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鹼基拓印甲殼素磁性複合奈米粒子的製備及其在基因轉染及細胞凋亡的應用 = ...

國立高雄大學化學工程及材料工程學系碩士班

鹼基拓印甲殼素磁性複合奈米粒子的製備及其在基因轉染及細胞凋亡的應用 = Preparation and Characterization of Magnetic NucleobasesImprinted Chitosan Composite Nanoparticles and TheirApplications in Gene Transfection and Cellular Apoptosis

Record Type:	Language materials, printed : monographic
Paralel Title:	Preparation and Characterization of Magnetic NucleobasesImprinted Chitosan Composite Nanoparticles and TheirApplications in Gene Transfection and Cellular Apoptosis
Author:	陳建州,
Secondary Intellectual Responsibility:	國立高雄大學
Place of Publication:	[高雄市]
Published:	撰者;
Year of Publication:	2014[民103]
Description:	115面圖，表 : 30公分;
Subject:	磁性基因載體
Subject:	Magnetic Gene Vectors
Online resource:	http://handle.ncl.edu.tw/11296/ndltd/10241944947949106736
Notes:	參考書目：面86-94
Notes:	108年10月31日公開
Summary:	本研究結合分子拓印技術、甲殼素及磁性奈米粒子，製備鹼基分子拓印甲殼素磁性奈米粒子作為基因載體，並應用於轉染及誘導癌細胞凋亡。以相變化的方式在水相進行製備，保留材料原始特性並維持系統單純，減少成本、耗時同時避免額外的細胞毒性。將0.1g/L鹼基分子拓印於0.01wt%、分子量370k之甲殼素製備複合奈米粒子，利用高效液相層析儀(HPLC)、穿透式電子顯微鏡(TEM)、粒徑分析儀及拉曼光譜儀等進行複合粒子性質分析後，對HEK293及HepG2細胞進行給藥，發現複合甲殼素後可有效減少磁性粉體約35~50%的細胞毒性，利用胞嘧啶拓印甲殼素複合粒子(Cytosine Imprinted Polymers, CIPs)吸附綠螢光蛋白基因，同時外加磁場對HEK293細胞進行轉染，效率可達市售載體的五成以上；另外以胸腺嘧啶拓印甲殼素複合粒子(Thymine Imprinted Polymers, TIPs)阻礙端粒體行為，結果在給藥後發現癌細胞分裂明顯趨緩，若在表面吸附短片段人工端可成功誘導癌細胞凋亡。 This study combines molecular imprinting technology, chitosan and magnetic nanoparticles to prepare magnetic nucleobases imprinted chitosan composite nanoparticles (MNIC-NPs) as gene vectors. During the experiments, we imprint adenine, cytosine and thymine on chitosan and use them for gene transfection and induction of cancer cells apoptosis. Using phase transformation to prepare MNIC-NPs in aqueous solution can maintain the nature properties of chitosan and simplify the system, which also can decrease cost and prevent excess cytotoxicity. Imprinting 0.1g/L nucleobases on 0.01wt% chitosan with 370k M.W., then analyzing properties by high performance liquid chromatography, transmission electron microscopy, particle size analyzer and Raman spectrum. Chitosan can reduce 35~50% cytotoxicity of magnetic particles which dose to HEK293 and HepG2 cells. Adsorb green fluorescent protein gene (pEGFP) with cytosine imprinted polymers (CIPs) and transfect into HEK293 cells by magnetic field, the efficiency can surpass 50% which compare with commercial vectors (Lipofection 2000). After dosing thymine imprinted polymers (TIPs, which are expected to block the function of telomeres) into HepG2 cells, the growth rate slowing down obviously. Furthermore, rebinding artificially short telomeres on TIPs before dosing can successfully induce apoptosis of HepG2.

鹼基拓印甲殼素磁性複合奈米粒子的製備及其在基因轉染及細胞凋亡的應用 = Preparation and Characterization of Magnetic NucleobasesImprinted Chitosan Composite Nanoparticles and TheirApplications in Gene Transfection and Cellular Apoptosis
陳, 建州

鹼基拓印甲殼素磁性複合奈米粒子的製備及其在基因轉染及細胞凋亡的應用 = Preparation and Characterization of Magnetic NucleobasesImprinted Chitosan Composite Nanoparticles and TheirApplications in Gene Transfection and Cellular Apoptosis / 陳建州撰 - [高雄市] : 撰者, 2014[民103]. - 115面 ; 圖，表 ; 30公分.
參考書目：面86-94108年10月31日公開.
磁性基因載體Magnetic Gene Vectors

鹼基拓印甲殼素磁性複合奈米粒子的製備及其在基因轉染及細胞凋亡的應用 = Preparation and Characterization of Magnetic NucleobasesImprinted Chitosan Composite Nanoparticles and TheirApplications in Gene Transfection and Cellular Apoptosis
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