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The Role of the period Protein in Dr...

Brandeis University.

The Role of the period Protein in Drosophila Circadian Rhythms: -Exploring the Effects of the Original perS and perL Mutations.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The Role of the period Protein in Drosophila Circadian Rhythms: -Exploring the Effects of the Original perS and perL Mutations.
Author:	Li, Yue.
Description:	170 p.
Notes:	Source: Dissertation Abstracts International, Volume: 75-06(E), Section: B.
Notes:	Adviser: Michael Rosbash.
Contained By:	Dissertation Abstracts International75-06B(E).
Subject:	Biology.
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3611638
ISBN:	9781303725333

The Role of the period Protein in Drosophila Circadian Rhythms: -Exploring the Effects of the Original perS and perL Mutations.
Li, Yue.

The Role of the period Protein in Drosophila Circadian Rhythms: -Exploring the Effects of the Original perS and perL Mutations. - 170 p.

Source: Dissertation Abstracts International, Volume: 75-06(E), Section: B.

Thesis (Ph.D.)--Brandeis University, 2014.

This item is not available from ProQuest Dissertations & Theses.

The period protein (PER) is a well-studied repressor of clock gene transcription in Drosophila. Two of the original three mutants, period-Short (per S) and period-Long (perL ), dramatically alter the rhythms of flies. However, the mechanisms that underlie these missense mutations and how they affect PER function are not well-understood. In this work, we show that the degradation of PERS is significantly faster than PER both in cell culture and in flies. Because PER as well as PERS is stabilized by protein TIMELESS (TIM), this difference only occurs after TIM degradation. The different degradation rates of PER and PERS underlie the different responses to light of wild-type and per S flies. We also found that the arrhythmic phenotype caused by the loss of neuropeptide PDF in pdf-null animals can be rescued by the perS mutant. By studying this phenomenon, a connection between PDF and PER stability via the activation of PKA was discovered. Although the data about the perL mutation presented in this thesis are still descriptive rather than conclusive, it is quite clear that this mutation causes a series of changes in the transcription of clock genes as well as the CLK-PER and CLK-DNA interactions, the modification of CLK and the binding partners of CLK.

ISBN: 9781303725333Subjects--Topical Terms:

188667
Biology.

The Role of the period Protein in Drosophila Circadian Rhythms: -Exploring the Effects of the Original perS and perL Mutations.
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100 1 $a Li, Yue. $3 708633
245 1 4 $a The Role of the period Protein in Drosophila Circadian Rhythms: -Exploring the Effects of the Original perS and perL Mutations.
300 $a 170 p.
500 $a Source: Dissertation Abstracts International, Volume: 75-06(E), Section: B.
500 $a Adviser: Michael Rosbash.
502 $a Thesis (Ph.D.)--Brandeis University, 2014.
506 $a This item is not available from ProQuest Dissertations & Theses.
506 $a This item must not be sold to any third party vendors.
520 $a The period protein (PER) is a well-studied repressor of clock gene transcription in Drosophila. Two of the original three mutants, period-Short (per S) and period-Long (perL ), dramatically alter the rhythms of flies. However, the mechanisms that underlie these missense mutations and how they affect PER function are not well-understood. In this work, we show that the degradation of PERS is significantly faster than PER both in cell culture and in flies. Because PER as well as PERS is stabilized by protein TIMELESS (TIM), this difference only occurs after TIM degradation. The different degradation rates of PER and PERS underlie the different responses to light of wild-type and per S flies. We also found that the arrhythmic phenotype caused by the loss of neuropeptide PDF in pdf-null animals can be rescued by the perS mutant. By studying this phenomenon, a connection between PDF and PER stability via the activation of PKA was discovered. Although the data about the perL mutation presented in this thesis are still descriptive rather than conclusive, it is quite clear that this mutation causes a series of changes in the transcription of clock genes as well as the CLK-PER and CLK-DNA interactions, the modification of CLK and the binding partners of CLK.
590 $a School code: 0021.
650 4 $a Biology. $3 188667
650 4 $a Neurosciences. $3 211508
650 4 $a Molecular biology. $3 188737
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710 2 $a Brandeis University. $b Molecular and Cell Biology. $3 708634
773 0 $t Dissertation Abstracts International $g 75-06B(E).
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856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3611638