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Biotechnologies for plant mutation b...

Jankowicz-Cieslak, Joanna.

Biotechnologies for plant mutation breedingprotocols /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Biotechnologies for plant mutation breedingedited by Joanna Jankowicz-Cieslak ... [et al.].
Reminder of title:	protocols /
other author:	Jankowicz-Cieslak, Joanna.
Published:	Cham :Springer International Publishing :2017.
Description:	xx, 340 p. :ill. (some col.), digital ;24 cm.
Contained By:	Springer eBooks
Subject:	Plant mutation breeding.
Online resource:	http://dx.doi.org/10.1007/978-3-319-45021-6
ISBN:	9783319450216$q(electronic bk.)

Biotechnologies for plant mutation breedingprotocols /
Biotechnologies for plant mutation breedingprotocols /[electronic resource] :edited by Joanna Jankowicz-Cieslak ... [et al.]. - Cham :Springer International Publishing :2017. - xx, 340 p. :ill. (some col.), digital ;24 cm.

Mutagenesis for Crop Breeding and Functional Genomics -- Chemical and Physical Mutagenesis in Jatropha curcas -- Chemical Mutagenesis and Chimera Dissolution in Vegetatively Propagated Banana -- Mutation Induction Using Gamma Irradiation and Embryogenic Cell Suspensions in Plantain (Musa spp.) -- Optimization of Somatic Embryogenesis in Cassava -- Creation of a TILLING Population in Barley after Chemical Mutagenesis with Sodium Azide and MNU -- Site-Directed Mutagenesis in Barley by Expression of TALE Nuclease in Embryogenic Pollen -- Doubled Haploidy as a Tool for Chimera Dissolution of TALEN-Induced Mutations in Barley -- Field Evaluation of Mutagenized Rice Material -- Root Phenotyping Pipeline for Cereal Plants -- Breeding New Aromatic Rice with High Iron using Gamma Radiation and Hybridization -- Utilizing NIRS for Qualitative and Non-Destructive Identification of Seed Mutants in Large Populations -- Protocols for Proteome Analyses of Jatropha curcas -- Low-Cost Methods for DNA Extraction and Quantification -- A Protocol for Benchtop Extraction of Single-Strand-Specific Nucleases for Mutation Discovery -- A Protocol for Validation of Doubled Haploid Plants by Enzymatic Mismatch Cleavage -- Bioinformatics-Based Assessment of the Relevance of Candidate Genes for Mutation Discovery -- Mutation Detection by Analysis of DNA Heteroduplexes in TILLING Populations of Diploid Species -- Determining Mutation Density using Restriction Enzyme Sequence Comparative Analysis (RESCAN) -- Next-Generation Sequencing for Targeted Discovery of Rare Mutations in Rice.

Open access.

This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.

ISBN: 9783319450216$q(electronic bk.)

Standard No.: 10.1007/978-3-319-45021-6doiSubjects--Topical Terms:

771817
Plant mutation breeding.

LC Class. No.: SB123

Dewey Class. No.: 631.52

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245 0 0 $a Biotechnologies for plant mutation breeding $h [electronic resource] : $b protocols / $c edited by Joanna Jankowicz-Cieslak ... [et al.].
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300 $a xx, 340 p. : $b ill. (some col.), digital ; $c 24 cm.
505 0 $a Mutagenesis for Crop Breeding and Functional Genomics -- Chemical and Physical Mutagenesis in Jatropha curcas -- Chemical Mutagenesis and Chimera Dissolution in Vegetatively Propagated Banana -- Mutation Induction Using Gamma Irradiation and Embryogenic Cell Suspensions in Plantain (Musa spp.) -- Optimization of Somatic Embryogenesis in Cassava -- Creation of a TILLING Population in Barley after Chemical Mutagenesis with Sodium Azide and MNU -- Site-Directed Mutagenesis in Barley by Expression of TALE Nuclease in Embryogenic Pollen -- Doubled Haploidy as a Tool for Chimera Dissolution of TALEN-Induced Mutations in Barley -- Field Evaluation of Mutagenized Rice Material -- Root Phenotyping Pipeline for Cereal Plants -- Breeding New Aromatic Rice with High Iron using Gamma Radiation and Hybridization -- Utilizing NIRS for Qualitative and Non-Destructive Identification of Seed Mutants in Large Populations -- Protocols for Proteome Analyses of Jatropha curcas -- Low-Cost Methods for DNA Extraction and Quantification -- A Protocol for Benchtop Extraction of Single-Strand-Specific Nucleases for Mutation Discovery -- A Protocol for Validation of Doubled Haploid Plants by Enzymatic Mismatch Cleavage -- Bioinformatics-Based Assessment of the Relevance of Candidate Genes for Mutation Discovery -- Mutation Detection by Analysis of DNA Heteroduplexes in TILLING Populations of Diploid Species -- Determining Mutation Density using Restriction Enzyme Sequence Comparative Analysis (RESCAN) -- Next-Generation Sequencing for Targeted Discovery of Rare Mutations in Rice.
506 $a Open access.
520 $a This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.
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650 0 $a Plant biotechnology. $3 188724
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650 2 4 $a Plant Breeding/Biotechnology. $3 275234
650 2 4 $a Agriculture. $3 274257
650 2 4 $a Genetic Engineering. $3 189027
650 2 4 $a Plant Genetics & Genomics. $3 274273
700 1 $a Jankowicz-Cieslak, Joanna. $3 771816
710 2 $a SpringerLink (Online service) $3 273601
773 0 $t Springer eBooks
856 4 0 $u http://dx.doi.org/10.1007/978-3-319-45021-6
950 $a Biomedical and Life Sciences (Springer-11642)