國立高雄大學圖資館 |

Language: English

Back

Molecular dynamics study of hydrated 1,2-dilignoceroylphosphatidylcholine (DLGPC) monolayer and virus protein U (Vpu)/membrane/water systems.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Molecular dynamics study of hydrated 1,2-dilignoceroylphosphatidylcholine (DLGPC) monolayer and virus protein U (Vpu)/membrane/water systems.
Author:	Sun, Feng.
Description:	186 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-04, Section: B, page: 1744.
Notes:	Supervisor: Walter Licht.
Contained By:	Dissertation Abstracts International64-04B.
Subject:	Chemistry, Physical.
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3087472
ISBN:	0496352423

Molecular dynamics study of hydrated 1,2-dilignoceroylphosphatidylcholine (DLGPC) monolayer and virus protein U (Vpu)/membrane/water systems.
Sun, Feng.

Molecular dynamics study of hydrated 1,2-dilignoceroylphosphatidylcholine (DLGPC) monolayer and virus protein U (Vpu)/membrane/water systems. [electronic resource] - 186 p.

Source: Dissertation Abstracts International, Volume: 64-04, Section: B, page: 1744.

Thesis (Ph.D.)--University of Pennsylvania, 2003.

Constant normal pressure, constant surface tension and constant temperature (NPNgammaT) molecular dynamics (MD) simulations have been performed on 1,2-dilignoceroylphosphatidylcholine (DLGPC) Langmuir monolayer and Virus Protein U (Vpu)/DLGPC/water monolayer systems. NPNgammaT ensemble was employed to reproduce the experimental observations, such as area/lipid, since surface tension is an essential factor in determining the monolayer structure. Data analysis on DLGPC/water monolayer shows that various liquid-condensed phase properties of the monolayer have been well reproduced from the simulation, indicating that surface tension 22.9mN/m used in the simulation is an appropriate condition for the condensed phase NPNgammaT simulation. The simulation results suggest that this long-chain phospholipid monolayer shares many structural characteristics with typical short-chain 1,2-diacylphosphatidylcholine systems. Furthermore, it was found that DLGPC/water monolayer has almost completely rotationally disordered acyl chains which have not been observed so far in short-chain 1,2-diacylphosphatidylcholine/water bilayers. The simulation indicates the good biological relevance of the DLGPC/water monolayer which might be useful in protein/lipid studies to reveal protein structure and protein-lipid interactions in a membrane environment.

ISBN: 0496352423Subjects--Topical Terms:

226924
Chemistry, Physical.

Molecular dynamics study of hydrated 1,2-dilignoceroylphosphatidylcholine (DLGPC) monolayer and virus protein U (Vpu)/membrane/water systems.
LDR:03652nmm _2200265 _450 001 161944
005 20051017073354.5
008 230606s2003 eng d
020 $a 0496352423
035 $a 00148445
035 $a 161944
040 $a UnM $c UnM
100 0 $a Sun, Feng. $3 227039
245 1 0 $a Molecular dynamics study of hydrated 1,2-dilignoceroylphosphatidylcholine (DLGPC) monolayer and virus protein U (Vpu)/membrane/water systems. $h [electronic resource]
300 $a 186 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-04, Section: B, page: 1744.
500 $a Supervisor: Walter Licht.
502 $a Thesis (Ph.D.)--University of Pennsylvania, 2003.
520 # $a Constant normal pressure, constant surface tension and constant temperature (NPNgammaT) molecular dynamics (MD) simulations have been performed on 1,2-dilignoceroylphosphatidylcholine (DLGPC) Langmuir monolayer and Virus Protein U (Vpu)/DLGPC/water monolayer systems. NPNgammaT ensemble was employed to reproduce the experimental observations, such as area/lipid, since surface tension is an essential factor in determining the monolayer structure. Data analysis on DLGPC/water monolayer shows that various liquid-condensed phase properties of the monolayer have been well reproduced from the simulation, indicating that surface tension 22.9mN/m used in the simulation is an appropriate condition for the condensed phase NPNgammaT simulation. The simulation results suggest that this long-chain phospholipid monolayer shares many structural characteristics with typical short-chain 1,2-diacylphosphatidylcholine systems. Furthermore, it was found that DLGPC/water monolayer has almost completely rotationally disordered acyl chains which have not been observed so far in short-chain 1,2-diacylphosphatidylcholine/water bilayers. The simulation indicates the good biological relevance of the DLGPC/water monolayer which might be useful in protein/lipid studies to reveal protein structure and protein-lipid interactions in a membrane environment.
520 # $a Vpu is an accessory membrane protein encoded by human immunodeficiency virus type 1 (HIV-1). Various experimental studies have shown that transmembrane domain of Vpu has a helical conformation and cytoplasmic domain adopts the helix-loop-helix-turn motif. This 3.5-ns molecular dynamics simulation of Vpu in lipid membrane environment has fully reproduced these structural characteristics. Membrane propensities of two amphipathic helices in cytoplasmic domain are further compared here to understand better their complicated orientational behavior known from the experiments. This study first reveals that the highly conserved loop region in cytoplasmic domain can be closely associated with membrane surface. It is known from the simulation that Vpu is associated with 34 lipids in this Langmuir monolayer. The lipids that are located between Vpu transmembrane helix and the first helix in cytoplasmic domain are pushed up by Vpu. These elevated lipids have increased P-N tilt angle for head groups yet with unchanged acyl-chain tilt angle compared with lipids that do not interact with Vpu. This study verifies the significance of applying MD simulation in refining protein structure and revealing detailed protein-lipid interaction in membrane/water environment.
590 $a School code: 0175.
650 # 0 $a Chemistry, Physical. $3 226924
650 # 0 $a Biophysics, Medical. $3 227040
650 # 0 $a Biology, Molecular. $3 226919
710 0 # $a University of Pennsylvania. $3 212781
773 0 # $g 64-04B. $t Dissertation Abstracts International
790 $a 0175
790 1 0 $a Licht, Walter, $e advisor
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://libsw.nuk.edu.tw/login?url=http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3087472 $z http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3087472