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Advancing development of synthetic g...

Chandran, Anandhakumar.

Advancing development of synthetic gene regulatorswith the power of high-throughput sequencing in chemical biology /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Advancing development of synthetic gene regulatorsby Anandhakumar Chandran.
Reminder of title:	with the power of high-throughput sequencing in chemical biology /
Author:	Chandran, Anandhakumar.
Published:	Singapore :Springer Singapore :2018.
Description:	xv, 114 p. :ill. (some col.), digital ;24 cm.
Contained By:	Springer eBooks
Subject:	Genetic regulation.
Online resource:	http://dx.doi.org/10.1007/978-981-10-6547-7
ISBN:	9789811065477$q(electronic bk.)

Advancing development of synthetic gene regulatorswith the power of high-throughput sequencing in chemical biology /
Chandran, Anandhakumar.

Advancing development of synthetic gene regulatorswith the power of high-throughput sequencing in chemical biology /[electronic resource] :by Anandhakumar Chandran. - Singapore :Springer Singapore :2018. - xv, 114 p. :ill. (some col.), digital ;24 cm. - Springer theses,2190-5053. - Springer theses..

Overview of Next-Generation Sequencing Technologies and its application in Chemical Biology -- Next Generation Sequencing Studies Guide the Design of Pyrrole-Imidazole Polyamides with Improved Binding Specificity by the Addition of β-alanine -- Genome-Wide Assessment of the Binding Effects of Artificial Transcriptional Activators by Utilizing the Power of High-Throughput Sequencing -- Deciphering the genomic targets of alkylating polyamide conjugates using high-throughput sequencing.

This book focuses on an "outside the box" notion by utilizing the powerful applications of next-generation sequencing (NGS) technologies in the interface of chemistry and biology. In personalized medicine, developing small molecules targeting a specific genomic sequence is an attractive goal. N-methylpyrrole (P)-N-methylimidazole (I) polyamides (PIPs) are a class of small molecule that can bind to the DNA minor groove. First, a cost-effective NGS (ion torrent platform)-based Bind-n-Seq was developed to identify the binding specificity of PIP conjugates in a randomized DNA library. Their biological influences rely primarily on selective DNA binding affinity, so it is important to analyze their genome-wide binding preferences. However, it is demanding to enrich specifically the small-molecule-bound DNA without chemical cross-linking or covalent binding in chromatinized genomes. Herein is described a method that was developed using high-throughput sequencing to map the differential binding sites and relative enriched regions of non-cross-linked SAHA-PIPs throughout the complex human genome. SAHA-PIPs binding motifs were identified and the genome-level mapping of SAHA-PIPs-enriched regions provided evidence for the differential activation of the gene network. A method using high-throughput sequencing to map the binding sites and relative enriched regions of alkylating PIP throughout the human genome was also developed. The genome-level mapping of alkylating the PIP-enriched region and the binding sites on the human genome identifies significant genomic targets of breast cancer. It is anticipated that this pioneering low-cost, high through-put investigation at the sequence-specific level will be helpful in understanding the binding specificity of various DNA-binding small molecules, which in turn will be beneficial for the development of small-molecule-based drugs targeting a genome-level sequence.

ISBN: 9789811065477$q(electronic bk.)

Standard No.: 10.1007/978-981-10-6547-7doiSubjects--Topical Terms:

192968
Genetic regulation.

LC Class. No.: QH450

Dewey Class. No.: 572.865

Advancing development of synthetic gene regulatorswith the power of high-throughput sequencing in chemical biology /
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