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玉檀木超臨界萃取物對人類肺腺癌細胞生長、移行與侵襲之抑制效果 = Inh...

國立高雄大學生命科學系碩士班

玉檀木超臨界萃取物對人類肺腺癌細胞生長、移行與侵襲之抑制效果 = Inhibitory effects of Bulnesia sarmientoi supercritical fluid extract on the proliferation, migration and invasion of human lung adenocarcinoma cells

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Inhibitory effects of Bulnesia sarmientoi supercritical fluid extract on the proliferation, migration and invasion of human lung adenocarcinoma cells
作者:	張榮哲,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	2014[民103]
面頁冊數:	121面圖，表 : 30公分;
標題:	玉檀木
標題:	Bulnesia sarmientoi
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/62099856308695924194
附註:	106年4月25日公開
附註:	參考書目：面95-112
摘要註:	玉檀木是一種成長緩慢的硬木樹種，主要分佈於南美洲，其傳統療效為消風、熱、毒之功用，以及促使皮膚癒合、開胃、利脾、作為大腦興奮劑等，且有研究指出玉檀木具有抗發炎及抗癌等生理活性。本研究目的是利用體外細胞培養模式，研究以超臨界萃取之玉檀木萃取物 (BSE) 對人類肺腺癌細胞A549、H661、H1299 與人類正常胚胎肺纖維細胞 MRC-5的生長抑制作用及誘導細胞壞死之效應。實驗結果顯示，在處理時間為48 hr 之條件下， BSE 對A549、H661和 H1299 細胞之毒殺IC50 值分別為 43.7、44.6 和53.3 μg/ml，且隨劑量增加，其抑制癌細胞生長的作用愈明顯；相對地，BSE 對 MRC-5 正常細胞的毒殺作用則較低，其 IC50 值為 89.7 μg/ml。再者，BSE 處理時間與癌細胞生長的抑制程度呈正相關，顯示其為一長效型抑癌劑。在細胞週期與細胞凋亡分析方面，BSE 會促使肺癌細胞停滯於 G2/M 生長期。進一步利用Annexin-V-FITC/PI 進行細胞凋亡分析，實驗結果顯示BSE 主要誘導細胞走向壞死。經西方墨點法進一步確認，BSE 會向上調節TNF-α、RIP-1與RIP-3 等促程序性壞死蛋白的表現，並向下調節抗程序性壞死蛋白caspase-8 的表現，證實BSE 會誘導肺癌細胞走向程序性細胞壞死的路徑。此外，由細胞遷移、移行與侵襲試驗結果顯示，BSE有抑制 A549、H661 細胞轉移之能力，並由西方墨點法進一步確認BSE 的處理會抑制MMP-2 及MMP-9 促轉移蛋白的表現。綜合本研究的結果，由於BSE對 A549、H661細胞具有顯著的毒殺活性，且能有效抑制肺癌細胞的轉移，因此BSE具有作為抗肺腺癌藥物之潛力。 Bulnesia sarmientoi tree, which mainly distributed in South America, is a slow-growing hardwood species. The traditional use of B. sarmientoi can eliminate wind, heat, and anti-toxic function, as well as to promote skin healing, appetizers, spleen function, and brain doping. Previous studies indicate that B. sarmientoi has anti-inflammatory and anti-cancer activities. The goal of this study is to investigate the anti-proliferation effects of B. sarmientoi supercritical fluid extract (BSE) against A549, H661, H1299 human lung cancer cells and normal embryonic lung fibroblast cells MRC-5 in vitro, and its necroptotic property. The experimental results showed that the IC50 are 43.7, 44.6 and 53.3 μg/ml, repectively, for A549, H661 and H1299 cells under 48 hr treatment of BSE. In comparison, the IC50 is 89.7 μg/ml for MRC-5 normal cells. This implies that BSE has the cytototic specificity on the cancer cells. Additionally, a positive correlation existed between the treatment time and the inhibition of cell growth, indicating that BSE is a long-duration type of tumor suppressor agent. The flow cytometric analysis indicated that BSE could arrest cancer cells in G2/M phase of the cell cycle. Furthermore, the necroptotic effect of BSE on A549 and H661 cells was confirmed by annexin V-FITC/PI double staining. BSE induced lung cancer cells to the necroptosis pathway was further verified by Western blot analysis, since the pro-necroptotic TNF-α, RIP-1 and RIP-3 proteins were down-regulated and the anti- necroptotic caspase-8 was up-regulated. From the assays of wound healing, migration and invasion, BSE was proved to have a significant inhibitory activity on the migration of lung cancer cells. The results of Western blot analysis showed that the BSE treatment down-regulated the expression of MMP-2 and MMP-9. Taken together, since BSE possesses significant cytotoxicity on A549 and H661 cells and inhibitory activity on the metastasis of lung cancer cells, it may serve as a potential target for the treatment of lung cancer.

玉檀木超臨界萃取物對人類肺腺癌細胞生長、移行與侵襲之抑制效果 = Inhibitory effects of Bulnesia sarmientoi supercritical fluid extract on the proliferation, migration and invasion of human lung adenocarcinoma cells
張, 榮哲

玉檀木超臨界萃取物對人類肺腺癌細胞生長、移行與侵襲之抑制效果 = Inhibitory effects of Bulnesia sarmientoi supercritical fluid extract on the proliferation, migration and invasion of human lung adenocarcinoma cells / 張榮哲撰 - [高雄市] : 撰者, 2014[民103]. - 121面 ; 圖，表 ; 30公分.
106年4月25日公開參考書目：面95-112.
玉檀木Bulnesia sarmientoi

玉檀木超臨界萃取物對人類肺腺癌細胞生長、移行與侵襲之抑制效果 = Inhibitory effects of Bulnesia sarmientoi supercritical fluid extract on the proliferation, migration and invasion of human lung adenocarcinoma cells
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