語系:
繁體中文
English
說明(常見問題)
圖資館首頁
登入
回首頁
切換:
標籤
|
MARC模式
|
ISBD
Synaptic vesicle cycling: Mechanisms...
~
Aravanis, Alexander M.
Synaptic vesicle cycling: Mechanisms and implications for information transfer across and synapse.
紀錄類型:
書目-電子資源 : Monograph/item
正題名/作者:
Synaptic vesicle cycling: Mechanisms and implications for information transfer across and synapse.
作者:
Aravanis, Alexander M.
面頁冊數:
174 p.
附註:
Adviser: Richard Tsien.
附註:
Source: Dissertation Abstracts International, Volume: 66-08, Section: B, page: 4383.
Contained By:
Dissertation Abstracts International66-08B.
標題:
Engineering, Electronics and Electrical.
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3187261
ISBN:
9780542294600
Synaptic vesicle cycling: Mechanisms and implications for information transfer across and synapse.
Aravanis, Alexander M.
Synaptic vesicle cycling: Mechanisms and implications for information transfer across and synapse.
- 174 p.
Adviser: Richard Tsien.
Thesis (Ph.D.)--Stanford University, 2005.
At synapses of the mammalian central nervous system, release of neurotransmitter occurs at rates transiently as high as 100 Hz, putting extreme demands on the tiny nerve terminals of the brain, typically containing ∼30 functional vesicles. However, the modes of exocytosis and endocytosis that operate at CNS synapses are incompletely understood. Work in this thesis provided the first real-time visualization of a single vesicle fusing at a small central synapse, made possible by highly-intensified CCD imaging of hippocampal synaptic terminals, in which a single vesicle was labelled with the fluorescent membrane marker FM1-43. In a minority of cases, full loss of FM dye was elicited by a single action potential, consistent with classical complete collapse. In most cases, however, action potentials triggered only partial loss of fluorescence, suggesting vesicular retention of membrane marker, consistent with "kiss-and-run" vesicle cycling. An alternative hypothesis, the independent fusion of partially stained vesicles arising from endosomal splitting, could be excluded by observations on the size and timing of successive fusion events. Thus, these experimental evidence support a predominance of kiss-and-run fusion events and rapid vesicular reuse. The results of the single vesicle experiments are most relevant to the readily-releasable pool of vesicles, a subset of the total recycling pool, because of the minimal stimulation protocol used. The properties of the entire vesicle pool were also studied, using novel fluorescent quenching strategies and linear systems theory. They demonstrated that the whole vesicle pool is capable of undergoing kiss-and-run, and that the amount of kiss-and-run on a per fusion basis was greater at low frequencies. Finally, a simple model of the vesicle cycle was used to determine the impact of kiss-and-run on the transfer of information across the synapse. The results indicated that kiss-and-run significantly increased both overall and burst transmission.
ISBN: 9780542294600Subjects--Topical Terms:
226981
Engineering, Electronics and Electrical.
Synaptic vesicle cycling: Mechanisms and implications for information transfer across and synapse.
LDR
:02969nmm _2200253 _450
001
170834
005
20061228142257.5
008
090528s2005 eng d
020
$a
9780542294600
035
$a
00242864
040
$a
UnM
$c
UnM
100
0
$a
Aravanis, Alexander M.
$3
244865
245
1 0
$a
Synaptic vesicle cycling: Mechanisms and implications for information transfer across and synapse.
300
$a
174 p.
500
$a
Adviser: Richard Tsien.
500
$a
Source: Dissertation Abstracts International, Volume: 66-08, Section: B, page: 4383.
502
$a
Thesis (Ph.D.)--Stanford University, 2005.
520
#
$a
At synapses of the mammalian central nervous system, release of neurotransmitter occurs at rates transiently as high as 100 Hz, putting extreme demands on the tiny nerve terminals of the brain, typically containing ∼30 functional vesicles. However, the modes of exocytosis and endocytosis that operate at CNS synapses are incompletely understood. Work in this thesis provided the first real-time visualization of a single vesicle fusing at a small central synapse, made possible by highly-intensified CCD imaging of hippocampal synaptic terminals, in which a single vesicle was labelled with the fluorescent membrane marker FM1-43. In a minority of cases, full loss of FM dye was elicited by a single action potential, consistent with classical complete collapse. In most cases, however, action potentials triggered only partial loss of fluorescence, suggesting vesicular retention of membrane marker, consistent with "kiss-and-run" vesicle cycling. An alternative hypothesis, the independent fusion of partially stained vesicles arising from endosomal splitting, could be excluded by observations on the size and timing of successive fusion events. Thus, these experimental evidence support a predominance of kiss-and-run fusion events and rapid vesicular reuse. The results of the single vesicle experiments are most relevant to the readily-releasable pool of vesicles, a subset of the total recycling pool, because of the minimal stimulation protocol used. The properties of the entire vesicle pool were also studied, using novel fluorescent quenching strategies and linear systems theory. They demonstrated that the whole vesicle pool is capable of undergoing kiss-and-run, and that the amount of kiss-and-run on a per fusion basis was greater at low frequencies. Finally, a simple model of the vesicle cycle was used to determine the impact of kiss-and-run on the transfer of information across the synapse. The results indicated that kiss-and-run significantly increased both overall and burst transmission.
590
$a
School code: 0212.
650
# 0
$a
Engineering, Electronics and Electrical.
$3
226981
690
$a
0544
710
0 #
$a
Stanford University.
$3
212607
773
0 #
$g
66-08B.
$t
Dissertation Abstracts International
790
$a
0212
790
1 0
$a
Tsien, Richard,
$e
advisor
791
$a
Ph.D.
792
$a
2005
856
4 0
$u
http://libsw.nuk.edu.tw:81/login?url=http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3187261
$z
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3187261
筆 0 讀者評論
全部
電子館藏
館藏
1 筆 • 頁數 1 •
1
條碼號
館藏地
館藏流通類別
資料類型
索書號
使用類型
借閱狀態
預約狀態
備註欄
附件
000000002632
電子館藏
1圖書
學位論文
一般使用(Normal)
在架
0
1 筆 • 頁數 1 •
1
多媒體
多媒體檔案
http://libsw.nuk.edu.tw:81/login?url=http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3187261
評論
新增評論
分享你的心得
Export
取書館別
處理中
...
變更密碼
登入