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綠寶麗魚腦細胞株(ARB8-2)神經細胞標誌表現之研究 = Neural...

國立高雄大學生命科學系碩士班

綠寶麗魚腦細胞株(ARB8-2)神經細胞標誌表現之研究 = Neural marker expression of a clonal cell line (ARB8-2) isolated from the brain of cichlid fish (Aequidens rivulatus)

紀錄類型:	書目-語言資料,印刷品 : 單行本
並列題名:	Neural marker expression of a clonal cell line (ARB8-2) isolated from the brain of cichlid fish (Aequidens rivulatus)
作者:	葉上暐,
其他團體作者:	國立高雄大學
出版地:	[高雄市]
出版者:	撰者;
出版年:	2014[民103]
面頁冊數:	105面圖，表 : 30公分;
標題:	ARB8-2
標題:	ARB8-2
電子資源:	http://handle.ncl.edu.tw/11296/ndltd/10245114111652705879
附註:	參考書目：面74-86
附註:	103年12月16日公開
摘要註:	ARB8-2為綠寶麗魚(Aequidens rivulatus)腦組織所分離出的細胞株，為了解此細胞株是否具有神經幹細胞的特性及其分化潛能，本研究使用反轉錄聚合酶連鎖反應(reverse transcription polymerase chain reaction, RT-PCR)、西方點墨法(Western blot)、細胞免疫染色法(immunocytochemistry)、聚合酶連鎖反應(PCR)及即時聚合酶連鎖反應(real-time PCR)等方法鑑定ARB8-2細胞株的分子表現。結果顯示ARB8-2細胞會表現放射狀神經細胞(radial glia)之標記，如nestin及brain lipid binding protein (BLBP)，星狀神經膠細胞(astrocyte)之標記，如glial fibrillary acidic protein (GFAP)、glutamine synthetase (GS)及vimentin，寡突神經膠前驅細胞(oligodendrocyte progenitor cell)之標記A2B5，神經元之標記tyrosine hydroxylase (TH)，上皮細胞之標記，如gap junction connexin 43 (Cx43)及tight junction occludin，轉錄因子NKX 2.3、NKX6.2及GATA 3。此外，ARB8-2細胞也同時表現黑色素細胞(melanocytes)與視網膜上皮細胞之標記，如tyrosinase、tyrosinase-related protein-2 (TRP-2)、microphthalmia-associated 2 transcription factor (MITF)、SLC45A2、keratinocyte-associated transmembrane protein 2 (KCT-2)及retinal pigment epithelium-specific 65 (RPE65)。根據實驗結果，ARB8-2 可能是一種神經幹細胞。 A clonal cell line (ARB8-2) isolated from the brain of cichlid fish (Aequidens rivulatus) was investigated by reverse transcription polymerase chain reaction (RT-PCR),Western blot, immunocytochemistry, PCR and Real-time PCR were utilized to characterize the molecular expression of ARB8-2 cell line. The results showed that the ARB8-2 express radial glia markers such as nestin and brain lipid binding protein (BLBP), astrocyte markers such as glial fibrillary acidic protein (GFAP), glutamine synthetase (GS) and vimentin, oligodendrocyte progenitor cell marker such as A2B5, neuron markers such as tyrosine hydroxylase (TH), epithelial cell markers such as gap junction connexin 43 (Cx43) and tight junction occludin, melanocyte markers such as tyrosinase, tyrosinase-related protein-2 (TRP-2), microphthalmia-associated transcription factor (MITF), SLC45A2 and keratinocyte-associated transmembrane protein 2 (KCT-2) and retinal epithelial marker retinal pigment epithelium-specific 65 (RPE65). However, the ARB8-2 also express homeodomain-containing transcription factors NKX 2.3 and NKX 6.2 and GATA 3. The results indicated that ARB8-2 might be the neural stem cell.

綠寶麗魚腦細胞株(ARB8-2)神經細胞標誌表現之研究 = Neural marker expression of a clonal cell line (ARB8-2) isolated from the brain of cichlid fish (Aequidens rivulatus)
葉, 上暐

綠寶麗魚腦細胞株(ARB8-2)神經細胞標誌表現之研究 = Neural marker expression of a clonal cell line (ARB8-2) isolated from the brain of cichlid fish (Aequidens rivulatus) / 葉上暐撰 - [高雄市] : 撰者, 2014[民103]. - 105面 ; 圖，表 ; 30公分.
參考書目：面74-86103年12月16日公開.
ARB8-2ARB8-2

綠寶麗魚腦細胞株(ARB8-2)神經細胞標誌表現之研究 = Neural marker expression of a clonal cell line (ARB8-2) isolated from the brain of cichlid fish (Aequidens rivulatus)
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330 $a ARB8-2為綠寶麗魚(Aequidens rivulatus)腦組織所分離出的細胞株，為了解此細胞株是否具有神經幹細胞的特性及其分化潛能，本研究使用反轉錄聚合酶連鎖反應(reverse transcription polymerase chain reaction, RT-PCR)、西方點墨法(Western blot)、細胞免疫染色法(immunocytochemistry)、聚合酶連鎖反應(PCR)及即時聚合酶連鎖反應(real-time PCR)等方法鑑定ARB8-2細胞株的分子表現。結果顯示ARB8-2細胞會表現放射狀神經細胞(radial glia)之標記，如nestin及brain lipid binding protein (BLBP)，星狀神經膠細胞(astrocyte)之標記，如glial fibrillary acidic protein (GFAP)、glutamine synthetase (GS)及vimentin，寡突神經膠前驅細胞(oligodendrocyte progenitor cell)之標記A2B5，神經元之標記tyrosine hydroxylase (TH)，上皮細胞之標記，如gap junction connexin 43 (Cx43)及tight junction occludin，轉錄因子NKX 2.3、NKX6.2及GATA 3。此外，ARB8-2細胞也同時表現黑色素細胞(melanocytes)與視網膜上皮細胞之標記，如tyrosinase、tyrosinase-related protein-2 (TRP-2)、microphthalmia-associated 2 transcription factor (MITF)、SLC45A2、keratinocyte-associated transmembrane protein 2 (KCT-2)及retinal pigment epithelium-specific 65 (RPE65)。根據實驗結果，ARB8-2 可能是一種神經幹細胞。 A clonal cell line (ARB8-2) isolated from the brain of cichlid fish (Aequidens rivulatus) was investigated by reverse transcription polymerase chain reaction (RT-PCR),Western blot, immunocytochemistry, PCR and Real-time PCR were utilized to characterize the molecular expression of ARB8-2 cell line. The results showed that the ARB8-2 express radial glia markers such as nestin and brain lipid binding protein (BLBP), astrocyte markers such as glial fibrillary acidic protein (GFAP), glutamine synthetase (GS) and vimentin, oligodendrocyte progenitor cell marker such as A2B5, neuron markers such as tyrosine hydroxylase (TH), epithelial cell markers such as gap junction connexin 43 (Cx43) and tight junction occludin, melanocyte markers such as tyrosinase, tyrosinase-related protein-2 (TRP-2), microphthalmia-associated transcription factor (MITF), SLC45A2 and keratinocyte-associated transmembrane protein 2 (KCT-2) and retinal epithelial marker retinal pigment epithelium-specific 65 (RPE65). However, the ARB8-2 also express homeodomain-containing transcription factors NKX 2.3 and NKX 6.2 and GATA 3. The results indicated that ARB8-2 might be the neural stem cell.
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