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Molecular protocols in transfusion m...

Denomme, Gregory A.

Molecular protocols in transfusion medicine

Record Type:	Electronic resources : Monograph/item
Title/Author:	Molecular protocols in transfusion medicineGregory A. Denomme, Maria Rios, Marion E. Reid.
Author:	Denomme, Gregory A.
other author:	Rios, Maria.
Published:	San Diego, CA :Academic Press,2000.
Description:	x, 186 p. :ill. ;24 cm.
Subject:	Blood groupsHandbooks, manuals, etc.
Online resource:	An electronic book accessible through the World Wide Web; click for information
ISBN:	9780122093708

Molecular protocols in transfusion medicine
Denomme, Gregory A.

Molecular protocols in transfusion medicine[electronic resource] /Gregory A. Denomme, Maria Rios, Marion E. Reid. - San Diego, CA :Academic Press,2000. - x, 186 p. :ill. ;24 cm.

Includes bibliographical references and index.

This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines. Key Features * Essential for hematologists and those involoved in tissue typing and the study of human genetic polymorphisms * Presents clearly and concisely the information on a particular variant and the technique used to detect it * Organized by antigen and provides sequences of polymorphisms and primers * Details the general concepts and critical information on genes, their products, and sources of relevant nucleic acids * Includes protocols that allow investigators to set up assays with minimal effort (protocols include primers, reagents, reaction conditions, sizes of amplified products, restriction fragment digests, and the relevant safety information) * Provides information that helps interpret results in clinical settings * Contains additional sources of information (e.g., key references, web site addresses, glossary, Database accession numbers, request forms, and worksheets for PCR/enzyme digests).

Electronic reproduction.
Amsterdam :
Elsevier Science & Technology,
2007.

Mode of access: World Wide Web.

ISBN: 9780122093708

Source: 88709:88709Elsevier Science & Technologyhttp://www.sciencedirect.comSubjects--Topical Terms:

504707
Blood groups
--Handbooks, manuals, etc.Index Terms--Genre/Form:

214472
Electronic books.

LC Class. No.: QP98 / .D46 2000eb

Dewey Class. No.: 612.1/1825

National Library of Medicine Call No.: 2000 H-750

Molecular protocols in transfusion medicine
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245 1 0 $a Molecular protocols in transfusion medicine $h [electronic resource] / $c Gregory A. Denomme, Maria Rios, Marion E. Reid.
260 $a San Diego, CA : $b Academic Press, $c 2000.
300 $a x, 186 p. : $b ill. ; $c 24 cm.
504 $a Includes bibliographical references and index.
520 $a This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines. Key Features * Essential for hematologists and those involoved in tissue typing and the study of human genetic polymorphisms * Presents clearly and concisely the information on a particular variant and the technique used to detect it * Organized by antigen and provides sequences of polymorphisms and primers * Details the general concepts and critical information on genes, their products, and sources of relevant nucleic acids * Includes protocols that allow investigators to set up assays with minimal effort (protocols include primers, reagents, reaction conditions, sizes of amplified products, restriction fragment digests, and the relevant safety information) * Provides information that helps interpret results in clinical settings * Contains additional sources of information (e.g., key references, web site addresses, glossary, Database accession numbers, request forms, and worksheets for PCR/enzyme digests).
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650 0 $a Genetic polymorphisms $v Handbooks, manuals, etc. $3 504708
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