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Design and application of composite ...

State University of New York at Albany.

Design and application of composite RNA aptamers.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Design and application of composite RNA aptamers.
Author:	Wang, Shengchun.
Description:	127 p.
Notes:	Source: Dissertation Abstracts International, Volume: 72-11, Section: B, page: .
Notes:	Adviser: Hua Shi.
Contained By:	Dissertation Abstracts International72-11B.
Subject:	Biology, Molecular.
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3466784
ISBN:	9781124804927

Design and application of composite RNA aptamers.
Wang, Shengchun.

Design and application of composite RNA aptamers. - 127 p.

Source: Dissertation Abstracts International, Volume: 72-11, Section: B, page: .

Thesis (Ph.D.)--State University of New York at Albany, 2011.

RNA aptamers are being developed as an essential tool in many fields of biological research. Their utility is not limited to being protein inhibitors; a lot of novel functions can be realized. However, in vivo application of RNA aptamers still faces many challenges. The aim of this dissertation is to design and apply composite aptamers in multiple expression and delivery systems to address some critical issues, such as correct folding, high level production, degradation by nucleases, excessive consumption of cellular resource and potential toxic effect.

ISBN: 9781124804927Subjects--Topical Terms:

226919
Biology, Molecular.

Design and application of composite RNA aptamers.
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245 1 0 $a Design and application of composite RNA aptamers.
300 $a 127 p.
500 $a Source: Dissertation Abstracts International, Volume: 72-11, Section: B, page: .
500 $a Adviser: Hua Shi.
502 $a Thesis (Ph.D.)--State University of New York at Albany, 2011.
520 $a RNA aptamers are being developed as an essential tool in many fields of biological research. Their utility is not limited to being protein inhibitors; a lot of novel functions can be realized. However, in vivo application of RNA aptamers still faces many challenges. The aim of this dissertation is to design and apply composite aptamers in multiple expression and delivery systems to address some critical issues, such as correct folding, high level production, degradation by nucleases, excessive consumption of cellular resource and potential toxic effect.
520 $a In one project, I used an intron homing process to mediate aptamer incorporation into the yeast rDNA, resulting in large amount accumulation of a HSF targeting aptamer without affecting normal cellular behavior. The aptamer expressed in this manner inhibited yeast heat shock response. In another project, I used a forward engineering approach to develop a multivalent RNA aptamer to assist the formation of transcription pre-initiation complex at promoter, making it an RNA based transcription activator (taRNA).
520 $a The design and implementation of these methods have demonstrated the potential to be adopted and modified for more precise gene regulation and have expanded the repertoire of tools in synthetic biology research using aptamer and other nucleic acid based materials.
590 $a School code: 0668.
650 4 $a Biology, Molecular. $3 226919
650 4 $a Biology, Cell. $3 226967
650 4 $a Chemistry, Biochemistry. $3 226900
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790 1 0 $a Lee, Min-Ho $e committee member
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