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製備聚乙烯-乙烯醇/磁性複合微粒之分子模版應用於澱粉酶催化 = Synt...

國立高雄大學化學工程及材料工程學系碩士班

製備聚乙烯-乙烯醇/磁性複合微粒之分子模版應用於澱粉酶催化 = Synthesis of Amylase-imprinted poly(ethylene-co-vinyl alcohol) magnetic composited nanoparticles for the applications of hydrolysis

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Synthesis of Amylase-imprinted poly(ethylene-co-vinyl alcohol) magnetic composited nanoparticles for the applications of hydrolysis
作者:	陳詠超,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	2011[民100]
面頁冊數:	81面圖，表格 : 30公分;
標題:	聚乙烯-乙烯醇
標題:	Molecularly imprinting
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/33218345052304062334
附註:	參考書目：面75-77
摘要註:	MIPs近年來時常用於辨識元素的應用上，或是具有良好的控制性的對小分毒害物質的傳輸，將MIPs 用於活性酵素固定或束縛裡，在研究上是缺乏且重要，因此在本次的研究中辨識酵素Amylase拓印磁性MIPs是將Amylase充分溶解於不同乙烯莫耳比的聚乙烯-乙烯醇中，且經由相轉換而成。藉由DLS、BET、SQUID、XRD可測得粒徑分佈、比表面積、磁化量、晶體結構組成，所以可知固定化模版的特性，然而固定化酵素活性是藉由對於不同成分比例的MIP（有重量百分比、或莫耳比），且在不同溫度下來量測澱粉水解葡萄糖產物。澱粉酶拓印聚乙烯-乙烯醇磁性複合微粒在600C下澱粉水解可以重複60次。 Molecularly imprinted polymers (MIPs) have frequently been employed as recognition elements in sensing applications, or for the controlled delivery of small molecule drugs. An equally important but less well stuied application is the use of MIPs in the binding and immobilization of active enzymes. In this study, magnetic MIPs (MMIPs) recognizing the enzyme amylase were prepared using phase inversion of poly(ethylene-co-vinyl alcohol) (EVAL) solutions with various ethylene mole ratios in the presence of amylase. The size distribution,specific surface area, magnetization, and composition were characterized by dynamic light scattering, Brunauer-Emmett-Teller (BET) analysis, superconducting quantum interference devices, and X-ray diffraction, respectively. The activities of both bound template and rebound enzyme was established by measuring glucose production via starch hydrolysis,at different temperature, for MIPs with different compositions (weight % of EVALs and mole % of ethylene ).Finally, the reusability of magnetic amylase-imprinted EVAL nanoparticles was examined and starch hydrolysis for 60 times at 60oC.

製備聚乙烯-乙烯醇/磁性複合微粒之分子模版應用於澱粉酶催化 = Synthesis of Amylase-imprinted poly(ethylene-co-vinyl alcohol) magnetic composited nanoparticles for the applications of hydrolysis
陳, 詠超

製備聚乙烯-乙烯醇/磁性複合微粒之分子模版應用於澱粉酶催化 = Synthesis of Amylase-imprinted poly(ethylene-co-vinyl alcohol) magnetic composited nanoparticles for the applications of hydrolysis / 陳詠超撰 - [高雄市] : 撰者, 2011[民100]. - 81面 ; 圖，表格 ; 30公分.
參考書目：面75-77.
聚乙烯-乙烯醇Molecularly imprinting

製備聚乙烯-乙烯醇/磁性複合微粒之分子模版應用於澱粉酶催化 = Synthesis of Amylase-imprinted poly(ethylene-co-vinyl alcohol) magnetic composited nanoparticles for the applications of hydrolysis
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330 $a MIPs近年來時常用於辨識元素的應用上，或是具有良好的控制性的對小分毒害物質的傳輸，將MIPs 用於活性酵素固定或束縛裡，在研究上是缺乏且重要，因此在本次的研究中辨識酵素Amylase拓印磁性MIPs是將Amylase充分溶解於不同乙烯莫耳比的聚乙烯-乙烯醇中，且經由相轉換而成。藉由DLS、BET、SQUID、XRD可測得粒徑分佈、比表面積、磁化量、晶體結構組成，所以可知固定化模版的特性，然而固定化酵素活性是藉由對於不同成分比例的MIP（有重量百分比、或莫耳比），且在不同溫度下來量測澱粉水解葡萄糖產物。澱粉酶拓印聚乙烯-乙烯醇磁性複合微粒在600C下澱粉水解可以重複60次。 Molecularly imprinted polymers (MIPs) have frequently been employed as recognition elements in sensing applications, or for the controlled delivery of small molecule drugs. An equally important but less well stuied application is the use of MIPs in the binding and immobilization of active enzymes. In this study, magnetic MIPs (MMIPs) recognizing the enzyme amylase were prepared using phase inversion of poly(ethylene-co-vinyl alcohol) (EVAL) solutions with various ethylene mole ratios in the presence of amylase. The size distribution,specific surface area, magnetization, and composition were characterized by dynamic light scattering, Brunauer-Emmett-Teller (BET) analysis, superconducting quantum interference devices, and X-ray diffraction, respectively. The activities of both bound template and rebound enzyme was established by measuring glucose production via starch hydrolysis,at different temperature, for MIPs with different compositions (weight % of EVALs and mole % of ethylene ).Finally, the reusability of magnetic amylase-imprinted EVAL nanoparticles was examined and starch hydrolysis for 60 times at 60oC.
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