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X-Ray diffraction imaging of biologi...

Nakasako, Masayoshi.

X-Ray diffraction imaging of biological cells

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	X-Ray diffraction imaging of biological cellsby Masayoshi Nakasako.
作者:	Nakasako, Masayoshi.
出版者:	Tokyo :Springer Japan :2018.
面頁冊數:	xx, 228 p. :ill. (some col.), digital ;24 cm.
Contained By:	Springer eBooks
標題:	X-raysDiffraction.
電子資源:	http://dx.doi.org/10.1007/978-4-431-56618-2
ISBN:	9784431566182$q(electronic bk.)

X-Ray diffraction imaging of biological cells
Nakasako, Masayoshi.

X-Ray diffraction imaging of biological cells[electronic resource] /by Masayoshi Nakasako. - Tokyo :Springer Japan :2018. - xx, 228 p. :ill. (some col.), digital ;24 cm. - Springer series in optical sciences,v.2100342-4111 ;. - Springer series in optical sciences ;v. 93..

Introduction -- X-ray diffraction -- Theory of X-ray diffraction imaging -- Diffraction apparatus for X-ray diffraction imaging -- Specimen preparation for X-ray diffraction imaging experiments at cryogenic temperature -- Processing of diffraction patterns obtained from X-ray diffraction imaging experiments using X-ray free electron laser pulses -- Phase retrieval of diffraction patterns -- Projection structures of biological cells and organelles -- Three-dimensional structural analyses in cryogenic X-ray diffraction imaging -- Prospects for the structural analysis of biological specimens by X-ray diffraction imaging.

In this book, the author describes the development of the experimental diffraction setup and structural analysis of non-crystalline particles from material science and biology. Recent advances in X-ray free electron laser (XFEL)-coherent X-ray diffraction imaging (CXDI) experiments allow for the structural analysis of non-crystalline particles to a resolution of 7 nm, and to a resolution of 20 nm for biological materials. Now XFEL-CXDI marks the dawn of a new era in structural analys of non-crystalline particles with dimensions larger than 100 nm, which was quite impossible in the 20th century. To conduct CXDI experiments in both synchrotron and XFEL facilities, the author has developed apparatuses, named KOTOBUKI-1 and TAKASAGO-6 for cryogenic diffraction experiments on frozen-hydrated non-crystalline particles at around 66 K. At the synchrotron facility, cryogenic diffraction experiments dramatically reduce radiation damage of specimen particles and allow tomography CXDI experiments. In addition, in XFEL experiments, non-crystalline particles scattered on thin support membranes and flash-cooled can be used to efficiently increase the rate of XFEL pulses. The rate, which depends on the number density of scattered particles and the size of X-ray beams, is currently 20-90%, probably the world record in XFEL-CXDI experiments. The experiment setups and results are introduced in this book. The author has also developed software suitable for efficiently processing of diffraction patterns and retrieving electron density maps of specimen particles based on the diffraction theory used in CXDI.

ISBN: 9784431566182$q(electronic bk.)

Standard No.: 10.1007/978-4-431-56618-2doiSubjects--Topical Terms:

208566
X-rays
--Diffraction.

LC Class. No.: QC482.D5 / N353 2018

Dewey Class. No.: 539.7222

X-Ray diffraction imaging of biological cells
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520 $a In this book, the author describes the development of the experimental diffraction setup and structural analysis of non-crystalline particles from material science and biology. Recent advances in X-ray free electron laser (XFEL)-coherent X-ray diffraction imaging (CXDI) experiments allow for the structural analysis of non-crystalline particles to a resolution of 7 nm, and to a resolution of 20 nm for biological materials. Now XFEL-CXDI marks the dawn of a new era in structural analys of non-crystalline particles with dimensions larger than 100 nm, which was quite impossible in the 20th century. To conduct CXDI experiments in both synchrotron and XFEL facilities, the author has developed apparatuses, named KOTOBUKI-1 and TAKASAGO-6 for cryogenic diffraction experiments on frozen-hydrated non-crystalline particles at around 66 K. At the synchrotron facility, cryogenic diffraction experiments dramatically reduce radiation damage of specimen particles and allow tomography CXDI experiments. In addition, in XFEL experiments, non-crystalline particles scattered on thin support membranes and flash-cooled can be used to efficiently increase the rate of XFEL pulses. The rate, which depends on the number density of scattered particles and the size of X-ray beams, is currently 20-90%, probably the world record in XFEL-CXDI experiments. The experiment setups and results are introduced in this book. The author has also developed software suitable for efficiently processing of diffraction patterns and retrieving electron density maps of specimen particles based on the diffraction theory used in CXDI.
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