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Investigations and applications of model membrane systems

Record Type:	Electronic resources : Monograph/item
Title/Author:	Investigations and applications of model membrane systems
Author:	Johnson, Joseph Montgomerey.
Description:	134 p.
Notes:	Adviser: Steven G. Boxer.
Notes:	Source: Dissertation Abstracts International, Volume: 65-04, Section: B, page: 1846.
Contained By:	Dissertation Abstracts International65-04B.
Subject:	Chemistry, Biochemistry.
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3128655
ISBN:	0496759175

Investigations and applications of model membrane systems
Johnson, Joseph Montgomerey.

Investigations and applications of model membrane systems [electronic resource] - 134 p.

Adviser: Steven G. Boxer.

Thesis (Ph.D.)--Stanford University, 2004.

Control of two dimensional mixing in supported bilayer samples was achieved using an elastomeric wall. Bilayers of distinct composition could be formed on the same substrate, allowing mixing of the bilayers with a well defined start time. This could also be accomplished with vesicles of distinct composition. Vesicles were tethered through hybridization of oligo linkers covalently bonded to lipids. Vesicles were laterally fluid. Colocalization of vesicles was observed if they also contained complementary cross-linking oligos. Diffusion coefficients of colocalized vesicles were determined to be similar to that of isolated tethered vesicles.

ISBN: 0496759175Subjects--Topical Terms:

226900
Chemistry, Biochemistry.

Investigations and applications of model membrane systems
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245 1 0 $a Investigations and applications of model membrane systems $h [electronic resource]
300 $a 134 p.
500 $a Adviser: Steven G. Boxer.
500 $a Source: Dissertation Abstracts International, Volume: 65-04, Section: B, page: 1846.
502 $a Thesis (Ph.D.)--Stanford University, 2004.
520 # $a Control of two dimensional mixing in supported bilayer samples was achieved using an elastomeric wall. Bilayers of distinct composition could be formed on the same substrate, allowing mixing of the bilayers with a well defined start time. This could also be accomplished with vesicles of distinct composition. Vesicles were tethered through hybridization of oligo linkers covalently bonded to lipids. Vesicles were laterally fluid. Colocalization of vesicles was observed if they also contained complementary cross-linking oligos. Diffusion coefficients of colocalized vesicles were determined to be similar to that of isolated tethered vesicles.
520 # $a Formation of supported bilayers via vesicle fusion to silica substrates was investigated at a single vesicle level, using both fluorescence microscopy and atomic force microscopy (AFM). Different pathways for bilayer formation were observed, including primary vesicle fusion followed by rupture and simultaneous fusion and rupture. Approximately 50% of the vesicles observed underwent content loss dependent upon the presence of 6% fluorescently labeled lipid, Texas Red DHPE. AFM determined content loss to be due to leakage of adsorbed vesicles, as opposed to rupture of vesicles to form bilayer discs. From the flattening ratio of adsorbed vesicles (5:1) the adhesion potential was determined to be 0.1 mJ/m2 and the critical rupture radius of vesicles was estimated as >250 nm.
520 # $a Rheological properties of fluorescently labeled lambda DNA adsorbed to fluid, supported bilayers are studied. During electrophoresis DNA molecules translated in extended confirmations, due to interactions with obstacles on the bilayer surface. The obstacle density on the bilayer was estimated from the time scale of polymer extension/retraction cycles to be 0.03--0.05 mum -2. It was found that hydrodynamic drag on DNA molecules was increased by 80% when interacting with obstacles. DNA molecules were observed to undergo anomalous diffusion, with a large increase in the diffusion coefficient after electrophoresis.
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650 # 0 $a Chemistry, Biochemistry. $3 226900
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