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製備聚乙烯乙烯醇/量子點複合奈米微粒之分子模版應用於分子感測及其性質分析...

何旻賢

製備聚乙烯乙烯醇/量子點複合奈米微粒之分子模版應用於分子感測及其性質分析 = Synthesis, Characterization and Sensing Applications ofMolecularly Imprinted Poly(ethylene-co-vinyl alcohol)/Quantum Dots Composite Nanoparticles

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Synthesis, Characterization and Sensing Applications ofMolecularly Imprinted Poly(ethylene-co-vinyl alcohol)/Quantum Dots Composite Nanoparticles
作者:	何旻賢,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	2009[民98]
面頁冊數:	137面圖、表 : 30公分;
標題:	分子印模
標題:	albumin
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/00475011325988022636
附註:	指導教授：林宏殷
附註:	參考書目：面
摘要註:	量子點的螢光穩定性常被應用於生物學上做為標示使用，而取代了傳統的螢光染劑。抗體包覆量子點外層去標定抗原的方式與使用分子拓印技術製備類似抗體位子去吸附目標分子的方式相似，因此，利用分子拓印高分子包覆量子點亦可具有模版孔洞與選擇性，本研究以聚乙烯-乙烯醇（poly(ethylene-co-vinyl alcohol), EVAL）混合不同放射波長的量子點加入沉澱槽，利用相轉換的方式進行快速製備分子拓印高分子複合奈米微粒，進行型態、再吸附性質分析，並藉由量子點的螢光穩定性變化檢測尿液中肌酸酐、白蛋白、溶菌酶分子濃度，與醫院生化檢測濃度做準確率之比較。 The high stability of quantum dots (QDots) with photoluminescence has led to their increase use as imaging approaches in biological systems to replace conventional fluorescence labels. The antibodies are generally coated on the surface of QDots to the targeting site, and molecular imprinting polymers are designed to mimic the antibodies. Hence, quantum dots can be incorporated into molecularly imprinted polymers, which provide shape and selectivity, and then respond to template rebinding by emitting quenched photoluminescence. In this study, poly(ethylene-co-ethylene alcohol) creatinine-, albumin- and lysozyme-imprinted polymers nanoparticles are synthesized via phase inversion of poly(ethylene-co-ethylene alcohol) with various ethylene mole ratios when target molecules and hydrophobic quantum dots are mixed within the polymer solution. Finally, those particles were prepared for the detection of creatinine, human serum albumin and lysozyme in real sample (urine) and compared with commercial ARCHITECT ci 8200 system.Keywords: poly(ethylene-co-ethylene alcohol); creatinine; albumin; lysozyme; molecular imprinting; urine.

製備聚乙烯乙烯醇/量子點複合奈米微粒之分子模版應用於分子感測及其性質分析 = Synthesis, Characterization and Sensing Applications ofMolecularly Imprinted Poly(ethylene-co-vinyl alcohol)/Quantum Dots Composite Nanoparticles
何, 旻賢

製備聚乙烯乙烯醇/量子點複合奈米微粒之分子模版應用於分子感測及其性質分析 = Synthesis, Characterization and Sensing Applications ofMolecularly Imprinted Poly(ethylene-co-vinyl alcohol)/Quantum Dots Composite Nanoparticles / 何旻賢撰 - [高雄市] : 撰者, 2009[民98]. - 137面 ; 圖、表 ; 30公分.
指導教授：林宏殷參考書目：面.
分子印模albumin

製備聚乙烯乙烯醇/量子點複合奈米微粒之分子模版應用於分子感測及其性質分析 = Synthesis, Characterization and Sensing Applications ofMolecularly Imprinted Poly(ethylene-co-vinyl alcohol)/Quantum Dots Composite Nanoparticles
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330 $a 量子點的螢光穩定性常被應用於生物學上做為標示使用，而取代了傳統的螢光染劑。抗體包覆量子點外層去標定抗原的方式與使用分子拓印技術製備類似抗體位子去吸附目標分子的方式相似，因此，利用分子拓印高分子包覆量子點亦可具有模版孔洞與選擇性，本研究以聚乙烯-乙烯醇（poly(ethylene-co-vinyl alcohol), EVAL）混合不同放射波長的量子點加入沉澱槽，利用相轉換的方式進行快速製備分子拓印高分子複合奈米微粒，進行型態、再吸附性質分析，並藉由量子點的螢光穩定性變化檢測尿液中肌酸酐、白蛋白、溶菌酶分子濃度，與醫院生化檢測濃度做準確率之比較。 The high stability of quantum dots (QDots) with photoluminescence has led to their increase use as imaging approaches in biological systems to replace conventional fluorescence labels. The antibodies are generally coated on the surface of QDots to the targeting site, and molecular imprinting polymers are designed to mimic the antibodies. Hence, quantum dots can be incorporated into molecularly imprinted polymers, which provide shape and selectivity, and then respond to template rebinding by emitting quenched photoluminescence. In this study, poly(ethylene-co-ethylene alcohol) creatinine-, albumin- and lysozyme-imprinted polymers nanoparticles are synthesized via phase inversion of poly(ethylene-co-ethylene alcohol) with various ethylene mole ratios when target molecules and hydrophobic quantum dots are mixed within the polymer solution. Finally, those particles were prepared for the detection of creatinine, human serum albumin and lysozyme in real sample (urine) and compared with commercial ARCHITECT ci 8200 system.Keywords: poly(ethylene-co-ethylene alcohol); creatinine; albumin; lysozyme; molecular imprinting; urine.
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