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以鹼性磷酸酶拓印聚乙烯乙烯醇/量子點複合微粒檢測及標識誘導型多潛能性幹細...

國立高雄大學化學工程及材料工程學系碩士班

以鹼性磷酸酶拓印聚乙烯乙烯醇/量子點複合微粒檢測及標識誘導型多潛能性幹細胞 = Sensing and labeling of induced pluripotent stem cell by alkaline phosphatase-imprinted poly(ethylene-co-vinyl alcohol)/quantum dots composite nanoparticles

Record Type:	Language materials, printed : monographic
Paralel Title:	Sensing and labeling of induced pluripotent stem cell by alkaline phosphatase-imprinted poly(ethylene-co-vinyl alcohol)/quantum dots composite nanoparticles
Author:	洪子麟,
Secondary Intellectual Responsibility:	國立高雄大學
Place of Publication:	[高雄市]
Published:	撰者;
Year of Publication:	2012[民101]
Description:	122面圖，表格 : 30公分;
Subject:	誘導型多潛能性幹細胞
Subject:	induced pluripotent stem cells
Online resource:	http://ethesys.nuk.edu.tw/ETD-db/ETD-search-c/view_etd?URN=etd-0819112-193355
Notes:	106年10月31日公開
Notes:	參考書目：面100-105
[NT 15001349]:	以鹼性磷酸酶拓印聚乙烯乙烯醇量子點複合微粒檢測及標識誘導型多潛能性幹細胞
Summary:	本研究利用慢病毒載體將誘導型多潛能性幹細胞轉錄因子(Oct4、Sox2、C-myc、Klf4)送入人類胚胎腎臟細胞(HEK293 cells)進行基因重組，持續培養成為誘導型多潛能性幹細胞株(iPS cells)，透過觀察誘導型多潛能性幹細胞其細胞型態，並利用多種鑑定方式如洋菜膠DNA電泳法、即時螢光核酸定量法、免疫螢光染色法及拉曼圖譜分析鑑定iPS細胞其基因表現，由於幹細胞的生長與鹼性磷酸酶的含量有密切的關係，因此利用分子拓印磁性/光學奈米複合微粒進行iPS細胞中的鹼性磷酸酶標示及其細胞液內的含量分析，與醫院生化檢測濃度做準確率之比較。 In this study, by using lentiviral vector with four transcription factors (Oct4、Sox2、C-myc、Klf4) reprogrammed Human Embryonic Kidney cells (HEK293 cells) to induced pluripotent stem cells (iPS cells). With cell morphology observation and variety of methods to identify its gene expression, such as agarose gel DNA electrophoresis, real-time polymerase chain reaction, immunofluorescence and Raman spectroscopy analysis. In the cell growth procedure, alkaline phosphatase concentration is closely related. Therefore, alkaline phosphatase-imprinted poly(ethylene-co-vinyl alcohol)/quantum dots composite nanoparticles can be labeling and sensing those molecules in the cells. Finally, nanoparticles were prepared for the detection of alkaline phosphatase in cell medium and compared with commercial ARCHITECT ci 8200 system.

以鹼性磷酸酶拓印聚乙烯乙烯醇/量子點複合微粒檢測及標識誘導型多潛能性幹細胞 = Sensing and labeling of induced pluripotent stem cell by alkaline phosphatase-imprinted poly(ethylene-co-vinyl alcohol)/quantum dots composite nanoparticles
洪, 子麟

以鹼性磷酸酶拓印聚乙烯乙烯醇/量子點複合微粒檢測及標識誘導型多潛能性幹細胞 = Sensing and labeling of induced pluripotent stem cell by alkaline phosphatase-imprinted poly(ethylene-co-vinyl alcohol)/quantum dots composite nanoparticles / 洪子麟撰 - [高雄市] : 撰者, 2012[民101]. - 122面 ; 圖，表格 ; 30公分.
106年10月31日公開參考書目：面100-105.
誘導型多潛能性幹細胞induced pluripotent stem cells

以鹼性磷酸酶拓印聚乙烯乙烯醇/量子點複合微粒檢測及標識誘導型多潛能性幹細胞 = Sensing and labeling of induced pluripotent stem cell by alkaline phosphatase-imprinted poly(ethylene-co-vinyl alcohol)/quantum dots composite nanoparticles
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