國立高雄大學圖資館 |

語系: 繁體中文

說明(常見問題)

圖資館首頁

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Development of a fully integrated "S...

SpringerLink (Online service)

Development of a fully integrated "Sample-in-answer-out" system for automatic genetic analysis

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Development of a fully integrated "Sample-in-answer-out" system for automatic genetic analysisby Bin Zhuang.
作者:	Zhuang, Bin.
出版者:	Singapore :Springer Singapore :2018.
面頁冊數:	xxiii, 114 p. :ill., digital ;24 cm.
Contained By:	Springer eBooks
標題:	DNAAnalysis.
電子資源:	http://dx.doi.org/10.1007/978-981-10-4753-4
ISBN:	9789811047534$q(electronic bk.)

Development of a fully integrated "Sample-in-answer-out" system for automatic genetic analysis
Zhuang, Bin.

Development of a fully integrated "Sample-in-answer-out" system for automatic genetic analysis[electronic resource] /by Bin Zhuang. - Singapore :Springer Singapore :2018. - xxiii, 114 p. :ill., digital ;24 cm. - Springer theses,2190-5053. - Springer theses..

Introduction -- The construction of a general platform for capillary electrophoresis -- Integrated module for automatic DNA extraction and amplification -- A fully-integrated genetic analysis system -- Conclusion and prospects for future work.

This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an "in-situ" PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system's performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid "sample-in-answer-out" testing in routine clinical practice.

ISBN: 9789811047534$q(electronic bk.)

Standard No.: 10.1007/978-981-10-4753-4doiSubjects--Topical Terms:

194476
DNA
--Analysis.

LC Class. No.: QP624

Dewey Class. No.: 572.8

Development of a fully integrated "Sample-in-answer-out" system for automatic genetic analysis
LDR:02384nmm a2200325 a 4500 001 526921
003 DE-He213
005 20170720143515.0
006 m d
007 cr nn 008maaau
008 181015s2018 si s 0 eng d
020 $a 9789811047534$q(electronic bk.)
020 $a 9789811047527$q(paper)
024 7 $a 10.1007/978-981-10-4753-4 $2 doi
035 $a 978-981-10-4753-4
040 $a GP $c GP
041 0 $a eng
050 4 $a QP624
072 7 $a MQW $2 bicssc
072 7 $a TEC009000 $2 bisacsh
082 0 4 $a 572.8 $2 23
090 $a QP624 $b .Z63 2018
100 1 $a Zhuang, Bin. $3 799538
245 1 0 $a Development of a fully integrated "Sample-in-answer-out" system for automatic genetic analysis $h [electronic resource] / $c by Bin Zhuang.
260 $a Singapore : $b Springer Singapore : $b Imprint: Springer, $c 2018.
300 $a xxiii, 114 p. : $b ill., digital ; $c 24 cm.
490 1 $a Springer theses, $x 2190-5053
505 0 $a Introduction -- The construction of a general platform for capillary electrophoresis -- Integrated module for automatic DNA extraction and amplification -- A fully-integrated genetic analysis system -- Conclusion and prospects for future work.
520 $a This thesis reports on the development of a fully integrated and automated microsystem consisting of low-cost, disposable plastic chips for DNA extraction and PCR amplification, combined with a reusable glass capillary array electrophoresis chip, which can be employed in a modular-based format for genetic analysis. In the thesis, DNA extraction is performed by adopting a filter paper-based method, followed by an "in-situ" PCR carried out directly in the same reaction chamber of the chip without elution. PCR products are then co-injected with sizing standards into separation channels for detection using a novel injection electrode. The entire process is automatically carried out by a custom-made compact control and detection instrument. The author thoroughly tests the system's performance and reliability by conducting rapid genetic screening of mutations on congenital hearing loss and pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. The successful development and operation of this microsystem establishes the feasibility of rapid "sample-in-answer-out" testing in routine clinical practice.
650 0 $a DNA $x Analysis. $3 194476
650 0 $a Microfluidics. $3 242354
650 1 4 $a Engineering. $3 210888
650 2 4 $a Biomedical Engineering. $3 190464
650 2 4 $a Genetic Engineering. $3 189027
650 2 4 $a Nucleic Acid Chemistry. $3 376665
710 2 $a SpringerLink (Online service) $3 273601
773 0 $t Springer eBooks
830 0 $a Springer theses. $3 557607
856 4 0 $u http://dx.doi.org/10.1007/978-981-10-4753-4
950 $a Engineering (Springer-11647)