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Rapid cell magnetisation using catio...

Carreira, Sara Correia.

Rapid cell magnetisation using cationised magnetoferritin

Record Type:	Electronic resources : Monograph/item
Title/Author:	Rapid cell magnetisation using cationised magnetoferritinby Sara Correia Carreira.
Author:	Carreira, Sara Correia.
Published:	Cham :Springer International Publishing :2017.
Description:	xvii, 166 p. :ill., digital ;24 cm.
Contained By:	Springer eBooks
Subject:	Biophysics.
Online resource:	http://dx.doi.org/10.1007/978-3-319-60333-9
ISBN:	9783319603339$q(electronic bk.)

Rapid cell magnetisation using cationised magnetoferritin
Carreira, Sara Correia.

Rapid cell magnetisation using cationised magnetoferritin[electronic resource] /by Sara Correia Carreira. - Cham :Springer International Publishing :2017. - xvii, 166 p. :ill., digital ;24 cm. - Springer theses,2190-5053. - Springer theses..

Introduction -- Materials and Methods -- Characterising Magnetoferritin and Cationised Magnetoferritin -- Stem Cell Labelling with Cationised Magnetoferritin -- Toxicological Profiling of Cationised Magnetoferritin -- Escherichia coli labelling with Cationised Magnetoferritin -- Overall Conclusions and Future Opportunities.

Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.

ISBN: 9783319603339$q(electronic bk.)

Standard No.: 10.1007/978-3-319-60333-9doiSubjects--Topical Terms:

188608
Biophysics.

LC Class. No.: QH505

Dewey Class. No.: 571.47

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505 0 $a Introduction -- Materials and Methods -- Characterising Magnetoferritin and Cationised Magnetoferritin -- Stem Cell Labelling with Cationised Magnetoferritin -- Toxicological Profiling of Cationised Magnetoferritin -- Escherichia coli labelling with Cationised Magnetoferritin -- Overall Conclusions and Future Opportunities.
520 $a Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.
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650 1 4 $a Physics. $3 179414
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650 2 4 $a Stem Cells. $3 203924
650 2 4 $a Magnetism, Magnetic Materials. $3 274439
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