國立高雄大學圖資館 |

語系: 繁體中文

說明(常見問題)

圖資館首頁

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Recombinant enzymesfrom basic scienc...

Amid, Azura.

Recombinant enzymesfrom basic science to commercialization /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Recombinant enzymesedited by Azura Amid.
其他題名:	from basic science to commercialization /
其他作者:	Amid, Azura.
出版者:	Cham :Springer International Publishing :2015.
面頁冊數:	x, 188 p. :ill., digital ;24 cm.
Contained By:	Springer eBooks
標題:	Enzymes.
電子資源:	http://dx.doi.org/10.1007/978-3-319-12397-4
ISBN:	9783319123974 (electronic bk.)

Recombinant enzymesfrom basic science to commercialization /
Recombinant enzymesfrom basic science to commercialization /[electronic resource] :edited by Azura Amid. - Cham :Springer International Publishing :2015. - x, 188 p. :ill., digital ;24 cm.

Introduction to Recombinant Enzyme Pre-commercialization -- Recombinant Enzyme: Cloning and Expression -- Common Laboratory Procedure -- Characterization of Recombinant Enzymes -- Purification of Recombinant Protein for Industrial Use -- Recombinant Enzyme Fermentation -- Scaling-up Recombinant Enzyme Fermentation -- Downstream Processing of Recombinant Enzymes for Commercialization -- Economic and Environmental Evaluation of Recombinant Enzyme Production -- Case Study: Recombinant Bromelain Selection -- Case Study: Recombinant Bromelain Cloning, Characterization and Upstream Processes -- Case Study: Recombinant Bromelain Downstream Processing.

This edited work presents studies that clarify the basics of producing recombinant enzymes that finally lead to commercialization. It enables researchers to see what is crucial to the commercialization process, from examining the cloning method, using analytical techniques such as calculating the total protein content and enzyme activity, through considering upstream and downstream processes, to the final product. Readers will discover the importance of the cloning method as it influences the upstream and downstream processes and determines the level of success of the recombinant enzyme commercialization processes. We see that the two main factors that are particularly sensitive during the cloning process are the vector and the host. A discussion of analytical techniques is presented followed by studies on important stages during the upstream processes including the process of optimizing the media to get results and high enzyme activity. Downstream processes such as the cell disruption technique, purification and formulation of the final product are then considered. The reader is introduced to software that helps streamline recombinant enzyme production from the upstream to downstream processes, to facilitate the process of up-scaling production. This work includes a case study as tool, to guide understanding of the commercialization process. The work is written for researchers in the field and is especially suited to those who are under pressure to embark on the tough process of commercialization.

ISBN: 9783319123974 (electronic bk.)

Standard No.: 10.1007/978-3-319-12397-4doiSubjects--Topical Terms:

193013
Enzymes.

LC Class. No.: TP248.E5

Dewey Class. No.: 572.7

Recombinant enzymesfrom basic science to commercialization /
LDR:03181nmm a2200337 a 4500 001 461298
003 DE-He213
005 20150819112130.0
006 m d
007 cr nn 008maaau
008 151110s2015 gw s 0 eng d
020 $a 9783319123974 (electronic bk.)
020 $a 9783319123967 (paper)
024 7 $a 10.1007/978-3-319-12397-4 $2 doi
035 $a 978-3-319-12397-4
040 $a GP $c GP
041 0 $a eng
050 4 $a TP248.E5
072 7 $a PS $2 bicssc
072 7 $a PDN $2 bicssc
072 7 $a SCI008000 $2 bisacsh
072 7 $a SCI043000 $2 bisacsh
082 0 4 $a 572.7 $2 23
090 $a TP248.E5 $b R311 2015
245 0 0 $a Recombinant enzymes $h [electronic resource] : $b from basic science to commercialization / $c edited by Azura Amid.
260 $a Cham : $b Springer International Publishing : $b Imprint: Springer, $c 2015.
300 $a x, 188 p. : $b ill., digital ; $c 24 cm.
505 0 $a Introduction to Recombinant Enzyme Pre-commercialization -- Recombinant Enzyme: Cloning and Expression -- Common Laboratory Procedure -- Characterization of Recombinant Enzymes -- Purification of Recombinant Protein for Industrial Use -- Recombinant Enzyme Fermentation -- Scaling-up Recombinant Enzyme Fermentation -- Downstream Processing of Recombinant Enzymes for Commercialization -- Economic and Environmental Evaluation of Recombinant Enzyme Production -- Case Study: Recombinant Bromelain Selection -- Case Study: Recombinant Bromelain Cloning, Characterization and Upstream Processes -- Case Study: Recombinant Bromelain Downstream Processing.
520 $a This edited work presents studies that clarify the basics of producing recombinant enzymes that finally lead to commercialization. It enables researchers to see what is crucial to the commercialization process, from examining the cloning method, using analytical techniques such as calculating the total protein content and enzyme activity, through considering upstream and downstream processes, to the final product. Readers will discover the importance of the cloning method as it influences the upstream and downstream processes and determines the level of success of the recombinant enzyme commercialization processes. We see that the two main factors that are particularly sensitive during the cloning process are the vector and the host. A discussion of analytical techniques is presented followed by studies on important stages during the upstream processes including the process of optimizing the media to get results and high enzyme activity. Downstream processes such as the cell disruption technique, purification and formulation of the final product are then considered. The reader is introduced to software that helps streamline recombinant enzyme production from the upstream to downstream processes, to facilitate the process of up-scaling production. This work includes a case study as tool, to guide understanding of the commercialization process. The work is written for researchers in the field and is especially suited to those who are under pressure to embark on the tough process of commercialization.
650 0 $a Enzymes. $3 193013
650 0 $a Recombinant DNA. $3 197744
650 1 4 $a Life Sciences. $3 273679
650 2 4 $a Biological Techniques. $3 276128
650 2 4 $a Industrial and Production Engineering. $3 273753
650 2 4 $a Biomedicine general. $3 273948
700 1 $a Amid, Azura. $3 713293
710 2 $a SpringerLink (Online service) $3 273601
773 0 $t Springer eBooks
856 4 0 $u http://dx.doi.org/10.1007/978-3-319-12397-4
950 $a Biomedical and Life Sciences (Springer-11642)