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以水熱法控制氧化鋅粒子形貌並探討其抑菌及細胞凋亡路徑 = Morphol...
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國立高雄大學化學工程及材料工程學系碩士班
以水熱法控制氧化鋅粒子形貌並探討其抑菌及細胞凋亡路徑 = Morphology Control of Zinc Oxide Particles via Hydrothermal Route and Their Antibacterial Activity and Cellular Apoptosis
紀錄類型:
書目-語言資料,印刷品 : 單行本
並列題名:
Morphology Control of Zinc Oxide Particles via Hydrothermal Route and Their Antibacterial Activity and Cellular Apoptosis
作者:
紀明宏,
其他團體作者:
國立高雄大學
出版地:
[高雄市]
出版者:
撰者;
出版年:
2014[民103]
面頁冊數:
115面圖,表 : 30公分;
標題:
氧化鋅微粒
標題:
Zinc oxide
附註:
參考書目:面100-104
附註:
108年10月31日公開
摘要註:
近年來氧化鋅微粒已被廣泛製造,並應用於日常生活當中,如化妝品等。本研究以水熱合成法製備氧化鋅微粒,利用控制還原鹼液種類、酸鹼度及反應攪動等參數來控制氧化鋅微粒之形貌,並以電子顯微鏡(SEM)、X光繞射儀(XRD)、螢光分光光度計(PL)、比表面積分析儀(BET)分別分析氧化鋅微粒之形貌、晶型、光學特性、比表面積及在液體懸浮性質等,最後測試其對新生兒包皮細胞(Hs68)之細胞毒性測試,並以肉湯稀釋法對大腸桿菌(E. Coli)進行抑菌測試。 合成出之氧化鋅微粒形貌可分為鉛筆狀、花朵狀、球狀、短六角柱狀,發現氧化鋅以纖鋅礦(Wurzite)晶型結晶且短六角狀結晶性最高,而不同形貌螢光散射波長落在467nm,其中球型氧化鋅在液體懸浮性為最佳,以球型氧化鋅在細胞毒性測試及抗菌實驗,Hs68細胞能忍受球形氧化鋅微粒之最高及大腸桿菌最小抑菌濃度分別為1.6x102 In this study, the zinc oxide products have been synthesized by using a hydrothermal method. Moreover, the size and morphology of as-prepared ZnO particles differed much from each other and were found be strongly dependent on the pH values of solutions, species and concentration of precipitation agent, and agitation. The zinc oxide products having different morphologies ( lower-like, sphere, pencil-like, shrt hexagonal cylinder) were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), photoluminescence (PL) spectroscopy, Brunauer–Emmett–Teller (BET). In addition, the antibacterial activity and cytotoxicity of wurtzite zinc oxide products were investigated. It is found that the spherical ZnO nanoparticles exhibited the lowest cytotoxicity to human foreskin fibroblast (Hs68) when the ZnO concentration was lower than of 1.6x102μg/ml, and the minimum inhibitory concentration (MIC) in antibacterial activity of Escherichia. Coli (E.Coli) was 3.2x102μg/ml using broth dilution method .Adding spherical ZnO nanoparticles in commercial polymer solution to form liquid barrier film, which liquid barrier film dries quickly to form a breathable, transparent coating on the surface of everything in the air. The effect of ZnO nanoparticles in polymer solution were investigated under ultraviolet A (UV-A) irradiation. The results indicated that Hs68 cell growth index with ZnO nanoparticles was 1.4 times than without ZnO as irradiated time for 30min. Finally, we found that the apoptotic pathway in Hs68 cell using apoptotic genes (TP53, Bax, Bcl-2, Caspase-3). The result indicates the ZnO adding in polymer solution can be protect Hs 68 cell and then reduce cell apoptosis under UV-A irradiation
以水熱法控制氧化鋅粒子形貌並探討其抑菌及細胞凋亡路徑 = Morphology Control of Zinc Oxide Particles via Hydrothermal Route and Their Antibacterial Activity and Cellular Apoptosis
紀, 明宏
以水熱法控制氧化鋅粒子形貌並探討其抑菌及細胞凋亡路徑
= Morphology Control of Zinc Oxide Particles via Hydrothermal Route and Their Antibacterial Activity and Cellular Apoptosis / 紀明宏撰 - [高雄市] : 撰者, 2014[民103]. - 115面 ; 圖,表 ; 30公分.
參考書目:面100-104108年10月31日公開.
氧化鋅微粒Zinc oxide
以水熱法控制氧化鋅粒子形貌並探討其抑菌及細胞凋亡路徑 = Morphology Control of Zinc Oxide Particles via Hydrothermal Route and Their Antibacterial Activity and Cellular Apoptosis
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近年來氧化鋅微粒已被廣泛製造,並應用於日常生活當中,如化妝品等。本研究以水熱合成法製備氧化鋅微粒,利用控制還原鹼液種類、酸鹼度及反應攪動等參數來控制氧化鋅微粒之形貌,並以電子顯微鏡(SEM)、X光繞射儀(XRD)、螢光分光光度計(PL)、比表面積分析儀(BET)分別分析氧化鋅微粒之形貌、晶型、光學特性、比表面積及在液體懸浮性質等,最後測試其對新生兒包皮細胞(Hs68)之細胞毒性測試,並以肉湯稀釋法對大腸桿菌(E. Coli)進行抑菌測試。 合成出之氧化鋅微粒形貌可分為鉛筆狀、花朵狀、球狀、短六角柱狀,發現氧化鋅以纖鋅礦(Wurzite)晶型結晶且短六角狀結晶性最高,而不同形貌螢光散射波長落在467nm,其中球型氧化鋅在液體懸浮性為最佳,以球型氧化鋅在細胞毒性測試及抗菌實驗,Hs68細胞能忍受球形氧化鋅微粒之最高及大腸桿菌最小抑菌濃度分別為1.6x102 In this study, the zinc oxide products have been synthesized by using a hydrothermal method. Moreover, the size and morphology of as-prepared ZnO particles differed much from each other and were found be strongly dependent on the pH values of solutions, species and concentration of precipitation agent, and agitation. The zinc oxide products having different morphologies ( lower-like, sphere, pencil-like, shrt hexagonal cylinder) were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), photoluminescence (PL) spectroscopy, Brunauer–Emmett–Teller (BET). In addition, the antibacterial activity and cytotoxicity of wurtzite zinc oxide products were investigated. It is found that the spherical ZnO nanoparticles exhibited the lowest cytotoxicity to human foreskin fibroblast (Hs68) when the ZnO concentration was lower than of 1.6x102μg/ml, and the minimum inhibitory concentration (MIC) in antibacterial activity of Escherichia. Coli (E.Coli) was 3.2x102μg/ml using broth dilution method .Adding spherical ZnO nanoparticles in commercial polymer solution to form liquid barrier film, which liquid barrier film dries quickly to form a breathable, transparent coating on the surface of everything in the air. The effect of ZnO nanoparticles in polymer solution were investigated under ultraviolet A (UV-A) irradiation. The results indicated that Hs68 cell growth index with ZnO nanoparticles was 1.4 times than without ZnO as irradiated time for 30min. Finally, we found that the apoptotic pathway in Hs68 cell using apoptotic genes (TP53, Bax, Bcl-2, Caspase-3). The result indicates the ZnO adding in polymer solution can be protect Hs 68 cell and then reduce cell apoptosis under UV-A irradiation
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