國立高雄大學圖資館 |

語系: 繁體中文

說明(常見問題)

圖資館首頁

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

pflp轉殖水稻對白葉枯病的抗病性來自於增強蔗糖轉化酶與六磷酸激酶活性 ...

國立高雄大學生物科技研究所碩士班

pflp轉殖水稻對白葉枯病的抗病性來自於增強蔗糖轉化酶與六磷酸激酶活性 = Disease resistance of pflp-transgenic rice against bacterial blight results from enhancement of invertase and hexokinase activities

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Disease resistance of pflp-transgenic rice against bacterial blight results from enhancement of invertase and hexokinase activities
作者:	張雅雲,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	2013[民102]
面頁冊數:	42葉部分彩圖，表格 : 30公分;
標題:	植物硫鐵蛋白
標題:	plant ferredoxin-like protein (PFLP)
電子資源:	http://hdl.handle.net/11296/ndltd/00352805556825186963
附註:	106年10月31日公開
附註:	參考書目：葉36-42
摘要註:	醣類為植物生長發育過程的重要物質，不僅可作為代謝能量來源，同時也是一種訊息傳遞分子。當植物遭受病原入侵時，蔗糖轉化酶(invertase，INV)可將蔗糖水解成果糖與葡萄糖，葡萄糖即可透過六磷酸激酶(hexokinase，HXK)調控粒線體中的過氧化物(reactive oxygen species，ROS)生成。ROS作為一訊息傳遞分子，可誘發植物體內包括細胞壁增厚，產生植物防禦素(phytoalexins)與病程相關蛋白(pathogenesis-related proteins，PR)等抗病反應。植物硫鐵蛋白相似蛋白(Plant ferredoxin like protein，PFLP)分離自甜椒，與阿拉伯芥之第一型硫鐵蛋白有高度相似性。第一型硫鐵蛋白廣泛分布在植物綠色組織中，其主要在光合作用電子傳遞鏈中扮演傳遞電子的角色。先前研究已知過表現PFLP之轉殖水稻具有較高的電子傳遞能力與光合作用效率，使轉殖水稻含有較高的醣類累積與HXK基因表現量。許多研究也指出PFLP轉殖植物可透過ROS的產生使植物對抗病原性的侵害，但醣類相關酵素在此抗病反應中所參與的作用機制尚未明瞭。因此本篇研究將探討蔗糖轉化酶與六磷酸激酶在pflp轉殖水稻於白葉枯病抗病上所扮演的角色。實驗結果顯示轉殖水稻對白葉枯病有較高的抗病性，而其在感病初期PR蛋白的誘發程度亦較台農67號水稻(TNG67)高。在感染白葉枯病病原菌時轉殖水稻能表現較TNG67高2倍量的cwINV，以此累積比TNG67高1.6倍的葡萄糖含量，並透過葡萄糖分別提升HXK2與HXK6約2倍與2.5倍的基因表現量，在ROS生成方面，轉殖水稻在前期可產生較TNG67約2.5倍高的ROS，使轉殖水稻引發較高量的ROS誘導抗病系統抑制病原進一步發展。綜合上述結果，本篇研究指出PFLP轉殖水稻可以透過INV累積較高量的葡萄糖並透過此來活化HXK，以在病原感染初期即產生ROS使之作訊號傳遞，表現PR蛋白等相關抗病基因來增進轉殖水稻對白葉枯病的抗性。 Sugar is an important biological compound for plant growth and development. Not only serves as important energy source but also acts as signaling molecule in plants. Invertase (INV) can be hydrolysis of sucrose to fructose and glucose during pathogen infection. Glucose signal regulates reactive oxygen species (ROS) generation through mitochondrial hexokinase (HXK) during pathogen infection. As signals, ROS induces several defense responses including cell wall thickening, phytoalexin synthesis and pathogenesis-related (PR) proteins transcription. The plant ferredoxin - like protein (PFLP), isolated from sweet peppers, shows high homology to sequence of Fd-I, an electron carrier in photosynthesis. Previous studies reported that constitutive expression of pflp in transgenic plants exhibited highly photosynthesis efficiency, increased soluble sugars contents, and altered HXK expression pattern. Many studies also have been reported that continuously expression of PFLP in transgenic plants encourages the generation of ROS and participates in pathogen-resistant mechanism. However, the function of sugar associated enzyme involved in pathogen-resistant mechanism is unclear. This study is to investigate the role of INV and HXK in pflp transgenic plants resistant to bacterial blight. In this report, we demonstrated that PFLP can enhance disease resistance to bacterial blight and quickly induce PR protein transcription in early phase of pathogen infection. Additionally, the expressions of cwINV in transgenic plants showed 2-folds higher than TNG67 after Xoo infection, and accumulated glucose content 1.6 times higher than TNG67 in transgenic plants. This result was due to increased HXK2 and HXK6 2-fold and 2.5-fold gene expression pattern. In ROS generation, the transgenic rice generated ROS about 2.5 times higher than TNG67 in early Xoo infection phase. Generated ROS induced defense responses of against bacterial blight. Altogether, we demonstrated PFLP transgenic rice plants accumulated higher levels of glucose via INV and tolerance against bacterial blight was improved through glucose activation HXK, which regulated ROS production in pathogen infection early phase.

pflp轉殖水稻對白葉枯病的抗病性來自於增強蔗糖轉化酶與六磷酸激酶活性 = Disease resistance of pflp-transgenic rice against bacterial blight results from enhancement of invertase and hexokinase activities
張, 雅雲

pflp轉殖水稻對白葉枯病的抗病性來自於增強蔗糖轉化酶與六磷酸激酶活性 = Disease resistance of pflp-transgenic rice against bacterial blight results from enhancement of invertase and hexokinase activities / 張雅雲撰 - [高雄市] : 撰者, 2013[民102]. - 42葉 ; 部分彩圖，表格 ; 30公分.
106年10月31日公開參考書目：葉36-42.
植物硫鐵蛋白plant ferredoxin-like protein (PFLP)

pflp轉殖水稻對白葉枯病的抗病性來自於增強蔗糖轉化酶與六磷酸激酶活性 = Disease resistance of pflp-transgenic rice against bacterial blight results from enhancement of invertase and hexokinase activities
LDR:05589nam0 2200277 450 001 389519
005 20171103092756.0
010 0 $b 精裝
010 0 $b 平裝
100 $a 20130926y2013 k y0chiy50 e
101 0 $a eng $d eng $d chi
102 $a tw
105 $a ak am 000yy
200 1 $a pflp轉殖水稻對白葉枯病的抗病性來自於增強蔗糖轉化酶與六磷酸激酶活性 $d Disease resistance of pflp-transgenic rice against bacterial blight results from enhancement of invertase and hexokinase activities $f 張雅雲撰
210 $a [高雄市] $c 撰者 $d 2013[民102]
215 0 $a 42葉 $c 部分彩圖，表格 $d 30公分
300 $a 106年10月31日公開
300 $a 參考書目：葉36-42
314 $a 指導教授：葛孟杰博士
328 $a 碩士論文--國立高雄大學生物科技研究所
330 $a 醣類為植物生長發育過程的重要物質，不僅可作為代謝能量來源，同時也是一種訊息傳遞分子。當植物遭受病原入侵時，蔗糖轉化酶(invertase，INV)可將蔗糖水解成果糖與葡萄糖，葡萄糖即可透過六磷酸激酶(hexokinase，HXK)調控粒線體中的過氧化物(reactive oxygen species，ROS)生成。ROS作為一訊息傳遞分子，可誘發植物體內包括細胞壁增厚，產生植物防禦素(phytoalexins)與病程相關蛋白(pathogenesis-related proteins，PR)等抗病反應。植物硫鐵蛋白相似蛋白(Plant ferredoxin like protein，PFLP)分離自甜椒，與阿拉伯芥之第一型硫鐵蛋白有高度相似性。第一型硫鐵蛋白廣泛分布在植物綠色組織中，其主要在光合作用電子傳遞鏈中扮演傳遞電子的角色。先前研究已知過表現PFLP之轉殖水稻具有較高的電子傳遞能力與光合作用效率，使轉殖水稻含有較高的醣類累積與HXK基因表現量。許多研究也指出PFLP轉殖植物可透過ROS的產生使植物對抗病原性的侵害，但醣類相關酵素在此抗病反應中所參與的作用機制尚未明瞭。因此本篇研究將探討蔗糖轉化酶與六磷酸激酶在pflp轉殖水稻於白葉枯病抗病上所扮演的角色。實驗結果顯示轉殖水稻對白葉枯病有較高的抗病性，而其在感病初期PR蛋白的誘發程度亦較台農67號水稻(TNG67)高。在感染白葉枯病病原菌時轉殖水稻能表現較TNG67高2倍量的cwINV，以此累積比TNG67高1.6倍的葡萄糖含量，並透過葡萄糖分別提升HXK2與HXK6約2倍與2.5倍的基因表現量，在ROS生成方面，轉殖水稻在前期可產生較TNG67約2.5倍高的ROS，使轉殖水稻引發較高量的ROS誘導抗病系統抑制病原進一步發展。綜合上述結果，本篇研究指出PFLP轉殖水稻可以透過INV累積較高量的葡萄糖並透過此來活化HXK，以在病原感染初期即產生ROS使之作訊號傳遞，表現PR蛋白等相關抗病基因來增進轉殖水稻對白葉枯病的抗性。 Sugar is an important biological compound for plant growth and development. Not only serves as important energy source but also acts as signaling molecule in plants. Invertase (INV) can be hydrolysis of sucrose to fructose and glucose during pathogen infection. Glucose signal regulates reactive oxygen species (ROS) generation through mitochondrial hexokinase (HXK) during pathogen infection. As signals, ROS induces several defense responses including cell wall thickening, phytoalexin synthesis and pathogenesis-related (PR) proteins transcription. The plant ferredoxin - like protein (PFLP), isolated from sweet peppers, shows high homology to sequence of Fd-I, an electron carrier in photosynthesis. Previous studies reported that constitutive expression of pflp in transgenic plants exhibited highly photosynthesis efficiency, increased soluble sugars contents, and altered HXK expression pattern. Many studies also have been reported that continuously expression of PFLP in transgenic plants encourages the generation of ROS and participates in pathogen-resistant mechanism. However, the function of sugar associated enzyme involved in pathogen-resistant mechanism is unclear. This study is to investigate the role of INV and HXK in pflp transgenic plants resistant to bacterial blight. In this report, we demonstrated that PFLP can enhance disease resistance to bacterial blight and quickly induce PR protein transcription in early phase of pathogen infection. Additionally, the expressions of cwINV in transgenic plants showed 2-folds higher than TNG67 after Xoo infection, and accumulated glucose content 1.6 times higher than TNG67 in transgenic plants. This result was due to increased HXK2 and HXK6 2-fold and 2.5-fold gene expression pattern. In ROS generation, the transgenic rice generated ROS about 2.5 times higher than TNG67 in early Xoo infection phase. Generated ROS induced defense responses of against bacterial blight. Altogether, we demonstrated PFLP transgenic rice plants accumulated higher levels of glucose via INV and tolerance against bacterial blight was improved through glucose activation HXK, which regulated ROS production in pathogen infection early phase.
510 1 $a Disease resistance of pflp-transgenic rice against bacterial blight results from enhancement of invertase and hexokinase activities
610 # 0 $a 植物硫鐵蛋白 $a 白葉枯病 $a 蔗糖轉化酶 $a 六磷酸激酶
610 # 1 $a plant ferredoxin-like protein (PFLP) $a bacterial blight $a invertase $a hexokinase
681 $a 008M/0019 $b 420228 1171 $v 2007年版
700 1 $a 張 $b 雅雲 $4 撰 $3 614400
712 0 2 $a 國立高雄大學 $b 生物科技研究所碩士班 $3 593057
801 0 $a tw $b NUK $c 20171030 $g CCR
856 7 # $u http://hdl.handle.net/11296/ndltd/00352805556825186963 $2 http $z 電子資源