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Characterization of sequence variati...

Chandra, Yohanna Gita.

Characterization of sequence variation in viral genomes of wild type and vaccine strains of infectious laryngotracheitis virus (ILTV) using next generation sequencing.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Characterization of sequence variation in viral genomes of wild type and vaccine strains of infectious laryngotracheitis virus (ILTV) using next generation sequencing.
Author:	Chandra, Yohanna Gita.
Description:	106 p.
Notes:	Source: Masters Abstracts International, Volume: 49-05, page: 3059.
Notes:	Adviser: Byung-Whi Kong.
Contained By:	Masters Abstracts International49-05.
Subject:	Biology, Molecular.
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1493269
ISBN:	9781124640778

Characterization of sequence variation in viral genomes of wild type and vaccine strains of infectious laryngotracheitis virus (ILTV) using next generation sequencing.
Chandra, Yohanna Gita.

Characterization of sequence variation in viral genomes of wild type and vaccine strains of infectious laryngotracheitis virus (ILTV) using next generation sequencing. - 106 p.

Source: Masters Abstracts International, Volume: 49-05, page: 3059.

Thesis (M.S.)--University of Arkansas, 2011.

The study was conducted to identify the unique variations in wild type and vaccine strain of ILTV genomes to obtain complete mutations within the entire genomes compared to a reference ILTV genome sequence ( Gallid herpesvirus 1, complete genome; GenBank accession number NC_006623). Genomes of one wild type and two vaccine ILTV strains were sequenced using Illumina Genome Analyzer 2X of 36 cycles of single-end read. Analysis of the ILTV genome sequences was carried out using a trial version of DNAStar software (including NGen, SeqMan Pro, Gen Vision), Blastx and Microsoft Excel. Results showed many amino acid differences distributed across the viral protein coding regions of the three ILTV genomes compared to the reference genome sequence. The sequence differences demonstrated the spectrum of variability among the wild type and vaccine strains. Moreover, the results also revealed 52 genes with complete conservation across the 3 strains. These data demonstrated the potential of high-throughput sequencing technology to yield insight of the sequence variation in different ILTV strains. This information can be used to discriminate between wild type and vaccine strains, or among vaccines strains, and further, to identify newly emerging mutant strains. The conserved coding sequences among various ILTV strains can be promising targets to discover a universal, safer vaccine against ILTV.

ISBN: 9781124640778Subjects--Topical Terms:

226919
Biology, Molecular.

Characterization of sequence variation in viral genomes of wild type and vaccine strains of infectious laryngotracheitis virus (ILTV) using next generation sequencing.
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100 1 $a Chandra, Yohanna Gita. $3 542086
245 1 0 $a Characterization of sequence variation in viral genomes of wild type and vaccine strains of infectious laryngotracheitis virus (ILTV) using next generation sequencing.
300 $a 106 p.
500 $a Source: Masters Abstracts International, Volume: 49-05, page: 3059.
500 $a Adviser: Byung-Whi Kong.
502 $a Thesis (M.S.)--University of Arkansas, 2011.
520 $a The study was conducted to identify the unique variations in wild type and vaccine strain of ILTV genomes to obtain complete mutations within the entire genomes compared to a reference ILTV genome sequence ( Gallid herpesvirus 1, complete genome; GenBank accession number NC_006623). Genomes of one wild type and two vaccine ILTV strains were sequenced using Illumina Genome Analyzer 2X of 36 cycles of single-end read. Analysis of the ILTV genome sequences was carried out using a trial version of DNAStar software (including NGen, SeqMan Pro, Gen Vision), Blastx and Microsoft Excel. Results showed many amino acid differences distributed across the viral protein coding regions of the three ILTV genomes compared to the reference genome sequence. The sequence differences demonstrated the spectrum of variability among the wild type and vaccine strains. Moreover, the results also revealed 52 genes with complete conservation across the 3 strains. These data demonstrated the potential of high-throughput sequencing technology to yield insight of the sequence variation in different ILTV strains. This information can be used to discriminate between wild type and vaccine strains, or among vaccines strains, and further, to identify newly emerging mutant strains. The conserved coding sequences among various ILTV strains can be promising targets to discover a universal, safer vaccine against ILTV.
520 $a KEYWORDS: ILTV genome, Illumina sequencing, variation.
590 $a School code: 0011.
650 4 $a Biology, Molecular. $3 226919
650 4 $a Biology, Bioinformatics. $3 264207
650 4 $a Biology, Virology. $3 264226
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710 2 $a University of Arkansas. $3 542087
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