
電子資源:
https://doi.org/10.1007/978-981-13-6912-4
ISBN:
9789811369124$q(electronic bk.)
Synthesis and biological evaluation of pyrrole-imidazole polyamide probes for visualization of telomeres
Kawamoto, Yusuke.
Synthesis and biological evaluation of pyrrole-imidazole polyamide probes for visualization of telomeres
[electronic resource] /by Yusuke Kawamoto. - Singapore :Springer Singapore :2019. - xii, 122 p. :ill., digital ;24 cm. - Springer theses,2190-5053. - Springer theses..
1. General Introduction Sequence-specific DNA Binding Pyrrole-Imidazole Polyamides and Their Applications -- 2. Development of a New Method for Synthesis of Tandem Hairpin Pyrrole-Imidazole Polyamide Probes Targeting Human Telomeres -- 3. Tandem Trimer Pyrrole-Imidazole Polyamide Probes Targeting 18 Base Pairs in Human Telomere Sequences -- 4. Targeting 24 bp within Telomere Repeat Sequences with Tandem Tetramer Pyrrole?Imidazole Polyamide Probes.
This book presents a novel method of synthesizing sequence-specific DNA binding pyrrole-imidazole (Py-Im) polyamides and demonstrates polyamides targeting longer base pairs than those previously reported and their cellular application. It discusses the development of the facile synthetic method of tandem hairpin Py-Im polyamides and the method of visualizing telomeres under mild conditions without harsh denaturation, as well as research resulting in the synthesis not only of previously reported tandem dimer polyamides but also longer tandem trimer and tetramer polyamides. Using fluorescently labeled tandem hairpin Py-Im polyamides targeting a human telomere repetitive sequence, the author successfully stained telomeres in fixed cells without denaturation. These fluorescently labeled tandem tetramer polyamides are able to visualize human telomeres with lower background signals than previous polyamide probes, suggesting that they have higher specificity for telomeres. As such, this book provides insights into recent advance in Py-Im polyamides, particularly the extension of the target sites and improvement of the specificity, which can be applied to the study of pyrrole-imidazole polyamides with higher sequence specificity and the function of telomeres.
ISBN: 9789811369124$q(electronic bk.)
Standard No.: 10.1007/978-981-13-6912-4doiSubjects--Topical Terms:
878231
Pyrroles.
LC Class. No.: QD401 / .K38 2019
Dewey Class. No.: 547.593
Synthesis and biological evaluation of pyrrole-imidazole polyamide probes for visualization of telomeres
LDR
:02806nmm a2200337 a 4500
001
586674
003
DE-He213
005
20200701224728.0
006
m d
007
cr nn 008maaau
008
210326s2019 si s 0 eng d
020
$a
9789811369124$q(electronic bk.)
020
$a
9789811369117$q(paper)
024
7
$a
10.1007/978-981-13-6912-4
$2
doi
035
$a
978-981-13-6912-4
040
$a
GP
$c
GP
041
0
$a
eng
050
4
$a
QD401
$b
.K38 2019
072
7
$a
PSB
$2
bicssc
072
7
$a
SCI013040
$2
bisacsh
072
7
$a
PSB
$2
thema
082
0 4
$a
547.593
$2
23
090
$a
QD401
$b
.K22 2019
100
1
$a
Kawamoto, Yusuke.
$3
878230
245
1 0
$a
Synthesis and biological evaluation of pyrrole-imidazole polyamide probes for visualization of telomeres
$h
[electronic resource] /
$c
by Yusuke Kawamoto.
260
$a
Singapore :
$b
Springer Singapore :
$b
Imprint: Springer,
$c
2019.
300
$a
xii, 122 p. :
$b
ill., digital ;
$c
24 cm.
490
1
$a
Springer theses,
$x
2190-5053
505
0
$a
1. General Introduction Sequence-specific DNA Binding Pyrrole-Imidazole Polyamides and Their Applications -- 2. Development of a New Method for Synthesis of Tandem Hairpin Pyrrole-Imidazole Polyamide Probes Targeting Human Telomeres -- 3. Tandem Trimer Pyrrole-Imidazole Polyamide Probes Targeting 18 Base Pairs in Human Telomere Sequences -- 4. Targeting 24 bp within Telomere Repeat Sequences with Tandem Tetramer Pyrrole?Imidazole Polyamide Probes.
520
$a
This book presents a novel method of synthesizing sequence-specific DNA binding pyrrole-imidazole (Py-Im) polyamides and demonstrates polyamides targeting longer base pairs than those previously reported and their cellular application. It discusses the development of the facile synthetic method of tandem hairpin Py-Im polyamides and the method of visualizing telomeres under mild conditions without harsh denaturation, as well as research resulting in the synthesis not only of previously reported tandem dimer polyamides but also longer tandem trimer and tetramer polyamides. Using fluorescently labeled tandem hairpin Py-Im polyamides targeting a human telomere repetitive sequence, the author successfully stained telomeres in fixed cells without denaturation. These fluorescently labeled tandem tetramer polyamides are able to visualize human telomeres with lower background signals than previous polyamide probes, suggesting that they have higher specificity for telomeres. As such, this book provides insights into recent advance in Py-Im polyamides, particularly the extension of the target sites and improvement of the specificity, which can be applied to the study of pyrrole-imidazole polyamides with higher sequence specificity and the function of telomeres.
650
0
$a
Pyrroles.
$3
878231
650
0
$a
Imidazoles.
$3
878232
650
1 4
$a
Bioorganic Chemistry.
$3
275016
650
2 4
$a
Nucleic Acid Chemistry.
$3
376665
710
2
$a
SpringerLink (Online service)
$3
273601
773
0
$t
Springer Nature eBook
830
0
$a
Springer theses.
$3
557607
856
4 0
$u
https://doi.org/10.1007/978-981-13-6912-4
950
$a
Chemistry and Materials Science (SpringerNature-11644)
筆 0 讀者評論
全部
電子館藏
館藏
1 筆 • 頁數 1 •
1
條碼號
館藏地
館藏流通類別
資料類型
索書號
使用類型
借閱狀態
預約狀態
備註欄
附件
000000190459
電子館藏
1圖書
電子書
EB QD401 .K22 2019 2019
一般使用(Normal)
在架
0
1 筆 • 頁數 1 •
1
多媒體
多媒體檔案
https://doi.org/10.1007/978-981-13-6912-4
評論
新增評論
分享你的心得
Export
取書館別
處理中
...
變更密碼
登入