| 摘要註: |
藉由人體尿液中的黃體素、雌二醇及睪固酮之生理濃度評估人體之性腺疾病及更年期後引發的高血壓、心血管疾病、糖尿病、肥胖等相關疾病。在本實驗中,以氧化還原聚合方式合成導電性高分子材料(聚苯胺間-胺基苯磺酸),再結合分子拓印技術進行黃體素、雌二醇及睪固酮分子拓印於自組裝聚苯胺間-胺基苯磺酸薄膜,並且將此薄膜製備成感測電極。從實驗結果得知黃體素、睪固酮及雌二醇分子拓印聚苯胺間-胺基苯磺酸薄膜之拓印濃度分別為10及1 ug/ml,並摻雜間-胺基苯磺酸莫爾比為66.67 及50.00 mole%時有較良好的拓印效率。將此拓印參數作為電極感測元件,黃體素、雌二醇及睪固酮線性濃度範圍分別為3.2-318.0、3.7-367.1、3.5-346.7 pM,最低偵測極限分別為2.1、0.9、0.7 pM,也將此感測元件進行干擾測試,其結果證明此分子拓印薄膜具有相當的專一選擇性。針對於尿液檢體中的目標荷爾蒙進行電化學量測,另外將此尿液檢體經由醫院醫檢部以冷光化學免疫分析法進行目標荷爾蒙濃度之檢測,將其濃度作為基準與電化學量測結果比較,其準確率分別為87.2± 7.5、84.5± 3.6及85.9± 4.3 %。 The abnormal physiological levels of progesterone, 17b-estradiol, testosterone may correlate with human gonadal disorders and hypertension, cardiovascular disease, diabetes, obesity after menopause. Non-invasive detection is rapid and convenient method to monitor physiological concentration of hormones. In this work, three target molecules (progesterone, 17b-estradiol, testosterone) can be imprinted into poly (aniline-co-metanilic acid) via redox polymerization self-assemble on the working electrode of electrochemical sensing chips, then used as the tool for optimizing imprinting parameters. The highest imprinting effectivenesses were found when imprinting concentrations of the target molecules were 10, 1, 10 ug/ml by doping 66.67, 50 and 50 mole% of metanilic acid, respectively. The linear detection ranges of imprinted targets exhibit in 3.2-318.0, 3.7-367.1 and 3.5-346.7 pM, with detection limit were 2.1, 0.9, 0.7 pM. In the electrochemical urinalysis, several real samples were measured and compared with the commercial instrument ARCHITECT ci 8200 system. The accuracies using the molecularly imprinted polymers sensing system for progesterone, 17b-estradiol and testosterone achieved 87.2± 7.5 %、84.5± 3.6 %及85.9± 4.3 %, respectively. |